Saratov JOURNAL of Medical and Scientific Research

Problems and ways to improve the laboratory diagnosis of chlamydial infection


The review highlights the historical aspects and modern concepts of laboratory diagnosis of chlamydial infection. The studies on investigation of different strains of Chlamydia trachomatis performed in Russian Federation and abroad are summarized. The results of research of domestic and foreign scientists aimed at finding the perfect laboratory test for identification of C. trachomatis on the basis of molecular biological methods are analyzed and presented.

1. Halberstaedter L, von Prowazek S. Zur Atiologie des Tra-choms (in German). Deutsch Med Wochenschr 1907; 33: 1285-1287
2. Lindner K. Zur aetiologie der gonokokkenfrien urethritis. Wiener klinische Wochenschrift 1910; 23: 283-284
3. Jones H, Rake G, and Steams B. Studies on lymphogran-uloma venerum. III. The action of the sulfonamides on the agent of lymphogranuloma venerum. Journal of Infectious Diseases 1945;76:55-69
4. Page LA. Revision of the family Chlamydiaceae Rake (Rickettsiales): unification of the psittacosis-lymphogranuloma venerum-trachoma group of organisms in the genus Chlamydia Jones, Rake, and Steams, 1945. Int J Syst Bacteriol 1966; 16: 223-252
5. Page LA. Proposal for the recognition of two species in the genus Chlamydia Jones, Rake, and Steams, 1945. Int J Syst Bacteriol 1968; 18:51-66
6. Storz J, Page LA. Taxonomy of the chlamydiae: reasons for classifying organisms of the genus Chlamydia, family Chlamydiaceae, in a separate order, Chlamydiales. Int J Syst Bacteriol 1971;21:332-334
7. Tang FF, Chang HL, Hiang YT, et al. Studies on the etiology of trachoma with special reference to isolation of the virus in chick embryo. Chin Med J 1957; 75: 429-447
8. Collier LH, Sowa J. Isolation of trachoma virus in embryo-nated eggs. Lancet 1958; 1: 993-996
9. Collier LH, Duke-Elder S, Jones BR. Experimental trachoma produced by cultured virus. British Journal of Ophthalmology 1958;42:705-720
10. Jones BR, Collier LH and Smith CH. Isolation of virus from inclusion blennorrhea. Lancet 1959; 1: 902-905
11. Jones BR.AI-Hussaini MK, Dunlop EMC. Genital infection in association with TRIC virusinfection of the eye. I. Isolation of virus from urethra, cervix, andeye: preliminary report. Br J Ven-erDis1964;40: 19-24
12. Dunlop EMC, Harper IA, Al-Hussaini MK, et al. Relation to TRIC agent to "non specific" genital infection. Br J Vener Dis 1966; 42: 77-87
13. Dunlop EMC, Freedman A, Garland JA, et al. Infection by Bedsoniae and the possibility of spurious isolation: 2. Genital infection, disease of the eye, Reiter's disease. Amer. J Ophthal-mol 1967; 63: 1073-81
14. Thygeson P, Hanna L, Dawson C, et al. Inoculation of human volunteer with egg-grown inclusion conjunctivitis virus. Am J Ophtalmol 1962 May; 53: 786-795
15. Gordon FB, Quan AL. Isolation of the trachoma agent in cell culture. Proc Soc Exp Biol Med 1965 Feb; 118: 354-359
16. Dunlop EM, Hare MJ, Darougar S, et al. Detection of Chlamydia (Bedsonia) in certain infections of man. II. Clinical study of genital tract, eye, rectum, and other sites of recovery of Chlamydia. J Infect Dis 1969 Oct; 120 (4): 463-470
17. Shatkin AA, Mavrov II. Urogenital chlamydio-sis. Kiev: Health, 1983; 14 p.
18. Ripa КТ, Mardh PA. Cultivation of СТ in cyclohexim-ide-treated mccoy cells. J Clin Microbiol 1977 Oct; 6 (4): 328-331
19. Dmitriev G.A. Urogenital Chlamydial infection: approaches to diagnosis and therapy. Clin Dermatol and Vene-rol 2003; 2: 24-28
20. Popovich GG. The study of antigens obtained by ultrasonic disintegration of Chlamydia in the RSK cross. J Microbiol 1978; 40 (4): 477-479
21. Wang S-P. A micro-immunofluorescence method. А study of antibody response to TRIC organisms in mice. In: Trachoma and Related Disorders caused by Chlamydial Agents. Nichols R L, ed. Amsterdam: Excerpta Medica, 1971; p. 273-288
22. Dwyer RStC, Treharne JD, Jones BR, et al. Chlamydial infection. Results of microimmunofluorescence tests for the detection of type-specific antibody in certain chlamydial infections. Brit J vener Dis 1972; 48: 452
23. Wang S-P, Grayston JT Human serology in CT infections with micro-immunofluorescence. J Infect Dis 1974; 130: 388-379
24. Stephens RS, Tarn MR, Kuo CC, et al. Monoclonal antibodies to CT: antibody specificities and antigen characterization. J Immunol 1982 Mar; 128 (3): 1083-1089
25. Kutlin AV, Drobyshevskaia El, Shatkin AA. The immu-nochemical and biological properties of monoclonal antibodies to Chlamydia trachomatis. Zh Microbiol Epidemiol Immunobiol, 1996 Jan-Feb; (1):3-6
26. Gomberg MA, Mashkilleyson AL, Delektorkaya VV. Use of antichlamydia antibodies for the diagnosis of urogenital chlamydiosis. Journal of Dermatology 1986; (8): 57-61
27. Shatkin AA, Beskin RS, Pankratov V, et al. Display galprovy antigen (Chlamydia) direct immunoperoxidase method. J Mycrobiol epidemiol immunol 1976; (9): 74-78
28. Duggan MA, Pomnoni С, Kay D, et al. Infantile chlamydial conjunctivitis: A comparison of Papanicolaou, Giems and immunoperoxidase stainin methods. Acta cytol 1986; 30 (4): p. 341-346
29. Lewis VJ, Thacker WL, and Mitchell SH. Enzyme-linked immunosorbent assay for chlamydial antibodies. J Clin Microbiol 1977 Nov; 6 (5): 507-510
30. Skaug K, Vik ISS, Qvigstad E, et al. Chlamydial serum IgG antibodies in patients with acute salpingitis measured by an enzyme-linked immunosorbent assay. Acta Phathol Microbiol Immunol Scand С 1982 Nov; 90 (6): 67-71
31. Evans RT, Taylor-Robinson D. Development and evaluation of an enzyme-linked immunosorbent assay (ELISA), using chlamydial group antigen, to detect antibodies to Chlamydial trachomatis. J Clin Pathol 1982; 35: 1122-1128
32. Cevenini R, Sarov I, Rumpianesi F, et al. Serum specific IgA antibody to CT in patient with chlamydial infections detected by ELISA and an immunofluorescence test. J Clin Pathol 1984 Jun; 37 (6): 686-691
33. Miettinen A, Heinonen PK, Teisala K, et al. Antigen specific serum antibody response to CT in patients with acute pelvic inflammatory disease. J Clin Pathol 1990 Sep; 43 (9): 758-761
34. Samra Z, Softer Y. IgA antichlamydia antibodies as a diagnostic tool for monitoring of active chlamydial infection. Eur J Epidemiol 1992 Nov;8 (6):882-884
35. Kumamoto Y, Sato T, Hiroi M, et al. Assessment of CT-specific IgA and IgG serum antibodies in genitourinary CT infection — comparative study between HITAZYME and IPAzyme. Kansenshogaku Zasshi 1993 Apr; 67 (4): 315-30
36. Maruta N. Study of CT in chronic prostatitis. Hinyokika Kiyo 1992 Mar; 38 (3): 297-304. Japanese
37. Piura B, Sarov B, and Sarov I. Persistence of antichla-mydial antibodies after treatment of acute salpingitis with doxycy-cline. Eur J Obs Gyn Reprod Biol 1993; 48: 117-121
38. Workowski KA, Lampe MF, Wong KG, et al. Long-term eradication of CT genital infection after antimicrobial therapy: Evidence against persistent infection. JAMA 1993 Nov 3; 270 (17): 2071-5
39. Henry-Suchet J, Askienazy-Elbhar M, Thibon M, et al. Post-therapeutic evolution of serum chlamydial antibody titers in women with acute salpingitis and tubal infertility. Fertil Steril 1994 Aug; 62 (2): 296-304
40. Bjercke S, and Purvis K. Chlamydia serology in the investigation of infertility. Hum Reprod 1992; 7: 621-4
41. Wolff H, Neubert U, Volkenandt M, et al. Detection of CT in semen by antibody-enzyme immunoassay compared with polymerase chain reaction, antigen-enzyme immunoassay, and urethral cell culture. Fertil Steril 1994; 62: 1250-1254
42. Dieterle S, Mahony JB, Luinstra KE, et al. Chlamydial immunoglobulin IgG and IgA antibodies in serum and semen are not associated with the presence of CT DNA or rRNA in semen from male partners of infertile couples. Hum Reprod 1995; 10: 315-19
43. Corradi G, Konkoly Thege M, Panovics J, et al. Is semen fluid a suitable specimen for detecting chlamydial infection in men?Acta Microbiol Immunol Hung 1995; 42 (4): 389-94
44. Ludwig M, Hausmann G, Hausmann W, et al. CT antibodies in serum and ejaculate of male patients without acute urethritis. Ann Urol (Paris) 1996; 30 (3): 139-46
45. Bannikova VA. Development of ELISA for the laboratory diagnosis of Chlamydia: PHD diss. Saratov, 2004; 174 p.
46. Fedorova VA, Bannikova VA, Alikberov Sh, et al. Comparative efficacy of detection of the causative agent of urogenital chlamydia immunofluorescence methods, PCR and Dot-im-munoassay. Clinical Laboratory Services 2007; (7): 30-35
47. Fedorova VA, Alikberov Sh, EliseevYY, etal.Acompar-ative study of the effectiveness of methods serodiagnostics uro-genital chlamydiosis. Clinical Laboratory Services 2010; (4): 45-49
48. Selkov SA, Kostyuchek DF, Richuk SV, et al. Seasonal fluctuations in the incidence of chlamydia, mycoplas-mosis and ureaplasmosis. In: Ecology and Development of North-West Russia: Abstracts of scientific reports of the 7th International conference. St. Petersburg, 2002; p. 83-84
49. Kuhtinova NV, Krotov SA, Krotov VA. Recurrent and chronic forms hlamidofileza in children: current standards of diagnosis, clinical features, family rehabilitation scheme: Manual for physicians. Koltsovo, 2004; 150 с. 50. Cohenm CR, Sinei SS, Bukusi EA, et al. Human leukocyte antigen class IIDQ alleles associated with CT tubal infertility. Obstet and Gynec 2000; 95 (1): 72-77
51. Chernesky M, Luinstra K, Sellors J, et al. Can serology diagnose upper genital tract CT infections? Studies on women with pelvic pain, with or without chlamydial plasmid DNA in endo-metrial biopsy tissue. Sex Transm Dis 1998; 25: 14-9
52. Bakulev AL. Syndrome of endogenous intoxication in Reiter's disease and optimization of pharmacotherapy: DSc abstract. Saratov, 2003; 35 p.
53. Palva A, Jousimies-Somer Н, Saikku Р, et al. Detection of CTby nucleic acid sandwich hybridization. FEMS Microbiology Letters 1984; 23(1): 83-89
54. Hyypia T, Jalava A, Larsen SH, et al. Detection of CT in clinical specimens by nucleic acid spot hybridization. J Gen Microbiol 1985 Apr; 131 (4): 975-978
55. Tuokko H, Ruuska P, Hyypia T. Comparison of nucleic acid hybridization with enzyme immunoassay and isolation for detection of CT. Eur J Clin Microbiol Infect Dis 1989 Apr; 8 (4): 320-1
56. Krotov SA, Krotov VA, Yuryev SY Chlamydia: epidemiology, pathogen characteristics, methods of laboratory diagnostics, treatment of genital Chlamydia: Abstract messages. Koltsovo, 1997; 63 p.
57. Shalepo KV, Shipitsyna EV, Savicheva, AM et al. Comparison of methods for the laboratory diagnosis of urogen-ital infections caused by CT. Journal of Obstetrics and gynecological diseases 2001; 50 (4): 77-82
58. Churakov АА, Kulichenko AN, Kazakov ES. On the issue of laboratory diagnosis of urogenital chlamydiosis. Clinical and laboratory diagnosis 2005; (2): 43-47
59. Shipitsyna EV, Vorobyova NE, Savicheva AM, et al. Application of NASBA (Nucleic Acid Sequence-Based Amplification) in real time to diagnose the urogenital chlamydial infection. Journal of Obstetrics and gynecological diseases 2005; 54 (4)
60. Tikhomirov AL, Sarsania SI. Modern approaches to the treatment of pelvic inflammatory disease: Guidelines, M., 2005; 32 p.
61. Trum JW, Pannekoek Y, Spanjaard L, et al. Accurate detection of male subclinical genital tract infection via cervical culture and DNA hybridization assay of the female partner. Int J Andr 2000; 23(1): 43-45
62. Lovett M, Kuo K-K, Holmes K, et al. (1980). Plasmids of the genus Chlamydia. In: Current chemotherapy and infectious diseases, vol. 2, pp. 1250-1252 (Eds Nelson, J & Grassi, C) published by American Society of Microbiology, Washington DC
63. Comanducci M, Ricci S, Cevenini R, et al. Diversity of the CT common plasmid in biovars with different pathogenicity Plasmid 1990; 23: 149-154
64. Thomas NS, Lusher M, Storey CC, et al. Plasmid diversity in Chlamydia. Microbiology 1996 Jun; 143 (Pt6), 1847-1854
65. Comanducci M, Cevenini R, Moroni A, et al. Expression of a plasmid gene of CT encoding a novel 28 kDa antigen. J Gen Microbiol 1993; 139: 1083-1092
66. O'Connell CM, IngallsRR, Andrews CWJr, etal. Plas-mid-deficient Chlamydia muridarum fail to induce immune pathology and protect against oviduct disease. J Immunol 2007; 179: 4027-40348
67. Carlson JH, Whitmore WM, Crane DD, et al. The CT plasmid is a transcriptional regulator of chromosomal genes and a virulence factor. Infect Immun 2008; 76: 2273-22838 68. Peterson EM, Markoff BA, Schachter J, et al. The 7,5 kb plasmid present in CT is not essential for the growth of this microorganism. Plasmid 1990; 23: 144-148
69. Stothard DR, Williams JA, Van Der Pol B, et al. Identification of a CT serovar E urogenital isolate witch lacks the cryptic plasmid. Infect Immun 1998; 66: 6010-6013
70. RipaT, Nilsson P. A variant О trachomatis with deletion in cryptic plasmid: implications for use of PCR diagnostic tests. Euro Surveill 2006; (11): 9-11
71. Ripa T, Nilsson P. A С trachomatis strain with a 377-bp deletion in the cryptic plasmid causing false-negative nucleic acid amplification tests. Sex Transm Dis 2007; 34 (5): 255-6
72. Pedersen LN, Hermann B, Moller JK. Typing С tra-chomatis: from egg yolk to nanotechnology. FEMS Immunol Med Microbiol 2009 Mar; 55 (2): 120-30
73. Marions L, Rotzen-Ostlund M, Grillner L, et al. High occurrence of a new variant of CT escaping diagnostic tests among STI clinic patients in Stockholm, Sweden. Sex Transm Dis 2008 Jan; 35(1): 61-4
74. Shipitsyna E, Hadad R, Ryzhkova O, et al. First reported case of the Swedish new variant of Chlamydia trachomatis (nvCT) in Eastern Europe (Russia), and evaluation of Russian nucleic acid amplification tests regarding their ability to detect nvCT. Acta Derm Venereol 2012 May; 92 (3): 330-1
75. Feodorova VA, Sultanakhmedov ES, Saltykov YuV, et al. The first case of the Swedish new variant Chlamydia trachomatis in the Southeastern European region Russia. In: Proceeding Book, IUSTI Europe Scientific Meeting in St Julian, Malta, September, 18-20. 2014; p. 1068 76. Pashko YP, Zigangirova NA, Kapotina LN, et al. Features of distribution in the organism Chlamydia trachomatis in the chronic course of urogenital chlamydia and pathogen detection in blood serum. Fundamental Research 2010; 7: 50-57
77. Feodorova VA, Sultanakhmedov ES, Saltykov YV, et al. Testing the blood of patients with urogenital chlamydia infection increases the efficiency of laboratory diagnosis of Chlamydia trachomatis. Molecular diagnosis 2014; 1: 172-173
78. Feodorova VA, Konnova SS, Polyanina Tl. Using a variety of targets to identify strains of Chlamydia trachomatis. Proceedings of the Saratov University 2011; 11 (2): 73-76
79. Kubanova АА, ed. Dermatovenerology: Clinical Guidelines. Moscow: ZAO FID Delovoi express, 2010; 413-425.

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