Saratov JOURNAL of Medical and Scientific Research

laboratory diagnostics

Problems and ways to improve the laboratory diagnosis of chlamydial infection


The review highlights the historical aspects and modern concepts of laboratory diagnosis of chlamydial infection. The studies on investigation of different strains of Chlamydia trachomatis performed in Russian Federation and abroad are summarized. The results of research of domestic and foreign scientists aimed at finding the perfect laboratory test for identification of C. trachomatis on the basis of molecular biological methods are analyzed and presented.

2015_03-01_414-421.pdf427.05 KB

Detection of Chlamydia trachomatis in blood samples as a diagnostic method for complicated and persistent forms of urogenital chlamydia infections


Goal: the study of the effectiveness of the method for laboratory diagnostics of urogenital chlamydial infection in patients with chronic form of the disease. Material and methods. The presence of DNAof C. trachomatis was detected by PCR in either genital or extragenital (blood) sites in eighth patients (four men and four women). Results. It is established that in biological material taken from extragenital (blood) sites, C. trachomatis was detected in all patients examined (in 100% of cases), while in clinical samples obtained from genital sites, in seven patients only (87.5%). Conclusion. We found that specific chlamydial DNAcan be detected in extragenital (blood) site, despite the negative reaction in the clinical material from the genital tract of patients with genital chlamydial infection.

2015_03-01_381-385.pdf321.91 KB

First cases of West Nile fever in Saratov region

Year: 2013, volume 9 Issue: №3 Pages: 495-501
Heading: Infectious Diseases Article type: Original article
Authors: Krasovskaya T.Yu., Naydenova E.V., Mironova N.I., Talaeva E.A., Kuklev V.E., Scherbakova S.A., Kutyrev V.V.
Organization: Engels Hospital № 2, Saratov Scientific Research Institute «Microbe»

The aimofthe article is to provide assessment of the role of West Nile virus in the infectious pathology on the territory of the Saratov region. Material and methods. Clinical samples (blood, serum, cerebrospinal fluid, urine) taken from 27 patients with symptoms resembling West Nile fever, receiving treatment in infectious departments of hospitals in Saratov and the Saratov region were analyzed. Results. In 2012 forthe first time in the Saratov region 11 cases ofWest Nile fever were detected. Conclusion. Analysis of the current epidemiological situation on West Nile fever demonstrates the necessity of strengthening of prophylactic and counter-epidemic measures to prevent cases of this disease in the region.

2013-03_495-501.pdf289.78 KB