Saratov JOURNAL of Medical and Scientific Research

Thermovision registration of defecation and urination in the evaluation of emotional state of experimental biological objects in the open arenas of behavioral tests


Purpose: to provide a method for detection of boluses defecation and urination spots in the study of the behavior of experimental biological objects in open arenas ethological tests with thermal registration. Material and methods. For identifying and quantifying defecation and urination biological objects using the compact thermal imaging camera FLIR SC 660 (FLIR Systems) with a wide-angle lens. Object of study is the laboratory rat. Morphometric parameters determined using image analysis software. Results. When used as a video recording device thermal imaging camera to get an objective, quantitative data on the level of defecation and urination in the study of behavior and emotional state of the experimental evaluation of warm-blooded biological objects in open arenas ethological tests. Recorded boluses defecation and urination spots in the study of the emotional component of the behavior of laboratory rats in the test apparatus «Open field», «Elevated plus maze», «Radial 8-Arm Maze» and «T-maze». Conclusion. Thermal video registration allows visualizing products vegetative urinary and fecal excretion in the dynamics of experimental studies in the evaluation of the psycho-emotional state of biological objects in various open arenas behavioral tests, carried out their detailed morphometric analysis and identify indicators of spatial distribution.

1. BureshYa, BureshovaO, Kh'yuston DP. Metodikiiosnovnye eksperimenty po izucheniyu mozga i povedeniya. Moscow: Vys-shaya shkola, 1991; 399 s.
2. Sudakov KV, Umryukhin РЕ. Sistemnye osnovy emot-sional'nogo stressa. Moscow: GEOTAR-Media, 2010; 112 s.
3. Rukovodstvo po provedeniyu doklinicheskikh issledo-vaniy lekarstvennykh sredstv. Chast' pervaya. Pod red. Mironova AN. Moscow: Grif i K, 2012; 944 s.
4. Karkishchenko NN, Uyba VV, Karkishchenko VN, Shustov EB. Ocherki sportivnoy farmakologii. Tom 1: Vektory ekstrapoly-atsii. Moscow: Aysing, 2013; 288 s.
5. Vosstanovitel'naya meditsina. Pod red. Khadartseva AA, Gontareva SN, Kryukovoy SV. Tula: Izd-vo TulGU 2010; T. II; 262 s. 6. Ring EF, Ammer К. Infrared thermal imaging in medicine. Physiol Meas 2012; 33 (3): 33-46 7. Bilodeau GA, Torabi A, Levesque M, et al. Body temperature estimation of a moving subject from thermographic images. Mach Vis Appl 2012; 23 (2): 299-311
8. Harshaw C, Alberts JR. Group and individual regulation of physiology and behavior: A behavioral, thermographic, and acoustic study of mouse development. Physiol Behav 2012; 106 (5): 670-682
9. Rudaya, AY, Steiner AA, Robbins JR, et al. Thermoregula-tory responses to lipopolysaccharide in the mouse: dependence on the dose and ambient temperature. Am J Physiol Regul Integr Comp Physiol 2005; 289 (5): 1244-1252
10. Henderson JM. Gastrointestinal pathophysiology. Moscow: Binom, 2010; 272 s.

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