Saratov JOURNAL of Medical and Scientific Research

Physiology and Pathophysiology

The relation of carbohydrate metabolism disorders and markers of endothelial dysfunction in animals with absolute insulin defciency at biostimulation by autotransplantation of the skin fap

Year: 2019, volume 15 Issue: №2 Pages: 379-38
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Popykhova E.B., Ivanov A.N., Stepanova T.V., Pronina E.A., Lagutina D.D.
Organization: Saratov State Medical University
Summary:

The purpose of this work is to study the relationship of carbohydrate metabolism disorders and markers of endo-thelial dysfunction in animals with absolute insulin defciency at biostimulation by autotransplantation of the skin fap. Material and Methods. The study was conducted on 60 male white outbred rats, which were divided into: an intact control group, a two comparative and an experimental group. In animals of the comparison group, alloxan diabetes mellitus (DM) was caused, in rats from the experimental group, an autotransplantation of the skin fap was performed against the background of developed alloxan diabetes. The levels of glycated hemoglobin (HbA1c), glucose, C-reactive protein (CRP) and vascular endothelial growth factor (VEGF) were determined. Results. Autotransplantation of the skin fap causes a normalization of the glycemic profle in animals of the experimental group, and also reduces subclinical infammation caused by hyperglycemia and stabilizes the expression of VEGF.Diabetes causes an increase in HbA1c by 69 % and CRP by 11 %, while аutotransplantation of the skin fap leads to a decrease in the concentration of HbA1c by 28 % and CRP by 7 %. Conclusion. Autotransplantation of the skin fap reduces the risk of microcirculatory disorders due to hyperglycemia and chronic subclinical infammation.

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Endothelial dysfunction in burn injury

Year: 2019, volume 15 Issue: №2 Pages: 377-379
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Morrison V.V., Bozhedomov A.Yu., Morrison A.V.
Organization: Russian State Medical University, Saratov State Medical University
Summary:

Objective: to estimate the level of markers of endothelial dysfunction in burn injury of various severities. Material and Methods. 57 patients were included in the study. The level of vascular endothelial growth factor (VEGF), endothe-lin, nitric oxide metabolites (NO), and the amount of circulating desquamated endothelial cells were determined in the dynamics of burn injury development. Results. In burn injury there was an increased amount of endothelial dysfunction markers in blood, which is highly expressed on the 7-15th day. Moreover on the 7th day in case of severe and extremely severe burn injury the level of VEGF was increased over 50 times while the amount of endothelin, nitric oxide metabolites and circulating desquamated endothelial cells becomes higher in 3–5 times. Conclusion. The research outcomes have proved the damage of endothelial lining in burn injury.

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Pathophysiological evaluation of common neonatal factors of the pathogenesis of hemolytic anemia and infammation

Year: 2019, volume 15 Issue: №2 Pages: 372-376
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Geraskin V.A., Potemina T.E., Geraskin I.V., Komlev D.Yu.
Organization: Privolzhskiy Research Medical University
Summary:

Objective: to determine the damage of blood elements as tissue damage — to study hemolytic anemia from the position of infammation. Material and Methods. Clinical and laboratory data of 187 neonatal patients were included in the examination group. The dynamics of blood erythrocyte indices was studied taking into account the dependence on age, anthropometric parameters, the severity of diseases and the intensity of oxygenotherapy. The composition and features of oxidative modifcations of lipid structures of biological membranes of erythrocytes were investigated. Results. The values of the elements of alteration in the pathogenesis of hemolysis were determined. Changes of erythrocyte membranes in oxidative hemolysis were investigated. The review and comparison of the information contained in the classical and periodicals on pathological physiology, Hematology. Conclusion. The study damage to the blood elements as tissue damage allows you to apply for the study of hemolytic anemias the concept of development of infammation.

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Composite nanomaterials on the basis of magnetic nanoparticles for theranostic application

Year: 2019, volume 15 Issue: №2 Pages: 368-371
Heading: Physiology and Pathophysiology Article type: Review
Authors: Bucharskaya A.B.
Organization: Saratov State Medical University
Summary:

Literature review is devoted to the use of composite nanomaterials based on magnetic nanoparticles for the diagnosis and treatment of various diseases.

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Intense light deprivation as a factor in stress disorders of behavioral reactions and cognitive functions in the experiment

Year: 2019, volume 15 Issue: №2 Pages: 363-368
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Antipova O.N., Ivanov A.N., Zlobina O.V., Kirichuk V.F., Surovtseva K.A., Ankina V.D., Bondar G.D., Zenkina T.M., Polyukova M.V.
Organization: Saratov State Medical University
Summary:

Objective: to study the efect of light desynchronosis on behavioral reactions and cognitive functions of white male rats. Material and Methods. The study was conducted on 36 intact non-pedigree white male rats, which were divided into 2 groups: experimental and control. Animals were exposed to artifcial illumination 24 hours a day provided by the fuorescent lamp of 60 kW capacity. The same animals of the experimental group on the 10th, 21st and 30th days were tested in the installations «Dark-light camera» and «Open feld». Results. On the 10th day of intensive light deprivation, the indicators refecting the research-orientation activity reached the maximum values, which indicated the development of stress damage to the animal organism. On the 21st day of the experiment, the

Keywords: pneumocysts
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Features of angiogenesis in skin tissue regeneration

Year: 2019, volume 15 Issue: №1 Pages: 104-107
Heading: Physiology and Pathophysiology Article type: Review
Authors: Pronina Е.А., Stepanova T.V., Kiriyazi T.S., Maslyakov V.V., Ivanov A.N.
Organization: Saratov Medical University "Reaviz", Saratov State Medical University, Saratov State Technical University n.a. Yu.A. Gagarin
Summary:

Restoration and regeneration of tissues are complex processes involving a large number of signaling mechanisms. In this study possible mechanisms of angiogenesis during tissue regeneration are presented. One of these mechanisms is the creation of a new capillary layer by angiogenesis. Previously it was believed that high and rapid levels of capillary growth are necessary for optimal healing of tissues. But several studies have demonstrated that a period of vascular regression occurs after the growth of blood vessels in a wound, and the number of the newly formed vessels decreases until the number of the blood vessels is equal to the density of the vascular network in the intact tissue. Reduced inflammation and angiogenesis are features of optimal healing.

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Comparative characteristics of microcirculatory changes in white rats during subcutaneous implantation tests of polycaprolactone scaffolds containing vaterite and hydroxyapatite

Year: 2019, volume 15 Issue: №1 Pages: 98-103
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Ivanov A.N., Kurtukova М.О., Chibrikova Yu.A., Kustodov S.V., Tyapkina D.A., Bugaeva I.O., Norkin IA.
Organization: Saratov State Medical University
Summary:

Objective: comparative evaluation of skin microcirculatory functional changes over the implantation area of polycap-rolactone scaffolds with mineral carriers: vaterite and hydroxyapatite. Material and Methods. The experimental study was performed on 43 rats divided into three groups: two test groups and one negative control group. Polycaprolactone PCL-scaffolds containing ovalbumine and of no biocompatibility were implanted into the rats of negative control group (n=15). The implantation of PCL- scaffolds containing vaterite under their skin was performed on rats of the first test group, and animals of the second test group were implanted with PCL-scaffolds containing hydroxyapatite. The method of laser Doppler flowmetry was used to study the microcirculatory flow. Results. The constant increase in the perfusion index has been observed in the area of non-biocompatibile scaffolds implantation. Thechanges in local skin blood flow modulation have been also detected. The transient character of local microcirculatory responses with total normalization of skin perfusion and its mechanisms has been noticed by the 21st day of the trial following PCL-scaffolds with vaterite and hydroxyapatite implantation. Conclusion. The dynamic monitoring has allowed finding that mineralization of PCL-scaffolds with vaterite as well as hydroxyapatite leads to no prominent changes in skin perfusion at subcutaneous implantation tests on white rats suggesting the biocompatibility of these scaffold types.

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Influence of drinking behavior on the content of alpha-amylase of oral fluid in students with and without predictor of inflammation on the background of educational stress

Year: 2019, volume 15 Issue: №1 Pages: 9-13
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Baisheva GM, Melnik KN.
Organization: Togliatti Clinical Hospital №5, Samara State Medical University
Summary:

 The aim: to study the content of a-amylase in oral fluid in healthy young people (mean age 18 years), depending on the presence of a predictor of inflammatory diseases of the oral cavity in physiologically adjusted drinking behavior against the background of educational stress. Material and Methods. For three months, we studied three groups: the main group (30 people, consumed water 30-35 ml per 1 kg of body weight treated with the electromagnetic device), a placebo group (30 people used water 30-35 ml per 1 kg of body weight, but consumed water with a placebo device that does not have an electromagnetic base) and a control one (25 people who do not change their water drinking behavior). To identify the features of sAA oscillations, all groups were divided into subpopulations with predictors of inflammatory diseases of the oral cavity (IL-1(3>212 pg/ml) and without such status (IL-1(3<212 pg/ml). The level of a-amylase activity was determined by a colorimetric method on Cobas C111 automated equipment (Roche), the concentration of IL-1J3 was detected by ELISA on an Infinity F50 photometer (Tecan). Results. Secretory alpha-amylase activity by the end of the study decreased in the main group; in the placebo and control groups, baseline values were exceeded. In the subpopulations with predictors of inflammatory dental diseases (IL-1(3<212 pg/ml) of the main group, the sAA activity decreased by 2.5 times; in the subpopulations without predicates of inflammatory changes (IL-1(3 <212 pg/ml), a significant decrease in enzyme activity in the main group was observed and an increase in the control. The percentage of sympathotonics at the end of the experiment in the main group was lower than in the placebo and control groups. Conclusion. A relation was found between a decrease in the level of a-amylase activity in the oral fluid and corrected water drinking behavior in a subgroup of people without predictors of inflammatory diseases of the oral cavity, using water in an amount of at least 30-35 ml/kg body weight that was purified by an electromagnetic method.

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The estimation of biocompatibility of polycaprolactone matrices mineralized by vat-erite in subcutaneous implantation tests in white rats

Year: 2018, volume 14 Issue: №3 Pages: 451-456
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Ivanov A.N., Kurtukova М.О., Kozadaev M.N., Tyapkina D.A., Kustodov S.V., Saveleva M.S., Bugaeva I.O., Parakhonsky B.V., Galashina E.A., Gladkova E.V., Norkin I.A.
Organization: Saratov National Research University n.a. N. G. Chernyshevsky
Summary:

Aim: to estimate biocompatibility of matrices produced from polycaprolactone (PCL) and mineralized by vaterite (CaC03) by studying local and systemic manifestations of inflammatory reaction in subcutaneous implantation tests in white rats. Material and Methods. The experiment was conducted on 40 rats divided into four equal groups: control, comparison (rats with imitation of implantation), negative control (rats with implanted non-biocompatible matrices) and experimental group, comprised of animals with implanted PCL/CaC03-matrices. Local inflammatory manifestations were analyzed by morphological assay of implantation area tissues. Systemic inflammatory manifestations were estimated by TNF-a concentration and interleukin-lp (IL-1) in blood serum by ELISA. Results. The changes in cellular population content demonstrate that a PCL/CaC03-matriceonthe21 day after the implantation to rats is evenly colonizing by fibroblast cells and vascularizing. This type of matrices does not provoke intense inflammatory reaction seen in negative control animals and accompanied by systemic manifestations such as statistically significant rise in TNF-a and IL-1 concentrations. Conclusion. The data obtained in the study proving the biocompatibility of PLC/CaC03-scaffolds experimentally substantiate the potential for their use in tissue engineering.

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The study on the refraction of morphofunctional changes in kidneys of white male rats with experimental light desynchronosis

Year: 2018, volume 14 Issue: №3 Pages: 363-367
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Zlobina O.V., Ivanov A.N., Antonova V.M., Milashevskaya T.V., Bugaeva I.O.
Organization: Saratov State Medical University
Summary:

The goal is to study the morphofunctional changes in the kidneys under the influence of light desynchronosis, an analysis of the degree of reversibility of these changes. Material and Methods. The study was carried out in two stages on 72 white male rats. In the first series of experiments, animals underwent round-the-clock light exposure for 10 and 21 days. In the second series after round-the-clock exposure to light for 10 and 21 days, the beings were exposed to natural light for 21 days. The animals of the control group were kept in a natural light regime. Morphological and morphometric analysis of histological sections of kidneys stained with hematoxylin and eosin was performed. Results. It was found that on the 10th day of the experiment, cellular infiltration of the cortex occurs, and the edema of the interstitial tissue develops. These changes progress to the 21st day of the experiment and are combined with a significant decrease in the area of the glomerular capillary network and expansion of the lumen of the capsule of the glomerulus of cortical nephrons. When assessing the long-term consequences after 10-day illumination, the diameter of the renal corpus is reduced along the long and short axis. The changes registered on the 21st day do not reach the control values, which indicate that they are irreversible. Conclusion. The revealed structural and functional changes in the kidney indicate a negative effect of light desynchronosis: 10-day lighting leads to a breakdown of compensatory possibilities and the development of distant morphological changes; long-term light exposure (within 21 days) leads to the development of pronounced irreversible structural disorders.

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