Saratov JOURNAL of Medical and Scientific Research

Physiology and Pathophysiology

The estimation of biocompatibility of polycaprolactone matrices mineralized by vat-erite in subcutaneous implantation tests in white rats

Year: 2018, volume 14 Issue: №3 Pages: 451-456
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Ivanov A.N., Kurtukova М.О., Kozadaev M.N., Tyapkina D.A., Kustodov S.V., Saveleva M.S., Bugaeva I.O., Parakhonsky B.V., Galashina E.A., Gladkova E.V., Norkin I.A.
Organization: Saratov National Research University n.a. N. G. Chernyshevsky
Summary:

Aim: to estimate biocompatibility of matrices produced from polycaprolactone (PCL) and mineralized by vaterite (CaC03) by studying local and systemic manifestations of inflammatory reaction in subcutaneous implantation tests in white rats. Material and Methods. The experiment was conducted on 40 rats divided into four equal groups: control, comparison (rats with imitation of implantation), negative control (rats with implanted non-biocompatible matrices) and experimental group, comprised of animals with implanted PCL/CaC03-matrices. Local inflammatory manifestations were analyzed by morphological assay of implantation area tissues. Systemic inflammatory manifestations were estimated by TNF-a concentration and interleukin-lp (IL-1) in blood serum by ELISA. Results. The changes in cellular population content demonstrate that a PCL/CaC03-matriceonthe21 day after the implantation to rats is evenly colonizing by fibroblast cells and vascularizing. This type of matrices does not provoke intense inflammatory reaction seen in negative control animals and accompanied by systemic manifestations such as statistically significant rise in TNF-a and IL-1 concentrations. Conclusion. The data obtained in the study proving the biocompatibility of PLC/CaC03-scaffolds experimentally substantiate the potential for their use in tissue engineering.

AttachmentSize
2018_3_451-456.pdf306.1 KB

The study on the refraction of morphofunctional changes in kidneys of white male rats with experimental light desynchronosis

Year: 2018, volume 14 Issue: №3 Pages: 363-367
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Zlobina O.V., Ivanov A.N., Antonova V.M., Milashevskaya T.V., Bugaeva I.O.
Organization: Saratov State Medical University
Summary:

The goal is to study the morphofunctional changes in the kidneys under the influence of light desynchronosis, an analysis of the degree of reversibility of these changes. Material and Methods. The study was carried out in two stages on 72 white male rats. In the first series of experiments, animals underwent round-the-clock light exposure for 10 and 21 days. In the second series after round-the-clock exposure to light for 10 and 21 days, the beings were exposed to natural light for 21 days. The animals of the control group were kept in a natural light regime. Morphological and morphometric analysis of histological sections of kidneys stained with hematoxylin and eosin was performed. Results. It was found that on the 10th day of the experiment, cellular infiltration of the cortex occurs, and the edema of the interstitial tissue develops. These changes progress to the 21st day of the experiment and are combined with a significant decrease in the area of the glomerular capillary network and expansion of the lumen of the capsule of the glomerulus of cortical nephrons. When assessing the long-term consequences after 10-day illumination, the diameter of the renal corpus is reduced along the long and short axis. The changes registered on the 21st day do not reach the control values, which indicate that they are irreversible. Conclusion. The revealed structural and functional changes in the kidney indicate a negative effect of light desynchronosis: 10-day lighting leads to a breakdown of compensatory possibilities and the development of distant morphological changes; long-term light exposure (within 21 days) leads to the development of pronounced irreversible structural disorders.

AttachmentSize
2018_3_363-367.pdf516.04 KB

Gender and age peculiarities of aortic pressure in healthy adolescents

Year: 2018, volume 14 Issue: №2 Pages: 197-200
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Sheveleva A.M.
Organization: Volgograd State Medical University
Summary:

Aim: to reveal gender and age peculiarities of daily dynamics of systolic aortic pressure (SBPao), diastolic aortic pressure (DBPao), mean hemodynamic aortic pressure (MBPao) and pulse aortic pressure (PPao) in healthy adolescents. Material and Methods. Ambulatory blood pressure monitoring was performed in 354 healthy adolescents (184 girls, 170 boys) aged 12-17 years. Results. Daytime, nighttime and diurnal levels of SBPao and PPao were significantly lower in girls, than that of boys. Boys had significantly lower daytime and diurnal levels of DBPao than girls. Nighttime level of MBPao was significantly higher in boys than in girls. Growth positively correlated with daytime, nighttime and diurnal levels of SBPao (r=0.3, r=0.4and r=0.4 respectively) in boys 12-17 years old. Correlation analysis in girls 12-17 years showed a weak positive relationship between growth and nighttime, daytime and diurnal levels of SBPao (r=0.14, r=0.2 and r=0.14 respectively). Conclusion. The observed differences between age subgroups could be associated with the different velocity of pubertal development in the examined adolescents, as well as with the different role of hormonal and neuronal regulation mechanisms in the formation of aortic pressure level and its diurnal dynamics.

AttachmentSize
2018_2_197-200.pdf244.64 KB

Peculiarities of microcirculatory reactions after subcutaneous implantation of polycaprolactone matrices mineralized by vaterite

Year: 2018, volume 14 Issue: №1 Pages: 35-41
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Norkin I.A., Ivanov A.N., Kurtukova M.O., Savelyeva M.S., Martyukova A.V., Gorin D.A., Parakhonsky B.V.
Organization: Saratov State Medical University, Saratov state university, Skolkovo Institute of Science and Technology
Summary:

The aim is to assess skin microcirculation changes arising during subcutaneous implantation of polycaprolactone scaffolds mineralized by vaterite. Material and Methods. The experiment was carried out on 30 albino rats divided into two groups: a negative control group and an experimental one. We implanted polycaprolactone scaffold with exhausted foreign protein subcutaneously into rats of the negative control group. We implanted polycaprolactone matrix mineralized by vaterite subcutaneously into the animals of the experimental group. Research methods include laser Doppler flowmetry and morphological examination of the tissues of the matrix implantation area. Results. Changes of skin microcirculation over the matrix allocation area correspond to the morphological pattern of tissue reactions. Biocompat-ibility disorders take the form of inflammation in the scaffold implantation area that is accompanied by stable perfusion rise associated with local bloodstream modulation changes. We did not observe inflammation signs in the surrounding scaffold tissues during implantation of polycaprolactone matrices mineralized by vaterite. Besides local microcircula-tory reactions possess a transient character disappearing completely by the 21st day after the implantation. Conclusion. Complex of the given functional and morphological studies allow us to ascertain high-grade biocompatibility of polycaprolactone matrices mineralized by vaterite thus giving rise to prospect of their use for tissue regeneration stimulation.

AttachmentSize
2018_1_035-041.pdf324.92 KB

The modified assessment technique of the condition of lungs at the experimental animals at extreme exposures

Summary:

Purpose: to develop a modified method of measuring a set of biophysical indices of the lungs elaborated, which allows to assess the extent of their lesions from mild, moderate to severe. This technique allows for a short time to conduct a rapid assessment of the lungs of a large number of experimental animals of different species under varying extreme influences for the subsequent extrapolation of these data to humans. Material and Methods. The study was conducted using Chinchilla rabbits weighing 2.5-3.0 kg, and sheep weighing 25-35 kg. Lung mass and volume were defined, and values of biophysical indices were calculated: specific density of air-filled and collapsed lungs; their ratio — ALPHA index; residual volume. Results. The values of main lung biophysical indices of experimental animals in norm were received. The scale of lung condition estimate and degree of injury of experimental animals as compared with the control group was developed. Conclusion. The given technique of rapid estimate of the lung condition according to biophysical indices allows to give a rapid and objective assessment of significant lung injuries in experimental animals. Easy realization and availability of necessary equipment enable to use this technique in the studies of experimental lung pathology.

AttachmentSize
2017_04-1_907-912.pdf549.39 KB

Investigation of the relationship between changes in thermographic and flowmetric parameters of skin peripheral hemodynamics in laboratory rats

Year: 2017, volume 13 Issue: №4 Pages: 901-907
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Datsenko A.V., Fomina T.V., Dyoshin I.A., Kazmin V.I.
Organization: State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency
Summary:

Purpose: to determine the quantitative patterns and gradations of the degree of manifestation of changes in IRT indicators, depending on LDF data, corresponding to different conditions and peripheral blood flow disorders in the skin of laboratory rats. Material and Methods. Microcirculation of blood in bio-objects in background studies and immediately after the experimental modeling of hypobaric hypoxia was determined with the help of LDF, while simultaneously performing remote dynamic infrared thermography of the rats'tail skin. A comparison of LDF and IRT data was carried out using statistical methods of correlation-regression analysis. Results. The decrease in tissue blood flow was accompanied by a decrease in temperature, with an increase in perfusion of blood along the microcirculation pathways, the temperature of the skin surface increased. The quantitative criteria of experimental diagnostics in the form of boundary values and ranges of temperature indicators for an estimation of a different degree of severity of violations of the microcirculation of blood (peripheral hemodynamics) are determined. With a decrease or increase in tissue blood flow to 10, 10-25, 25-40 and more than 40% of the baseline (background control), the negative or positive skin surface temperature increase was up to 0.6, 0.6-1.2, 1.2-1.8 and more than 1.8°C respectively. Conclusion. Results of thermal imaging can be used as a criterion for predictive assessment of the state and changes in cutaneous peripheral blood flow in laboratory rats.

AttachmentSize
2017_04-1_901-907.pdf615.49 KB

Exotoxin A Pseudomonas aeruginosa influence on working efficiency of white rats

Year: 2017, volume 13 Issue: №3 Pages: 472-474
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Morrison V.V., Popovich V.I., Vasyutina V.O., Morrison A.V.
Organization: Central Scientific Research Experimental Institute 33, Saratov State Medical University
Summary:

Objective: to study exotoxin A Pseudomonas aeruginosa influence on physical and mental efficiency of white rats. Material and Methods. The experiments were carried out on white rats following intraperitoneal injections of exotoxin A Pseudomonas of different doses (0.1,0.5 and 1.0 LD50). Physical and mental efficiency of white rats was determined by the scheme of "Machfs camera". Results. Introducing exotoxin A Pseudomonas of 1 LD50 there was conditioned response damage in 20% of animals. The amount of cases of physical incapacity introducing highly effective doses of exotoxin A Pseudomonas overcomes the amount of cases of reaction destruction (44%). Conclusion. Exotoxin A Pseudomonas aeruginosa does not reveal a selective effect on the central nervous system and significant influence on physical efficiency of white rats.

AttachmentSize
2017_03_472-474.pdf349.63 KB

Different reaction of core histones H2A and H2B to the red laser radiation

Year: 2017, volume 13 Issue: №3 Pages: 469-472
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Brill G.E., Egorova A.V., Bugaeva I.O., Ushakova O.V., Matyushkina O.L.
Organization: Saratov State Medical University, Yuri Gagarin State Technical University of Saratov
Summary:

Aim: to investigate the influence of red laser irradiation on the processes of self-assembly of core histones H2A and H2B. Material and Methods. Solutions of human histone proteins were used in the work. Self-assembly was studied by the method of wedge dehydration. Image facies analysis consisted in their qualitative characterization and calculation of quantitative indicators with subsequent statistical processing. Results. It was established that linearly polarized laser light of the red region of the spectrum (A=660 nm, 1 J/cm2) significantly modifies the process of self-assembly of core histone H2B, while the structure of the facies of H2A histone changing to a lesser extent. Conclusion. Red laser radiation influences on the on the processes of self-assembly of core histones H2A and H2B. There is a differential sensitivity of different classes of histones to laser action. Histone proteins used in the experiments are present in the form of aqueous salt solutions. Red light realizes the effect seems to be due to the formation of singlet oxygen by direct laser excitation of molecular oxygen.

AttachmentSize
2017_03_469-472.pdf413.56 KB

Systemic inflammatory response and cytokine profile at burn injury in dynamics

Year: 2017, volume 13 Issue: №2 Pages: 229-232
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Morrison V.V., Bozhedomov A.Yu., Simonyan M.A., Morrison A.V.
Organization: Leningrad Regional Clinical Hospital, Saratov State Medical University
Summary:

The aim of the article is to study the dynamics of indices of systemic inflammation in correlation with the following factors: the outcome of the disease, disturbances in proinflammatory cytokines rate and vascular endothelial dysfunction markers rate at burn injury in dynamics. Material and Methods. 152 patients with burn injuries over 30 units by Frank's index were under the study. The research on the level of interleukins, endothelial vessels growth factor, macrophage chemotactic protein, level of endothelin and C-reactive protein was performed with quantitative immuno-enzymatic detection. The amount of circulating of desquamated endothelial cells was accounted by phase-contrast microscopy. Results. Proinflammatory cytokines rate and vascular endothelial dysfunction markers rate significantly increase reaching their maximum indices to the 7-15th day of burn injury and indicating the expressiveness of systemic inflammatory response syndrome. Conclusion. The received data have become important in the determination of increased number of criteria of systemic inflammatory response syndrome depending on the outcomes of the burn injury and endothelial dysfunction development.

AttachmentSize
2017_02_229-232.pdf316.73 KB

Acousto-optical method of blood typing: the comparison of application of anti-A and anti-B monoclonal antibodies with standard hemagglutinating sera

Year: 2017, volume 13 Issue: №1 Pages: 022-028
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Doubrovski V.A., Medvedeva M.F.
Organization: Saratov State Medical University
Summary:

The aim of the article is a comparison of resolution of the acousto-optical method for blood typing for two types of reagents: monoclonal antibodies and standard hemagglutinating sera. Materials and Methods: The influence of the concentrations of reagents (monoclonal antibodies and standard hemagglutinating sera), of blood sample, which is to be tested, as well as of the duration of the ultrasonic action on the biological object upon the resolution of acousto-optical method were investigated. The peculiarity of this work is the application of digital photo images processing by pixel analysis previously proposed by the authors. Results: The optimal experimental conditions to obtain maximum of the resolution of the acousto-optical method were found, it creates the prerequisites for a reliable blood typing. Conclusions: The present paper is a further step in the development of acousto-optical method for determining human blood groups.

AttachmentSize
2017_01_022-028.pdf534.62 KB