Saratov JOURNAL of Medical and Scientific Research

New method of investigation of urine microflora in mice after irradiation

Year: 2013, volume 9 Issue: №4 Pages: 890-892
Heading: radiation medicine Article type: Short message
Authors: Ivanov A.A., Shalnova G.A., Maltsev V.N., Ulanova A.M., Stavrakova N.M., Bulynina T.M.
Organization: State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency
Summary:

For investigation of urine microflora in mice we offered to use paper disks. This new method was tested in finding of infection on the mice (CBAxC57BI) F1 after total body v-irradiation at 6,5 Gr (LD80/30). The paper disks were impreg-
nated with one drop of urine, placed on surface Endo media, incubate in thermostat (37°C) in during 1-2 days and numbers of positive crops (Esch. coli, Proteus, Enterococcus, were indicated. It was shown that after irradiation the numbers of positive probes increased. The test displayed that the method is easy, economic and demonstrative.

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