Saratov JOURNAL of Medical and Scientific Research

radiation medicine

Elaborating of a cryoconservation protocol of blood vessels for the tissue-specific matrices

Year: 2017, volume 13 Issue: №4 Pages: 891-900
Heading: radiation medicine Article type: Original article
Authors: Lauk-Dubitsky S.E., Astrelina Т.А., Brumberg V.A., Nikitina V.A., Suchkova Yu.B., Usupzhanova D.Yu., Brunchukov V.A., Rastorgueva A.A., Lomonosova Е.Е., Kobzeva I.V., Makhova A.E., Karaseva T.V., Bushmanov A.Yu., Samoilov A.S.
Organization: State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency
Summary:

Purpose: to create a protocol for complex cryoconservation of blood vessels in polydimethylsiloxane to create tissue-specific matrices. Material and Methods. The material ofthe study was the iliac arteries from postmortem donors, seized in the multi-organ donation, rejected for unclaimed and disposed of later. Polydimethylsiloxane was used as a coolant (PDMS) with a viscosity of 5-25 centistokes (Spectroplast, Russia). Bench testing of cooling and heating ofthe blood vessel in PDMS, testing of heating and cooling of PDMS up to operating temperatures (-80°C) in different ways and variants of its thermal insulation, assessment of different cooling rates ofthe vessel during cryoconservation, modeling of 3 types of working chambers for cryoconservation, sterilization and defrosting of up to 10 vessels, modeling of cryoconservation and thawing of vessels in PDMS under different conditions, selection of substrates from silicone and plastic for uniform fixation of vessels which are of size or size in the working chambers or without them. Results. The developed protocol allows cryopreserved from 1 to 10 or more vessels of all basic sizes both immediately after removal and after transportation to the laboratory, to vary the cooling rate from 1.5 to 300°C/min and above, to be used with different levels of laboratory equipment, as with liquid nitrogen, dry ice, and only with a medical freezer. Conclusion. The developed protocol allows to apply it in cases of complex cryoconservation of blood vessels to create tissue-specific matrices and can be recommended for preclinical testing.

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Qualitative and quantitative comparisons of bone PET-imaging using 68Ga-oxabiphor and Na18F

Year: 2017, volume 13 Issue: №4 Pages: 886-891
Heading: radiation medicine Article type: Original article
Authors: Lunyov A.S., Clement'eva О.Е., Lunyova К.А., Zhukova M.V., Malysheva А.О.
Organization: State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency
Summary:

The aim of the study is to compare qualitative and quantitative characteristics of the experimental mice bone pathology using of Na18F and prospective radiotracer"Oxabigal, 68Ga" (complex of gallium-68 with oxa-bys-ethylenenitrile tetra (methylene phosphonic acid). Material and Methods. 30 nonlinear mice-female with the model of bone pathology were used in the experiment. Na18F and "Oxabigal, 68Ga" were injected to animals (i.v). Then mice were scanned using PET-imaging with subsequent calculation of pharmacokinetics parameters. Results. The intravenous injection of two radiotracers enabled to visualize of simulated bone pathology using PET. Conclusion. The experimental pathological foci were able to visualize within the first hour after i.v using of the "Oxabigal, 68Ga" and Na18F. There is a statistically significant difference between blood accumulation of radiopharmaceuticals labeled different radionuclides. However the difference doesn't matter for positive results we have got. 68Ga-labeled bone-seeking radiopharmaceuticals and its generator producing method are important supplement for independent choice of using different radiotracers for PET-imaging (such as Na18F) of bone pathology.

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Risk of cataract in the context of acute and chronic exposure

Year: 2016, volume 12 Issue: №4 Pages: 678-684
Heading: radiation medicine Article type: Original article
Authors: Tukov A.R., Shafransky I.L., Kapitonova N.V., Prokhorova O.N., Samoilov A.S.
Organization: State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency
Summary:

Purpose: estimation of the risk of cataract using doses of different types of radiation. Material and methods. The study is carried out using the information database of the NP, recovery workers of the accident at the Chernobyl NP. Professional exposure and dose received during 30 km zone were used to calculate the risk. Results. The study shows the use of one of their parts of the total radiation dose of man, leads to obtaining of different levels of the risk of disease. Conclusion. Only use of a total radiation dose can lead to obtaining of the correct results of evaluating the risk of the emergence of the radiation- induced diseases.

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FISH-method: technique of cytogenetic retrospective dose evaluation

Summary:

The purpose of this work was to submit the review of data of the scientific literature devoted to a FISH method as a way of cytogenetic retrospective dose evaluation. Problems of cytogenetic retrospective assessment of a dose in general; molecular and biological bases of a FISH-method; types of chromosome aberrations identified by means of a FISH-method; the principles of creation of dose curves for the FISH-registered chromosome translocations have been considered. The conclusion is drawn on need of attraction of three-color and multi-color variants of this technique to retrospective dose evaluation.

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Application evaluation of a polydimethylsiloxane low-viscosity for cryopreservation and radiation sterilization of human cadaveric vascular allografts.

Year: 2016, volume 12 Issue: №4 Pages: 662-670
Heading: radiation medicine Article type: Original article
Authors: Lauk-Dubitsky S.E., Astrelina Т.А., Brumberg V.A., Fedyunin A.A., Kamyshnikov O.Yu., Vostrukhin S.V., Gorde'yev A.V., Paklina O.V., Kobzeva I.V., Nikitina V.A., Suchkova Yu.B., Usupzhanova D.Yu., Brunchukov V.A., Karaseva T.V., Bushmanov A.Yu., Samoilov A.S.
Organization: State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency, City Clinical Hospital n.a. S.P. Botkin
Summary:

Aim: to investigate potential cryo- and radioprotective properties of a polydimethylsiloxane of low viscosity to provide safe and reliable cryopreservation and radiation sterilization of human cadaveric vascular allografts. Material and methods. A consecutive experimental set-up was carried out to establish polydimethylsiloxane (further — PDMS) protective endocellular properties. For this purpose a high-densitymesenchymal stem cells suspension was cryopreserved with 10% DMSO solution; PDMS exocellular protective properties were assessed by cyclic cryoconservation of ^femoral artery segments, while PDMS potential radioprotectiveproperties were assessed by gamma irradiation — induced sterilization of 3 cryopreserved femoral artery segments with gross length about 3cm, and for control grafts a slightly changed protocol with glycerin was used. In this study the dynamic viscosity of all cryopreserved vessels was analyzed with using specially designed experimental device, also electron scanning microscopy with lanthanide cells staining and routine hematoxylin — eosin cell staining were applied. Results. We have demonstrated a prominentexocellular protective effect of PDMS confirmed by a SEM and histological results, and also its mediated radioprotective effect, in particular because of its safer preliminary cryopreservation procedure. Conclusion. Use of PDMS for a cryopreservation and gamma sterilization showed its rationality, however requires further modification of protocols and additional researches.

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The question of a 'New Era in the Low Dose Radiation Epidemiology' approach

Year: 2016, volume 12 Issue: №4 Pages: 654-662
Heading: radiation medicine Article type: Review
Authors: Koterov A.N., Ushenkova L.N., Biryukov А.Р., Samoilov A.S.
Organization: State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency
Summary:

The historical and recent years data on cancer and/or leukemia rate increasing after exposure of people in low dose (up to 100 mGy) radiation with a low LET, attributiveness of effects and the possibility of their experimental confirmation were considered. Previously, information about the carcinogenic effects of low doses was not clearly interpreted due to the presence of uncertainty, biases and confounders. The biological mechanism was also absent. In the last 5-7 years the situation has changed dramatically: more significant data were obtained: irradiation at computed tomography in dentistry, for residents of a high natural background radiation, and others. Simultaneously it was obtained radiobio-logical data on increased DNA double strand breaks level after exposure to doses from few milligrays and it can be considered as a possible molecular mechanism of these effects. As a result, was declared 'New Era in the Low Dose Radiation Epidemiology' (Kitahara С M., et al., 2015), which can lead to costs in terms of tightening of radiation risks and to fear of medical exposure. The conducted in the review analysis of recent epidemiological and radiobiological evidence suggests, however, that there is no unambiguous evidence of attributed to radiation carcinogenic effects, identified during the 'New Epidemiology of Low Doses', and there is no a proven molecular mechanism that could provide biological plausibility of such effects.

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Proposals on harmonization of the list of the diseases connected with radiation effects as the result of the accident on the CNPP

Year: 2016, volume 12 Issue: №4 Pages: 651-654
Heading: radiation medicine Article type: Author's opinion
Authors: Bushmanov A.Yu., Biryukov А.Р., Korovkina Е.Р., Kretov A.S., Kapitonova N.V.
Organization: State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency
Summary:

The lists of the diseases connected with radiation effects existing now at the level not only Russia and Belarus, but also at the Union State in general can be enhanced using cumulative scientific data. There are possible variants, as exceptions in the list of separate nosologies, reasons of which are rather caused not by radiation factors, and as introduction of diseases which radiation nature is confirmed by long-term researches. The conducted researches showed that creation of the single list of diseases within the Union State is a really question solving of which will demand not only significant scientific researches, but also serious legislative initiatives.

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Radiation risks: triumph of the ideal or escape from reality?

Summary:

Biological effects of low doses of radiation action and especially the problem of quantifying are still the subject of much debate and polar opinions about their hazards for a human and the environment. Nevertheless, the use of risk assessments under the control of the state of health of populations exposed is not widespread in the medical community. Does this indicate the need for revision and improvement of the radiation-epidemiological analysis techniques? Years of experience of the Federal Medical and Biophysical Center n.a. A. I. Burnazyan enable us to answerthis question from the standpoint of accumulated unique knowledge and methodologies by which managed to develop its own approach to optimize a core set of questions relating to the problems of long-term radiological effects of low doses.

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Effects of the vaccine "Grippol" on resistance of mice after irradiation by protons

Year: 2015, volume 11 Issue: №4 Pages: 656-658
Heading: radiation medicine Article type: Short message
Authors: Ivanov А.А., Abrosimova A.N., Bulinina Т.М.
Organization: State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency
Summary:

Aim: to investigate the effect of the vaccine "Grippol" on radioresistance with respect to proton irradiation. Material and methods. The effect of the vaccine "Grippol" the survival of the animals was studied in mice CBAxC57BI F1, which was immunized for 10 days prior to proton irradiation. Results. It is shown that proton irradiation causes the death of the animals at all doses tested: 9% at a dose of 7.0 Gy; 10% — 8.0 Gy and 33% after irradiation at a dose of 8.5 Gy, at the same time the survival rate after pre vaccination study groups was 100%. It seems appropriate to further study the impact of the vaccine "Grippol" at low doses of proton irradiation using appropriate test evaluation of hematological, im-munological, genetic, physiological and other factors. Conclusion. The vaccine "Grippol" introduction into mice before lethal proton irradiation increases survival of protected animals.

Effect of individual and group housing of mice on the level of radioresistance

Year: 2015, volume 11 Issue: №4 Pages: 653-656
Heading: radiation medicine Article type: Original article
Authors: Dorozhkina O.V., Bulynina Т.М., Ivanov А.А..
Organization: State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency
Summary:

Aim: to examine the effect of individual and group housing of mice on radioresistance. Material and methods. Effects of individual and group housing of mice on immunity and blood systems were studied on ICR (CD-1) and C57BI6 male mice before and after proton irradiation. Results. Group housing of intact animals resulted in a decline in the number of nucleated cells in the femur bone marrow and thymus mass. The irradiation with proton with energy of 171 MeV at a dose of 1 Gy causes a statistically significant greater reduction of the number of nucleated cells in the femur bone marrow in group-housed mice. A trend toward greater safety of the number of leukocytes in the peripheral blood and higher proliferative activity of bone marrow cells, as well as lower level of aberrant mitoses have been noted in individually-housed mice. Reduction processes in the recovery period of radiation sickness take place at a greater rate in group-housed mice. Conclusion. Group housing of male mice causes increased sensitivity of the blood and immunity systems to the effects of radiation and at the same time accelerates processes of radiation recovery.

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