Saratov JOURNAL of Medical and Scientific Research

Glukhova T.N.

Saratov State Medical University n.a. V.I. Razumovsky, Professor of Department of Obstetrics and Gynecology, DSc

Pregnancy and cervical intraepithelial neoplasia

Year: 2019, volume 15 Issue: №1 Pages: 19-23
Heading: Obstetrics and Gynecology Article type: Review
Authors: Yakovleva O.V., Glukhova T.N., Rogozhina I.E., Skupova I.N.
Organization: Saratov State Medical University
Summary:

The review of literature is devoted to the problem of cervical intraepithelial dysplasia in pregnant women. For the analysis, publications in the PubMed from 2013 till 2018 database were used.

AttachmentSize
2019_1_019-023.pdf266.17 KB

Molecular and cellular mechanisms of destabilization of membranes and prenatal discharge of amniotic fluid

Year: 2013, volume 9 Issue: №4 Pages: 644-648
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Mikhaylov А.V., Dyatlova L.I., Chesnokova N.P., Ponukalina Е.V., Glukhova T.N.
Organization: Perinatal Centre, Saratov , Saratov State Medical University
Summary:

Purpose: to establish the role of activation of lipid peroxidation and antioxidant deficiency of blood and amniotic fluid in the destabilization of membranes with early prenatal discharge of amniotic fluid. Material and methods. The analysis of lipid peroxidation products content in blood and amniotic fluid — diene conjugates (DC), malondialdehyde (MDA) and the quantitative determination of total peroxides (OxyStat), the estimation of the total antioxidant status (TAS), the activity of SOD and the content of ceruloplasmin in 20 pregnant with premature rupture of membranes in the gestation period of 22-34 weeks, were hospitalized at the Department of Pathology of Pregnancy of Saratov Perinatal Center in 2012. DC and MDA determination was carried out by conventional spectrophotometric methods of research, quantitative determination of peroxides (OxyStat) was carried out by enzyme immunoassay analyzer «Alfa Prime» («Meredith Diagnostics», 2008 using chemicals made by the company «Bender Medsystems»). The study of antioxidant status of serum and amniotic fluid was performed by enzyme immunoassay analyzer «Alfa Prime» («Meredith Diagnostics», England, 2008). Reagents («Bender Medsystems», Austria) were used to determine the total antioxidant status (TAS) and the SOD activity. To assess the content of ceruloplasmin reagent «Sentinel» (Italy) was used. Results. Indicators of lipid peroxidation in blood and amniotic fluid and anti-radical protection of biological membranes of cells and body fluids in the "mother-placenta-fetus" increased. Conclusion. Systemic activation of lipid peroxidation has been proved to be a
pathogenetic factor in the failure of membranes in prenatal discharge of amniotic fluid. The increase in the blood levels of lipid peroxidation products on the background of the activation of antioxidant system is considered as a prognostic sign of abortion.

AttachmentSize
2013-04_644-648.pdf322.34 KB

Changes in the cytokine profile of patients blood with premature rupture of amniotic membranes and their pathogenic significance

Year: 2013, volume 9 Issue: №2 Pages: 224-229
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Mikhaylov А.V., Dyatlova L.I., Chesnokova N.P., Ponukalina E.V., Glukhova T.N.
Organization: Saratov State Medical University, Perinatal Centre, Saratov
Summary:

Purpose: Pathogenetic study of new diagnostic and prognostic criteria for evaluation of pregnancy in terms of the cytokine profile of blood. Materials and methods: The analysis of anti-inflammatory and pro-inflammatory cytokines in the blood of 50 pregnant women with preterm rupture of membranes in gestation of 22-34 weeks, who were treated at the Department of Pathology in pregnancy of Saratov Perinatal Center in 2012, was performed. The method of enzyme linked immunosorbent assay using test systems «Vector— Best» (Novosibirsk, Russia). Results:.Clinical and laboratory examination revealed an increase levels of IL-1(3, IL-2, IL-6, IL-8, TNF-a, while reducing the content of IL-4, IL-10 in blood. Conclusion: Manifest signs of possibility of prenatal rupture of membranes and premature labour in 22-34 weeks gestation are combined with increasing of IL-1(3, IL-6, IL-8, TNF-a, IL-2 and with decreasing of IL-10, IL-4 level in the blood, which indicates the need for monitoring the content of these cytokines in the blood for the diagnosis and prognosis of pregnancy.

AttachmentSize
2013-02_224-229.pdf283.73 KB