Saratov JOURNAL of Medical and Scientific Research

Институт биохимии и физиологии растений и микроорганизмов РАН

Surface modification of orthodontic implants by nanocomposite coatings based on chitosan and metals

Year: 2013, volume 9 Issue: №3 Pages: 472-477
Heading: Stomatology Article type: Original article
Authors: Suetenkov D.Ye., Gorin D.A., Portnov S.A., Karagaychev A.L., Terentyuk G.S., Zakharevich A.M., Khlebtsov B.N., Firsova I.V.
Organization: Saratov State Medical University, Saratov state university, Институт биохимии и физиологии растений и микроорганизмов РАН
Summary:

Purpose: to study the properties of nanostructured coatings in orthodontic implants. Material and methods. Low and average molecular mass chitosan, 3-amynopropil-3-methoxysylan and suspensions of 5 nm and 10 nm gold nano-spheres stabilized with natrium citrate were used for nanocomposed surfaces. 2mg/ml polyethylenamin water solution was used for making the underlayer before putting polyion coverage using «POLYION-1M». The polyion covering dynamics was studied by polyquartz weighing method. Morphology of created layers was studied with atomic microscopy, elements were studied by secondary ion mass-spectrometry. Results. The best transmission among structures of chito-san/metals was showed by low molecular mass of chitosan and 8-1 Onm nanoparticles of metals. Analysis of roughness of surface shows that nanoparticles of gold make the most solid surface on 3-amynopropil-3-methoxysylan underlayer. Conclusion. The development of biocomparative materials in maxillofacial surgery is considered to be effective method of decreasing the risk of post-operative inflammatory complications by local antibacterial effect.

AttachmentSize
2013-03_472-477.pdf328.21 KB

Morphological changes in the organs of laboratory animals during the prolonged oral administration of gold nanoparticles

Year: 2013, volume 9 Issue: №2 Pages: 208-213
Heading: Macro- and Micromorphology Article type: Original article
Authors: Maslyakova G.N., Pakhomiy S. S., Bucharskaya А.В., Zlobina O.V., Navolokin N.A., Ponukalin A.N., Khlebtsov N.B., Khlebtsov B.N., Bogatyrev V.A.
Organization: Saratov State Medical University, Институт биохимии и физиологии растений и микроорганизмов РАН
Summary:

The purpose of the article is to assess the severity of the morphological changes in the internal organs of laboratory animals with prolonged oral administration of gold nanoparticles of different sizes. Material and methods: The experiment was carried out on 40 albino rats which were divided into 4 groups. Animals of the experimental group received oral gold nanoparticles by the appropriate scheme. Results: At comparing the morphological changes in the internal organs of laboratory animals it was established that the introduction of gold nanoparticles of different sizes leads to the same type of disturbances of morphological structure, the gold nanoparticles of 1-3 and 50 nm cause the most expressive changes. Conclusion: The most significant morphological modifications in the internal organs were caused by gold nanoparticles of 1-3 and 50 nm. It points out to the size-dependence of the effect of gold nanoparticles.

AttachmentSize
2013-02_208-213.pdf340.79 KB

Analysis of accumulation and biodistribution of gold nanoparticles in mesenteric lymph nodes by oral administration

Year: 2013, volume 9 Issue: №1 Pages: 17-20
Heading: Macro- and Micromorphology Article type: Original article
Authors: Zlobina O.V., Pakhomiy S. S., Bucharskaya A.B., Bugaeva I.O., Maslyakova G.N., Khlebtsov N.G., Khlebtsov B.N., Bogatyrev V.A.
Organization: Saratov State Medical University, Институт биохимии и физиологии растений и микроорганизмов РАН
Summary:

The aim of the article is to conduct analysis of accumulation and biodistribution of gold nanoparticles in the structural and functional areas of mesenteric lymph nodes of white rats. Particular attention is paid to the size and duration of oral administration. Material and Methods: Dark field microscopy with Leica DM 2500 microscope has been used to assess biodistribution of gold nanoparticle. The histochemical reaction of silver amplification with silver lactate by Danscher has been used to confirm gold nanoparticles identification. Results: It has been found that regardless of the duration of administration gold nanoparticles of 1-3 nm have not been detected by these methods in the lymph nodes. Gold nanoparticles of 15 and 50 nm have been found out in the form of clusters in the cytoplasm of macrophages and lymphocytes mainly in the mantle zone of lymphoid follicles and brain strands. Conclusion: It has been established that the gold nanoparticles of 15 and 50 nm has been detected in the form of clusters in the cytoplasm of macrophages and lymphocytes.

AttachmentSize
2013-01_017-020.pdf266.58 KB