Saratov JOURNAL of Medical and Scientific Research

Saratov state university

Comparative evaluation of autonomic regulation of circulation in patients undergone coronary artery bypass grafting or correction of acquired valvular heart disease

Year: 2018, volume 14 Issue: №1 Pages: 45-49
Heading: Internal Diseases Article type: Short message
Authors: Kiselev A.R., Vulf K.A., Shvartz V.A., Karavaev A.S., Borovkova E.l., Bockeria O.L.
Organization: Bakulev Center of Cardiovascular Surgery, Saratov State Medical University n.a. V.I. Razumovsky Research Institute of Cardiology, Saratov state university
Summary:

The aim of the research was to study the peculiarities of vegetative regulation of blood circulation in cardiac surgery patients who underwent coronary artery bypass grafting (CABG) or correction of acquired valvular heart disease (CAVHD). Material and Methods. In this study we included 42 patients (12 women; 63 (57; 67) years), who underwent CABG, and 36 patients (16 women; 58 (47; 65) years) who underwent CAVHD. The synchronous 15 minutes records of electrocardiogram and photoplethysmogram (PPG) were performed in all patients before and after surgery. Time domain and frequency domain measures of heart rate variability (HRV) and index of synchronization between low-frequency (LF) oscillations in HRV and PPG (index S) were analyzed. Results. Most studied autonomic indices did not have statistically significant differences between patients with CABG and CAVHD in the study stages, except for heart rate, which was higher in patients before CAVHD (p=0,013). Conclusion. The values of HRV and index S do not depend on the difference in the clinical status and the features of performed cardiac surgical interventions between patients with CABG and CAVHD.

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Peculiarities of microcirculatory reactions after subcutaneous implantation of polycaprolactone matrices mineralized by vaterite

Year: 2018, volume 14 Issue: №1 Pages: 35-41
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Norkin I.A., Ivanov A.N., Kurtukova M.O., Savelyeva M.S., Martyukova A.V., Gorin D.A., Parakhonsky B.V.
Organization: Saratov State Medical University, Saratov state university, Skolkovo Institute of Science and Technology
Summary:

The aim is to assess skin microcirculation changes arising during subcutaneous implantation of polycaprolactone scaffolds mineralized by vaterite. Material and Methods. The experiment was carried out on 30 albino rats divided into two groups: a negative control group and an experimental one. We implanted polycaprolactone scaffold with exhausted foreign protein subcutaneously into rats of the negative control group. We implanted polycaprolactone matrix mineralized by vaterite subcutaneously into the animals of the experimental group. Research methods include laser Doppler flowmetry and morphological examination of the tissues of the matrix implantation area. Results. Changes of skin microcirculation over the matrix allocation area correspond to the morphological pattern of tissue reactions. Biocompat-ibility disorders take the form of inflammation in the scaffold implantation area that is accompanied by stable perfusion rise associated with local bloodstream modulation changes. We did not observe inflammation signs in the surrounding scaffold tissues during implantation of polycaprolactone matrices mineralized by vaterite. Besides local microcircula-tory reactions possess a transient character disappearing completely by the 21st day after the implantation. Conclusion. Complex of the given functional and morphological studies allow us to ascertain high-grade biocompatibility of polycaprolactone matrices mineralized by vaterite thus giving rise to prospect of their use for tissue regeneration stimulation.

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Vitiligo: modern methods of therapy

Year: 2017, volume 13 Issue: №3 Pages: 668-673
Heading: Dermatovenerology Article type: Review
Authors: Talnikova Е.Е., Svenskaya Yu.l., Dobdina A.Ju., Fatakhova Kh.V., Utz S.R.
Organization: Saratov State Medical University, Saratov state university
Summary:

This brief review focuses on the main issues of vitiligo treatment. The article provides insight into modern methods of dermatosis therapy discussing their potential, efficiency, safety, as well as represents a variety of standard treatment regimens.

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Dynamics of some biophysical parameters of skin under the influence of chitosan-containing hydrogel.

Year: 2017, volume 13 Issue: №3 Pages: 616-620
Heading: Dermatovenerology Article type: Original article
Authors: Galkina Е.М., Shipovskaya А.В., Gegel' N.O., Artemenko S.A., Utz S.R.
Organization: Akripol LLC, Saratov State Medical University, Saratov state university
Summary:

Objective: to conduct a clinical study of the effectiveness of the gel based on chitosan in skin care healthy people with different manifestations of signs of aging and patients with pigmented lesions of the skin of those aged 34 to 63 years. Material and Methods. The study was performed in the group of patients consisting of 10 people ranging in age from 34 to 63 years. They have been estimated by the following biophysical parameters: the moisture level and elasticity of the skin, the melanin content, the level of erythema, as well as the width and depth of wrinkles. Results. The application of hydrogel with chitosan marked hydration of the skin (in particular immediate increase in skin moisture immediately afterthe first application), reducing the depth and width of wrinkles, restoring elasticity and improving muscle tone. Conclusion. Course application of the hydrogel during the month allows you to obtain a lasting effect, OMO application.

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Nail psoriasis: the first experience of photodynamic curcumin therapy.

Year: 2017, volume 13 Issue: №3 Pages: 600-604
Heading: Dermatovenerology Article type: Original article
Authors: Utz S.R., Tal'nikova Е.Е., Sultanakhmedov E.S., Myasnikova T.D., Safonov V.A., Popovicheva O.A.
Organization: Saratov state university, Saratov State Medical University
Summary:

Aim: to determine the effectiveness of the photosensitizer "Curcumin" in various concentrations (0.15%, 1%, 1.5%) in combination with visible spectrum radiation in the treatment of nail psoriasis. Material and Methods. 6 patients (4 women and 2 men) of different age groups (from 20 to 50 years) with the established diagnosis of "Psoriasis" were observed. The study was carried out using the "Curcumin" photosensitizer solution, the broadband spectrometer "Ocean Optics USB4F01891", the LS-55 spectrometer, the PhotoDyn-750 and the THORLABS PM100D. To assess the dynamics of the pathological process, the N-NAIL Index was used. Results. The most pronounced and clinically significant changes were detected when using a 1.5% solution of the "Curcumin" photosensitizer. Conclusion. The photosensitive agent "Curcumin" is effective in photodynamic therapy of psoriasis of the nails.

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Assessment of possibilities of transmission polarization mapping for the characterization of sclera macrostructure

Year: 2017, volume 13 Issue: №2 Pages: 435-441
Heading: Ophtalmology Article type: Original article
Authors: Shvachkina M.E., Pravdin А.В., Tikhonov D.A., Kamenskikh T.G., Yakovlev D.D., Yakovlev D.A.
Organization: Saratov state university, Saratov State Medical University
Summary:

The purpose of the study is to assess possibilities of applying the method of transmission polarization mapping for characterization of sclera structure. Material and methods. In the work, samples of rat, rabbit, porcine, and human sclera were examined. Samples of the human sclera were taken (enucleation of the eyeball) from patients with diagnoses: terminal painful glaucoma, subatrophy of the eyeball. From the enucleated eyes, sclera samples were taken from the posterior pole of the eye and from the equator. The sample transparency required for carrying out transmission polarization mapping in thick samples was achieved using immersion optical clearing, 85% and 50% aqueous glycerol solutions and 40% aqueous glucose solution being used as immersion agents. The polarization mapping system used in this work was assembled on the basis of a polarization microscope equipped with a measuring video camera. Re-sults. It is shown that the method of transmission polarization mapping can be used to measure the parameters of the orientational order of scleral collagen fibers, mapping of human sclera samples requiring conduction of the polarization measurements under optical clearing conditions. Since the disorganization of collagen structures of eye connective tissue can be considered as the initial stage of glaucoma process, the technique of transmission polarization mapping can be used as a basis for in vitro studies of the pathogenesis of primary open-angle glaucoma. Conclusion. The potential and limitations of polarization mapping in studying sclera structure in vitro have been established.

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Development of ophthalmic training simulator for cataract surgery

Year: 2017, volume 13 Issue: №2 Pages: 417-420
Heading: Ophtalmology Article type: Original article
Authors: Kumova S.V., Lunev I.A., Gasparyan E.A., Virsta A.M., Batishcheva Yu.S., Kamenskikh T.G., Dolinina O.N.
Organization: Saratov State Medical University, Saratov state university, Yuri Gagarin State Technical University of Saratov
Summary:

Purpose: development of interactive simulator for the development of phacoemulsification. Material and Methods. Material and methods: the Software developed by the authors ophthalmic virtual simulator has several modules that can also be complemented by depending on put before the students the task. Testing the virtual simulator "Phacoemulsification" held at the Department of ophthalmology with the participation of 20 interns and 18 residents. Results. The development of residents and interns of ophthalmological profile of the theoretical foundations of cataract surgery using developed by the authors of the publication virtual interactive simulator demonstrated the effectiveness of this technique in comparison with passive viewing of video recording operations and the familiarity with the course of phacoemulsification in the operating room. Conclusion. The application of the developed simulator with the purpose of optimization of educational process of residents and interns of ophthalmic profile is a very effective tool in the world today.

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ОСТ investigation of influence of cement kiln dust and keratoprotectoral medication on the cornea structure.

Year: 2017, volume 13 Issue: №2 Pages: 400-406
Heading: Ophtalmology Article type: Original article
Authors: Kalmykov R.V., Popova D.V., Kamenskikh T.G., Genina E.A., Tuchin V.V., Bashkatov A.N.
Organization: National Research Tomsk State University, Saratov State Medical University, Saratov state university
Summary:

The research goal: in vitro study of cornea permeability at the action of cement kiln dust and keratoprotectoral medication. Materials and Methods. Experimental studies were carried out with twenty eyes often rabbits. The measurements were performed using ОСТ system OCP930SR 022 (Thorlabs, USA). Results. ОСТ tomography was used to monitor the attenuation coefficient of the cornea in vitro during the permeability of cement kiln dust and keratoprotector Systein Ultra. The permeability coefficients of the cornea for water, cement dust and keratoprotector were measured. A computer model was developed that allowed one to analyze the diffusion of these substances in the eye cornea. Conclusion. It is shown: 1) cement dust falling on the eye cornea caused pronounced dehydration of the tissue (thickness decreasing) and led to the increase in the coefficient of light attenuation, which in the conditions of cement production could affect the deterioration of the eyesight of workers; 2) the application of the keratoprotector on the eye cornea when exposed by cement dust, slowed significantly the dehydration process and did not cause the increase in the light attenuation coefficient, which characterized the stabilization of visual functions in cement production workers. In this case, the keratoprotector itself did not cause dehydration and led to the decrease in the light attenuation coefficient, which could allow it to be used for a long time in the order to protect the organ of vision from the negative effects of cement dust.

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Clinical and immunological parallels in the diagnosis of primary open-angle glaucoma.

Year: 2017, volume 13 Issue: №2 Pages: 378-383
Heading: Ophtalmology Article type: Original article
Authors: Kamenskikh I.D., Kamenskikh T.G., Kolbenev I.O., Zakharova N.B., Kozletsov A.P., Tuchin V.V.
Organization: Saratov State Medical University, Saratov state university, Yuri Gagarin State Technical University of Saratov
Summary:

The purpose of study is an assessment of the informative value of various diagnostic parameters in monitoring and prognosis of the state of the visual organ in patients with primary open-angle glaucoma. Material and methods. 216 patients (216 eyes) were examined. 126 patients (126 eyes) with the diagnosis of primary open-angle glaucoma I, II or III stage were included in the main group (I), and 90 patients (90 eyes) without ophthalmologic pathology were included in the control group (II). All patients underwent complex examination. Laser confocal scanning tomography for the analysis of the optic disc morphology was performed using a Heidelberg Retina Tomograph II from Heidelberg Engineering (Germany). Evaluation of the field of view was carried out using the computer static perimeter Oculus twinfield-2 (Germany). Electrophysiological studies (apparatus Roland Consult, Germany) included: the study of visual cortical potentials on the outbreak, the pattern-electroretinogram, photopic negative response. Neurotrophic brain factor, ciliary neurotrophic growth factor and protein NF-200, S-100 protein, monocyte chemoattractant, vascular endothelial growth factor were determined in the serum. Results. An increase in the concentration of the S-100 protein with progression of POAG testifies to the existence of parallels between apoptosis of retinal ganglion cells and systemic neurodegenera-tion affecting the central nervous system as a whole. The importance of electrophysiological research methods, which give information about the third neuron of the visual pathway, has been confirmed. Pattern-electroretinogram, photopic negative response most reflect abnormalities in the work of ganglion cells of the retina, including before the appearance of structural changes in the optic disc and the retina. Conclusion. The interrelation of dynamics of functional, morphological and immunological criteria of progression of POAG is revealed, which allows to predict the further course of the pathological process. Since the study involved patients with IOP target values, it is important not only to maintain IOP on target values, but also to carry out neuroprotection.

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Efficiency of nistagmus treatment method using videooculography in a long-term observation

Year: 2017, volume 13 Issue: №2 Pages: 364-369
Heading: Ophtalmology Article type: Original article
Authors: Doroshenko А.А., Radevich S.B., Postelga А.Е., Usanova Т.В., Kamenskikh T.G., Usanov D.A.
Organization: Saratov State Medical University, Saratov state university
Summary:

The purpose of the study was to investigate the efficiency of nistagmus treatment method using videooculography in a long-term observation. Material and methods. 288 patients were examined. The nystagmogram had been record-ering at the time of the patient's admission, every day after the treatment and after the entire course of treatment. The video image had been recordering for 1 minute at the sight of the patient directly. Based on the diagnostic results, there had been determining the following parameters: the main nystagmus frequency, the amplitude of the nystagmus (maximum deviation from the central position). The treatment procedure had been carrying out for 7-10 minutes in average, depending on the degree of decrease in the amplitude of the nystagmus. It is necessary to correct the frequency of light stimulus when the main nystagmus frequency changes. Results. Suppression of nystagmus in patients after a single treatment procedure persisted for different times (from several seconds to several minutes), after which the gradual recovery of the nystagmus occurred before the values of the amplitudes less than before the start of the treatment procedure. The result of repeated procedures was a further decrease in nystagmus amplitudes and an increase in the time of preservation of the suppression effect. One course included 7-10 treatment procedures. On average, for 2 courses of treatment per year in 60% of patients the amplitude of nystagmus decreased by 40-85%, in 30% of patients the amplitude of nystagmus did not change more than 8-12%, in 10% of patients there were no significant changes. Conclusion. The developed method using videooculography is effective in the diagnosis and treatment of nystagmus, as it allows to reveal the frequency of nystagmus of each patient with further treatment by light stimulus of the patient at this frequency and the possibility of its correction.

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