Saratov JOURNAL of Medical and Scientific Research

Shevchenko O.V.

Saratov State Medical University

Association of polymorphism of CYP2D6 and CYP2C9 genes encoding P-450 proteins of cytochrome with arterial hypertension

Year: 2012, volume 8 Issue: №4 Pages: 933-937
Heading: Internal Diseases Article type: Original article
Authors: Borodulin V.B., Shevchenko O.V., Bychkov E.N., Kiselev A.R., Reshetko O.V., Posnenkova O.M., Saratsev A.V., Losev O.E.
Organization: Saratov State Medical University
Summary:

Gene polymorphisms of cytochrome P-450 CYP2 encoding proteins of cytochrome P-450 are essential forantihy-pertensive drugs metabolism. Purpose: We study the associations of functionally defective allele variants of CYP2D6 gene and CYP2C9 gene with the degree of arterial hypertension (AH). Materials and methods: Samples of DNA of leukocytes of blood of 150 patients with AH without the associated clinical conditions (56% of women) at the age of 20-59
years have been investigated. For the study of polymorphism of genes the pharmacogenetic biochip developed in the Institute of Molecular Biology n.a. V. A. Engelgardt has been used. Comparison of frequencies of occurrence of signs has been carried out on the basis of chi-square criterion. Results: It has been revealed that homozygotes by mutant A1075C, C430T alleles of CYP2C9gene and G1934A of CYP2D6 gene have been significantly more common among patients with hypertension III (p=0.01). Conclusion: The research works on genes of system of P-450 cytochrome have important clinical value for rationalization of pharmacotherapy of hypertension. The increased frequency of occurrence of mutant allele of CYP2D6 and CYP2C9 genes in patients with hypertension III requires special attention to the problem of efficiency and safety of application of hypotensive drugs for the patients.

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The role of genetic mutations in development of metabolic disturbances in patients with arterial hypertension

Year: 2012, volume 8 Issue: №3 Pages: 751-756
Heading: Internal Diseases Article type: Original article
Authors: Borodulin V.B., Shevchenko O.V., Bychkov E.N., Reshetko O.V., Kiselev A.R., Posnenkova O.M., Zhelezinskaja N.V., Sarattsev A.V., Losev O.E.
Organization: Saratov State Medical University, Saratov Institute of Cardiology, Russia, First Moscow State Medical University n.a. I.M. Sechenov, Clinical Hospital of Ministry of Internal Affairs of the Russian Federation
Summary:

The article stresses on the study of molecular markers of essential arterial hypertension providing insight into the pathological mechanisms underlying the disease and creation of «genetic passport» for every patient in the future. Objective: To study genetic markers of 5,10-methylenetetrahydrofolate reductase (MTHFR), involved in the metabolism of homocysteine, and N-acetyltransferase 2 (NAT2), ensuring the process of transformation of acetyl-CoAto acetoacetyl-coenzyme A. Methods: The study involved 160 patients with essential hypertension l-lll stages Caucasian (57.5% female.) aged 20-59 years. MTHFR gene polymorphisms and NAT2 in DNA of peripheral blood leukocytes were studied using a set of Wizard Genomic DNA Purification Kit (Promega, USA). Results: It was shown that «slow acetylators» (homozygous for a mutant version of the gene NAT2) were more frequently (p <0.05) determined among patients with hypertension stage 1. It was also revealed the dependence on the presence of hypertension stage mutant allele T (hetero-and homozygous variants) in 677 nucleotide gene MTHFR (r = 0,40, p <0.001). Conclusions: The regularities of the distribution of polymorphic variants of MTHFR and NAT2 gene were found out in hypertensive patients according to the stage of the disease.

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Genetic pathogenesis of essential arterial hypertension (the review)

Year: 2011, volume 7 Issue: №1 Pages: 083-087
Heading: Internal Diseases Article type: Review
Authors: Shevchenko O.V., Svistunov А.А., Borodulin V.B., Ruta A.V., Bychkov E.N.
Organization: Saratov State Medical University, First Moscow State Medical University n.a. I.M. Sechenov
Summary:

It is proved that genetic factors may be considered as fundamental in the development of essential arterial hypertension (AH). Important role in this process belongs to the genes whose products are involved in regulating blood pressure - adrenergic, renin-angiotensin-aldosterone, and homocyclic bradykinin systems. These systems are closely connected by serial and parallel chemical reactions that enable to use genetic testing to determine the state of the whole system. Analysis of polymorphic markers of different groups of genes encoding components of the renin-angiotensin-aldosterone system allows to define the role of each of the pathogenic factor of hypertension in the development of the disease

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Peculiarities of bone metabolism in patients with multiple myeloma

Year: 2010, volume 6 Issue: №1 Pages: 048-052
Heading: Physiology and Pathophysiology Article type: Original article
Authors: A.A. Svistunov, A.V. Ruta, O.V. Shevchenko
Organization: Saratov State Medical University
Summary:

Multiple myeloma is one of the most widespread hemoblastosis. In the structure of oncohematology it takes up about 10%. It follows with destruction of bones and results in lethal outcome. Ostealgia’s syndrome is leading in more than 70% of patients at the beginning of this medical condition. Damage of the skeleton is found at 80% of patients by means of X-ray. Patients suffer from decrease of osseous tissue mineral density and increase in level markers of osseous resorption and elaboration (bones acid and alcaline phosphatases, desoxypiridoline). These indices can be used as predictors of efficiency and safety of treatment of osteopenia in patients with multiple myeloma

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THE DYNAMICS OF 0,1 HZ-COMPONENT OF HEART RATE VARIABILITY AT PATIENTS WITH ARTERIAL HYPERTENSION AT TREATMENT BY FOSINOPRIL AND ATENOLOL

Year: 2008, volume 4 Issue: №1 Pages: 84-87
Heading: Internal Diseases Article type: Original article
Authors: O.V. Shevchenko, A.R. Kiselev, V.I. Gridnev, O.M. Posnenkova, A.N. Strunina, A.A. Svistunov
Organization: Saratov Institute of Cardiology, Russia, Saratov State Medical University
Summary:

Aim: to study dynamics of the 0,1Hz-componentofthe heart rate variability(HRV) spectrum in patients with arterial hypertension taking fosinopril and atenolol. 49 patients with hypertension were enrolled (age - 46+/-7 years). Fosinopril and atenolol were administered consequently. The power of 0,1 Hz-component of the HRV spectrum was assessed during a passive orthostatic test. Results: In patients taking fosinopril and atenolol comparable decrease of blood pressure was observed. Atenolol caused reliable decrease of a heart rate during orthostatic test. The power dynamics of 0,1Hz-componentwas comparable in patients taking fosinopril and atenolol. Conclusion: fosinopril and atenolol influenced similar on 0,1-Hz mechanisms of vegetative heart control system.

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