Saratov JOURNAL of Medical and Scientific Research

Military Medical Academy n.a. S. M. Kirov

On preparation of am-niotic membrane as a scafold for cultivated cells to create corneal bioengineering constructs

Year: 2019, volume 15 Issue: №2 Pages: 409-413
Heading: Ophtalmology Article type: Original article
Authors: Aleksandrova OI, Gavrilyuk IO, Mashel TV, Chernysh VF, Churashov SV, Kulikov AN, Blinova MI.
Organization: Institute of Cytology, Russian Academy of Sciences, Military Medical Academy n.a. S. M. Kirov, St. Petersburg Polytechnic University
Summary:

Aim: to determine the preparation of amniotic membrane (AM) necessary for its use as a scafold for cultured cells to create bioengineered constructions (BEC). Material and Methods. Native AM was placed in a special clamping device and subjected to additional mechanical, thermal and enzymatic treatment: removal of mucus residues from its surface and cryoconservation of AM scafolds was performed at –80°C, — 20°C with subsequent decellularization with a 0.25 % Tripsin-EDTA mixture. The lifetime assessment of the morphology of cells cultivated on AM scafolds was performed using a Nikon Eclipse TS100 inverted microscope equipped with camera. The viability and metabolic activity of AM cells was determined by means of an MTT test using a UNIFLAN AIFR-01 tablet spectrophotometer (Picon, Russia) at a wavelength of 570 nm and a reference wavelength of 620 nm. Results. It has been established that the presence of mucous residueson the surface of native AM, which are not removed during standard mechanical processing, negatively afect the survival of the cell test system. The efects of cryoconservation of AM scafolds revealed the positive efects of this process together with enzymatic decellularization for improving the viability of cells cultured on scafolds. Conclusion. Standard mechanical processing of native AM does not guarantee complete cleaning of its surface from mucus residues that interfere with adhesion and even distribution of cultured cells. It is necessary to reliably control the removal of AM surface mucus before immobilization and further manipulations. Cryopreservation and subsequent decellularization of AM scafolds contributes to the increased viability of the cell test system. AM scafolds, purifed from amniotic mucus, cryopreserved at –80 ° C in a mixture of DMEM-F12 and DMSO (1:1) and enzymatic decellularization after thawing, turned out to be the best of the studied substrates for cell cultivation.

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Techniques of augmentation and full-endoscopic decompression in the treatment of patients with vertebral fractures of osteoporotic origin

Year: 2018, volume 14 Issue: №3 Pages: 412-416
Heading: neurosurgery Article type: Case report
Authors: Kravtsov M.N., Mirzametov S.D., Svistov D.V.
Organization: Military Medical Academy n.a. S. M. Kirov
Summary:

The article highlights debatable questions concerning the effectiveness of surgical treatment of osteoporotic vertebral fractures. The reasons of unsuccessful outcomes of vertebroplasty are analyzed. A combined method of surgical treatment is proposed, including vertebroplasty and full-endoscopic foraminotomy with a combination of local and ra-dicular pain syndrome caused by stenosis of the intervertebral foramen. A clinical example is given.

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Evaluation of latent period of temperature sensitivity in traditional and unilateral spinal anesthesia

Year: 2015, volume 11 Issue: №3 Pages: 263-267
Heading: Anaesthesiology and Reanimatology Article type: Original article
Authors: Lakhin R.E., Panov V.A., Schegolev A.V., Kuligin A.V.
Organization: Military Medical Academy n.a. S. M. Kirov, Saratov State Medical University
Summary:

Objective: evaluation of the differences in the level of temperature, sensory and motor blockade during the various techniques of spinal anesthesia. Materials and Methods. Prospectively the randomized study included 70 patients. In the group with conventional spinal anesthesia (n=35) 15mg of bupivacaine injected quickly. In the group with unilateral spinal anesthesia (n=35) 7.5mg of bupivacaine administered slowly, at a rate of 1 ml per minute. We studied the level of temperature and time, sensory and motor blockade. The data are statistically processed. Results. Reduced thermal sensitivity recorded in an average of 48 seconds as a unilateral spinal anesthesia group and the control group. Subarachnoid administration of 7.5 mg of hyperbaric bupivacaine resulted in the development of a complete motor blockade underlying lower extremity in only 16 patients (45.7%). Conclusion. Using lower dosages of bupivacaine for unilateral spinal anesthesia lowers the threshold concentration of the local anesthetic in the subarachnoid space, resulting in slower motor neuron blockade and it is not total in more than 50% of cases.

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Low dose spinal anesthesia for knee arthroscopy

Year: 2015, volume 11 Issue: №2 Pages: 126-128
Heading: Anaesthesiology and Reanimatology Article type: Original article
Authors: Lakhin R.E., Schegolev A.V., Panov V.A., Kuligin A.V.
Organization: Military Medical Academy n.a. S. M. Kirov, Saratov State Medical University
Summary:

Objective: to evaluate the nature of unilateral spinal anesthesia using various modes of administration of low doses of hyperbaric bupivacaine. Materials and Methods. Prospectively, the randomized study included 56 patients undergoing knee arthroscopy. In the control group bupivacaine of 5mg was administered simultaneously, in the main group — fractionally by 2.5 mg. The development of thermal and pain blocks from different sides was investigated. The data were statistically processed. Results. In the control group, the positioning of the patient usually began after the entire dose of anesthetic had been administered. In the case of temperature paresthesia in the area of the sacral segments of the full anesthesia throughout underlying limb was not always achieved. In 6 cases of block was not sufficient. In the main group patient positioning was performed after the administration of 2.5 mg of anesthetic and evaluate temperature paresthesia and in 2 cases the total dose was increased to 7.5 mg. The successful development of sensory block at fractional administration was significantly higher than in the single-step introduction. Conclusion. Temperature paresthesia occurs within the first minute and is an early predictor of developing spinal anesthesia. The area of arising paresthesia shows preferential distribution of the anesthetic. In the application of low dose local anesthetic the desired upper level of anesthesia via the patient positioning and dose adjustment may be achieved.

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Assessment of efficiency of the strengthening mode of ultrasonic visualization of needles for peripheral regional anaesthesia

Year: 2014, volume 10 Issue: №4 Pages: 612-616
Heading: Anaesthesiology and Reanimatology Article type: Original article
Authors: Kuligin A.V., Panov V.A., Lakhin R.E., Shchegolev A.V., Yatsenko D.V., Kotikova M.N.
Organization: Military Medical Academy n.a. S. M. Kirov, Saratov State Medical University
Summary:

Research goal: optimization of peripheral regional anaesthesia under ultrasound. Material and Methods. Study has been performed by SonoSite Edge (SonoSite, Bothell, США) on a lineal transducer machine for fixed terms 38-mm (13-6 MHz) of overall gain and depth. The study included 2 sonographic needles and 2 non-sonographic needles. Ultrasound images were obtained and analyzed by an experienced anaesthesiologist. Results. In this study improved ultrasound imaging needle for peripheral regional anaesthesia using technology SonoMBe compared with a conventional scanning mode has been demonstrated. Conclusions. SonoMBe technology increases the echogenicity of needles for peripheral regional anaesthesia that allows the use of this technology gain in the performance of nerve block and plexus block anesthesia

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Chronic pain syndrome at tunnel neuropathies of peripheral nerves. integrative approaches to therapy

Year: 2012, volume 8 Issue: №2 Pages: 371-374
Heading: Neurology Article type: Original article
Authors: Andreyeva G. O., Yemelyanov A. Y.
Organization: Military Medical Academy n.a. S. M. Kirov
Summary:

Painful chronic tunnel syndrome is almost always connected with asthenia and a various degree of anxiety and depressive syndrome. The goal of the research is to investigate prevalence and expressiveness of depressive and astheniс disorders in patients with chronic tunnel pain syndrome and to fnd accommodation of choice. 54 patients with chronic painful tunnel syndrome (35 (64,8 %) male, 19 (35,2 %) female, mean age 39,7+10,9 years) have been examined and treated. Different acupuncture methods in complex treatment were accompanied by a positive dynamics of both subjective and objective clinical neurological and psychological symptoms. Anxiety have been revealed in 79,6 % of patients, depressive syndrome — in 57,3 % of patients, asthenia — in 85,2 % of patients. Acupuncture is tolerable, compare and have not got backside effects. Inclusion of acupuncture in complex treatment chronic tunnel pain syndrome allows to reduce a pain syndrome and asthenia, to normalize psychological condition of the patient.

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