Saratov JOURNAL of Medical and Scientific Research

Ponukalina E.V.

Saratov State Medical University

. Modern concepts on carcinogenesis: the value of insufficiency of malignant cell elimination mechanisms in the tumor progression in case of neoplasia at different localizations

Year: 2016, volume 12 Issue: №1 Pages: 32-35
Heading: Physiology and Pathophysiology Article type: Review
Authors: Chesnokova N.P., Morrison V.V., Barsukov V.Yu., Ponukalina E.M., Agabekov A.l.
Organization: Saratov State Medical University
Summary:

The article presents the review of contemporary concepts of tumor induction mechanisms applicable for neoplastic transformation, promotion and proliferation. The paper also contains an in-depth analysis of results of authors' own observations and study of immunological protection mechanisms of a significant number of patients diagnosed with oncopathologies of mammary and thyroid glands as well as adenocarcinoma of ascending colon. We have established common development patterns of T-cell and B-cell immunodeficiencies that are not influenced by location and proliferation of neoplastic tumors; the progress of such immunodeficincies, however, depends on regional metastasis development. Thus, we can conclude that insufficiency of immunological defense mechanisms leading to disruption of maligned cell elimination process is the primary pathogenic factor for metastatic tumor development.

AttachmentSize
2016_01_032-035.pdf260.74 KB

Molecular and cellular mechanisms of destabilization of membranes and prenatal discharge of amniotic fluid

Year: 2013, volume 9 Issue: №4 Pages: 644-648
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Mikhaylov А.V., Dyatlova L.I., Chesnokova N.P., Ponukalina Е.V., Glukhova T.N.
Organization: Perinatal Centre, Saratov , Saratov State Medical University
Summary:

Purpose: to establish the role of activation of lipid peroxidation and antioxidant deficiency of blood and amniotic fluid in the destabilization of membranes with early prenatal discharge of amniotic fluid. Material and methods. The analysis of lipid peroxidation products content in blood and amniotic fluid — diene conjugates (DC), malondialdehyde (MDA) and the quantitative determination of total peroxides (OxyStat), the estimation of the total antioxidant status (TAS), the activity of SOD and the content of ceruloplasmin in 20 pregnant with premature rupture of membranes in the gestation period of 22-34 weeks, were hospitalized at the Department of Pathology of Pregnancy of Saratov Perinatal Center in 2012. DC and MDA determination was carried out by conventional spectrophotometric methods of research, quantitative determination of peroxides (OxyStat) was carried out by enzyme immunoassay analyzer «Alfa Prime» («Meredith Diagnostics», 2008 using chemicals made by the company «Bender Medsystems»). The study of antioxidant status of serum and amniotic fluid was performed by enzyme immunoassay analyzer «Alfa Prime» («Meredith Diagnostics», England, 2008). Reagents («Bender Medsystems», Austria) were used to determine the total antioxidant status (TAS) and the SOD activity. To assess the content of ceruloplasmin reagent «Sentinel» (Italy) was used. Results. Indicators of lipid peroxidation in blood and amniotic fluid and anti-radical protection of biological membranes of cells and body fluids in the "mother-placenta-fetus" increased. Conclusion. Systemic activation of lipid peroxidation has been proved to be a
pathogenetic factor in the failure of membranes in prenatal discharge of amniotic fluid. The increase in the blood levels of lipid peroxidation products on the background of the activation of antioxidant system is considered as a prognostic sign of abortion.

AttachmentSize
2013-04_644-648.pdf322.34 KB

Changes in the cytokine profile of patients blood with premature rupture of amniotic membranes and their pathogenic significance

Year: 2013, volume 9 Issue: №2 Pages: 224-229
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Mikhaylov А.V., Dyatlova L.I., Chesnokova N.P., Ponukalina E.V., Glukhova T.N.
Organization: Saratov State Medical University, Perinatal Centre, Saratov
Summary:

Purpose: Pathogenetic study of new diagnostic and prognostic criteria for evaluation of pregnancy in terms of the cytokine profile of blood. Materials and methods: The analysis of anti-inflammatory and pro-inflammatory cytokines in the blood of 50 pregnant women with preterm rupture of membranes in gestation of 22-34 weeks, who were treated at the Department of Pathology in pregnancy of Saratov Perinatal Center in 2012, was performed. The method of enzyme linked immunosorbent assay using test systems «Vector— Best» (Novosibirsk, Russia). Results:.Clinical and laboratory examination revealed an increase levels of IL-1(3, IL-2, IL-6, IL-8, TNF-a, while reducing the content of IL-4, IL-10 in blood. Conclusion: Manifest signs of possibility of prenatal rupture of membranes and premature labour in 22-34 weeks gestation are combined with increasing of IL-1(3, IL-6, IL-8, TNF-a, IL-2 and with decreasing of IL-10, IL-4 level in the blood, which indicates the need for monitoring the content of these cytokines in the blood for the diagnosis and prognosis of pregnancy.

AttachmentSize
2013-02_224-229.pdf283.73 KB

Haemopoiesis and its regulation at various stages of haemopoietic cell differentiation of bone marrow

Year: 2012, volume 8 Issue: №3 Pages: 711-719
Heading: Physiology and Pathophysiology Article type: Review
Authors: Chesnokova N.P., Morrison V.V., Ponukalina Е.V., Zhevak Т.N., Afanasieva G.A., Polutova N.V., Newazhay Т.A.
Organization: Saratov State Medical University
Summary:

Analysis of literature review has presented authors' opinion about regularities of consistent change of haemopoietic cell differentiation steps in bone marrow. Particular attention has been paid to the role of numerous cytokines (stem cell factor, fit-3ligand, interleukine-3, interleukine-4, interleukine-6, interleukine-7, tumor necrosis factor, colony stimulating factors, erythropoietin, thrombopoietin) in mechanisms of myelo-, erythro- and thrombocytopoiesis in the normal conditions. Results of investigation have showed the important significance of blood cytokine profile disturbances in pathogenesis of tumor progression in chronic lymphocytic leukaemia.

AttachmentSize
201203-711-719.pdf577.73 KB

ABOUT THE ROLE OF ENDOTHELIAL DYSFUNCTION IN GESTOSIS DEVELOPMENT

Year: 2007, volume 3 Issue: №3 Pages: 60-62
Heading: Obstetrics and Gynecology Article type: Original article
Authors: G.I. Khripunova, E.V. Ponukalina, G.S. Salakhieva
Organization: Saratov State Medical University
Summary:

The complex clinical and laboratory researches conducted in groups of the pregnant women with mild case of gestosis, middle serious preeclampsia and with heavys everity of gestosis. Anticoagulation and fibrinolytic activity of a wall of vessels on a background of creation of a short-lived ischemia were examined. Disturbance of a coagulative potential of a blood are actuated development endothelial dysfunction in groups of the pregnant women with middle serious preeclampsia and with heavy severity of gestosis.

AttachmentSize
200703_060.pdf106.92 KB

THE SARATOV SCIENTIFIC SCHOOL OF HEMOSTASIOLOGY

Year: 2007, volume 3 Issue: №1 Pages: 8-15
Heading: History of Medicine. Anniversary Dates Article type: Original article
Authors: V.F. Kirichuk, E.V. Ponukalina
Organization: Saratov State Medical University
Summary:

The analysis of stages of development and perfecting of the Saratov scientific school of hemostasiology is carried cut in this article. The professor I. A. Chuevski was first decane of medical faculty and organizer of physiology chair of the Imperial University and began to form of Saratov scientific school of hemostasiology. The continuers and followers of scientific ideas, traditions, transactionses by I.A. Chuevski were professors E.S. Ivanicki-Vasilenko, S.A. Georgieva in following years. The leading directions of research activity of chair of normal physiology SGMU are examined in this work, studing the condition of a hemostasis, fibrinolysis, vascularwall, hemoreology, microcirculation, mechanisms of their regulation and correction of the detected changes in conditions of experiment animals, and also the different forms of infections and somatic pathology on the people.

DYNAMIC CHANGES OF HAEMORHEOLOGIC DATA DURING PHYSIOLOGICAL COURSE OF PREGNANCY

Year: 2008, volume 4 Issue: №1 Pages: 54-58
Heading: Physiology and Pathophysiology Article type: Original article
Authors: O.N. Hizhnyakova, E.V. Ponukalina
Organization: Saratov State Medical University
Summary:

Changes of rheological blood properties of were studied in 91 women with physiological course of pregnancy. The control group was formed of 21 nonpregnant women. During physiological course of gestation the following dynamical changes of haemorheologic data were observed: phase changes of blood viscosity and plasma viscosity, increase of
serum blood viscosity. These changes have adaptive character and promote formation of optimal conditions for fetus growth and development.

AttachmentSize
2008_01_054_058.pdf178.6 KB

DISTURBANCES OF THE VASCULAR THROMBOCYTE MECHANISM OF HEMOSTASIS IN PATHOGENESIS OF THE MICROCIRCULATORY DISORDERS IN RAPIDLY PROGRESSIVE PERIODONTITIS

Year: 2008, volume 4 Issue: №1 Pages: 51-54
Heading: Physiology and Pathophysiology Article type: Original article
Authors: E.V. Ponukalina, N.V. Bulkina, I.N. Karpenko
Organization: Saratov State Medical University
Summary:

In modern stomatology the problem ofatypicalforms ofinflammatoryperiodontaldiseases origination, namely of rapidly progressive periodontitis (RPP), has got special importance due to its widespread. The article presents one of the impotant parts of the pathogenesis- the disturbance of microcirculation processes caused by the decrease of blood clot resistencyofa vascularwall in pathogenesis ofmicrocirculatori disorders in patients with RPP. These disturbances are predetermined by endothelial dysfunction with the subsequent degradation of the clinical presentation of disease, the stomatologic status and quality of patients life.

Keywords: -
AttachmentSize
2008_01_051_054.pdf168.12 KB