Saratov JOURNAL of Medical and Scientific Research

Institute of Biochemistry and Physiology of Plants and Microorganisms of Russian Academy of Sciences

Application of automated morphometric method of estimation of expression of immunohistochemical markers in diagnostics of prostate and bladder cancer

Year: 2010, volume 6 Issue: №4 Pages: 845-849
Heading: Urology Article type: Original article
Authors: Popkov V.M., Ponukalin A.N., Maslyakova G.N., Tsmokalyuk E.N., Bucharskaya A.B., Shirokov A.A., Burov A.M.
Organization: Saratov State Medical University, Institute of Biochemistry and Physiology of Plants and Microorganisms of Russian Academy of Sciences
Summary:

The diagnostic significance of molecular markers was evaluated by immunohistochemical research of surgical and biopsy material of 106 patients with diseases of bladder and 57 patients with diseases of prostate. They received treatment at Scientific Research Institute of Fundamental and Clinical Uronephrology with monoclonal antibodies: Ki-67, PCNA, p63 (markers of proliferative activity), p53 (suppressor of tumor growth), Bcl-2, Bax (markers of apoptosis), EGFR (receptor of epidermal growth factors), PSA, PSMA, AMACR (prostate-specific antigens) and cytokeratin profile. The morphometric research was conducted on the basis of automated analysis system of digital images Ariol SL50 (Genetix)

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