Saratov JOURNAL of Medical and Scientific Research

thymus

Immuno-morphological features of the rat's thymus at the experimental administration of cytostatics

Year: 2016, volume 12 Issue: №1 Pages: 12-14
Heading: Macro- and Micromorphology Article type: Original article
Authors: Lebedinskaya Е.А., Lebedinskaya O.V., Godovalov А.Р., Prokudin V.S.
Organization: Perm State University of Medicine n.a. academician E.A. Vagner
Summary:

The aim is to identify the changes in the rat's thymus structure under immunosuppression induced injection of cyclophosphamide with the use of morpho-histochemical and morphometric methods. Material and methods. For immunosuppression of animals cyclophosphamide at 100mg/kg body weight 4-fold with a 24-hour interval was injected intraperitoneally. Thymus serial paraffin sections were stained with hematoxylin and eosin, azure ll-eosin, methyl green pyronin according to Brachet, and periodic acid-Schiff before and after treatment with amylase. Results. Significant morphological changes in the thymus at rats with injection of cyclophosphamide were established. Morpho-histochemical changes in the rat's thymus characterize the immunosuppressive state of the experimental animals' organisms. Conclusion. It was shown the necessity of complex morphological studies including macrophage activity and characteristics of the subpopulation of mononuclear leukocytes spleen, changes in the structure of hematopoietic and lymphoid organs under immunosuppression induced by cyclophosphamide.

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Effect of individual and group housing of mice on the level of radioresistance

Year: 2015, volume 11 Issue: №4 Pages: 653-656
Heading: radiation medicine Article type: Original article
Authors: Dorozhkina O.V., Bulynina Т.М., Ivanov А.А..
Organization: State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency
Summary:

Aim: to examine the effect of individual and group housing of mice on radioresistance. Material and methods. Effects of individual and group housing of mice on immunity and blood systems were studied on ICR (CD-1) and C57BI6 male mice before and after proton irradiation. Results. Group housing of intact animals resulted in a decline in the number of nucleated cells in the femur bone marrow and thymus mass. The irradiation with proton with energy of 171 MeV at a dose of 1 Gy causes a statistically significant greater reduction of the number of nucleated cells in the femur bone marrow in group-housed mice. A trend toward greater safety of the number of leukocytes in the peripheral blood and higher proliferative activity of bone marrow cells, as well as lower level of aberrant mitoses have been noted in individually-housed mice. Reduction processes in the recovery period of radiation sickness take place at a greater rate in group-housed mice. Conclusion. Group housing of male mice causes increased sensitivity of the blood and immunity systems to the effects of radiation and at the same time accelerates processes of radiation recovery.

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Kinetiks of cellular populations under influence of infra-red low-power laser irradiation

Year: 2010, volume 6 Issue: №1 Pages: 023-026
Heading: Macro- and Micromorphology Article type: Original article
Authors: I.O. Bugaeva, A.V. Egorova, O.V. Zlobina
Organization: Saratov State Medical University
Summary:

The morphokinetiks of cellular populations under the influence of infra-red low-power laser irradiation has been studied. our researchers evidenced, that the number of cortical thymocytes decreases under the influence of infra-red low-power laser irradiation. Invasion of Mast cells in to the stroma of thymus and their contact with the reticuloepithelio-cytes are the important factors for the stimulation of proliferation and differentiation of thymocytes

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