Saratov JOURNAL of Medical and Scientific Research

Tsmokalyuk E.N.

Saratov State Medical University, Department of Pathologic Anatomy, Assistant, Saratov Scientific Research Institute of Fundamental and Clinical Urology and Nephrology, Department of Pathologic Morphology, Scientific Assistant

Efficacy evaluation of various immunohistochemical markers in stage definition and prognosis of muscle invasive urinary bladder cancer

Year: 2014, volume 10 Issue: №1 Pages: 124-128
Heading: Urology Article type: Original article
Authors: Ponukalin A.N., Maslyakova G.N., Tsmokalyuk E.N.
Organization: Saratov State Medical University
Summary:

Objective: to carry out the comparative efficacy evaluation of various immunohistochemical markers in stage definition and prognosis of muscle-invasive urinary bladder cancer. Material and methods. Surgical material has included 80 patients with urinary bladder neoplasms. Clinical, histological, immunohistochemical and statistical methods of research has been used. Results. The immunohistochemical investigation has showed that only 4 markers from the 12 (Ki-67, рбЗ, p53 и EGFR) make possible to determine the stage of urinary bladder cancer and the disease prognosis. Conclusion. The proliferative activity of marker Ki-67 can be used as an auxiliary marker in the choice of volume of surgical treatment, which could predict an unfavorable outcome of disease in 81,2% of cases.

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Application of automated morphometric method of estimation of expression of immunohistochemical markers in diagnostics of prostate and bladder cancer

Year: 2010, volume 6 Issue: №4 Pages: 845-849
Heading: Urology Article type: Original article
Authors: Popkov V.M., Ponukalin A.N., Maslyakova G.N., Tsmokalyuk E.N., Bucharskaya A.B., Shirokov A.A., Burov A.M.
Organization: Saratov State Medical University, Institute of Biochemistry and Physiology of Plants and Microorganisms of Russian Academy of Sciences
Summary:

The diagnostic significance of molecular markers was evaluated by immunohistochemical research of surgical and biopsy material of 106 patients with diseases of bladder and 57 patients with diseases of prostate. They received treatment at Scientific Research Institute of Fundamental and Clinical Uronephrology with monoclonal antibodies: Ki-67, PCNA, p63 (markers of proliferative activity), p53 (suppressor of tumor growth), Bcl-2, Bax (markers of apoptosis), EGFR (receptor of epidermal growth factors), PSA, PSMA, AMACR (prostate-specific antigens) and cytokeratin profile. The morphometric research was conducted on the basis of automated analysis system of digital images Ariol SL50 (Genetix)

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Criteria of Clinical Diagnostics and Choice of Treatment Strategy in Children with Disoders of Sexual Development

Year: 2010, volume 6 Issue: №1 Pages: 178-182
Heading: Urology Article type: Original article
Authors: N.V. Bolotova, D.A. Morozov, N.U. Raygorodskaya, D.A. Zharkov, M.M. Grigorjeva, E.N. Tsmokalyuk
Organization: Saratov State Medical University
Summary:

The number of patients with disorders of sexual development (DSD) has increased in 3.54 times during the last three years. The aim is to define the structure of intersexual disorders in children from Saratov, criteria of gender assignment and surgical approach. During the period of three years (from 2006 till 2008) 58 children with DSD aged 0 - 17 were examined. Assessment of external genitals, cytogenetic investigation, hormonal tests were performed. Imaging studies included ultrasonography, cystourethroscopy and laparoscopy with histological investigation. Considering the data DSD in children from Saratov compose sex chromosome DSD - 21,4 %, Gonadal dysgenesis - 26,2%, ovotesticular DSD - 2,3%, 46,ХХ testicular DSD - 2,3 %, complete androgen insensitivity syndrome - 4,7%. The criteria of gender assignment and surgical approach have been described in the article. The research concludes that the infants with intersexual genitels should be examined in special care centers by multispecialty team. It is nessesary to perform the karyotype test and ultrasonic study in newborns to detect the genetic and gonadal gender of the child

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