Saratov JOURNAL of Medical and Scientific Research

Konjuchenko E.A.

Saratov Scientific Research Institute of Traumatology and Orthopedict, junior researcher of Laboratory and Functional Diagnostics Department

Nerve tissue growth factors as markers of evaluation of neuro-genesis processes in traumatic spinal cord disease

Year: 2014, volume 10 Issue: №3 Pages: 446-449
Heading: Traumatology and Orthopedics Article type: Original article
Authors: Uljanov V.Yu., Norkin I.A., Drozdova G.A., Konjuchenko E.A.
Organization:
Summary:

Objective: immunological differentiation effects regeneration of nerve tissue in the acute and early periods of traumatic spinal cord disease on the basis of assessment of the dynamics of content neurospecific proteins in serum affected. Material and Methods. Content of neurospecific proteins in the blood serum has been studied by enzyme immunoassay in 40 patients with spinal cord injuries. Results. Dynamics and quantitative changes in the content of chronometric neurospecific proteins in serum of patients in acute and early periods of traumatic spinal cord disease is characterized by two-phase increase in the concentration CNTF; monotonic increase in the content of NT-3 in all periods of observation; biphasic increase in the levels of NT-4 to 1-4th and 14-th day from the date of injury. Conclusion. Complex research of neurospecific levels of proteins in the serum allows one to evaluate selectively the individual components of the process of regeneration of nerve tissue in acute and early periods of traumatic spinal cord disease.

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Cytologic assessment of dynamics of reparative processes in the experimental conditionally aseptic wound under the action of complex preparation based on chitosan and metal nanoparticles

Year: 2013, volume 9 Issue: №4 Pages: 629-632
Heading: Macro- and Micromorphology Article type: Original article
Authors: Konyuchenko Е.А.. Zhandarova L.F., Babushkina I.V., Gladkova E.V., Mamonova I.A., Belova S.V., Karyakina E.V.
Organization: Saratov State Medical University
Summary:

Objective: assessment of dynamics of reparative processes with the help of cytological examination of the preparations obtained from a conditionally aseptic wound under the action of a complex preparation based on biopolymer chitosan and metal nanoparticles. Material and Methods. A conditionally aseptic wound was simulated in 40 white outbred rats. In comparison and experimental groups cytologic assessment of dynamics of reparative processes was carried out. A number of neutrophilic leukocytes and fibroblastic row perl 00 cells in the preparations is recorded on the 3rd, 5th, 7th, 10th, 14th day from the moment of formation of the wound with the use of the immersion objective. Results. It is established, that in the comparison group the cytologic pattern of impression smear was characterized by decrease in neutrophilic leukocytes and increase in fibroblastic row cells by the 14th day from the moment of formation of the wound. In the experimental group (the use of a complex preparation) more intensive reduction of neutrophilic leukocytes and a significant increase in fibroblastic row cells by the 10th day and a complete healing by the 14th day from the moment of formation of the wound has been determined. Conclusion. When treated by a complex preparation based of biopolymer chitosan and metal nanoparticles earlier occurrence of fibroblastic row cells in preparations and a significant decrease in quantity of neutrophilic leukocytes have been observed.

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Dynamics of processes of free lipid peroxidation and antioxidant protection when the development of infectious-inflammatory complications in acute and early periods of spinal cord traumatic disease

Year: 2012, volume 8 Issue: №3 Pages: 734-737
Heading: Anaesthesiology and Reanimatology Article type: Original article
Authors: Ulianov V.Yu., Bazhanov S.P., Konyuchenko EA., Shchukovsky V.V.
Organization:
Summary:

Objective: to study the dynamics of individual indicators of free radical lipid peroxidation and antioxidant defense in acute and early periods of traumatic disease of the spinal cord during the development of infectious and inflammatory complications. Material and methods. An analysis of selected indicators of free radical lipid peroxidation and antioxidant protection in the development of infectious-inflammatory complications in the acute and early periods of traumatic disease of the spinal cord. In the state of the studied groups of lipid peroxydation was evaluated on the content of malon dialdehyde, antioxidant protection — at the level of ceruloplasmin and superoxidedismutase. Results. In patients with traumatic spinal cord disease in the absence of infectious and inflammatory complications revealed monophasic increase in the concentrations of the studied parameters in the 1-4th day after injury, followed by a gradual decrease in its 30th day. With the development of traumatic disease of the spinal cord is marked as a two-phase activation of the mechanisms of lipid peroxidation and antioxidant enzyme activities of systems occurring in the 1-4th and 14th day from the date of injury with a subsequent decrease in their 30th day. Conclusion. The findings suggest that differences in the metabolic response depending on the absence or presence of infectious-inflammatory process.

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Dynamics of concentrations of immunoglobulins M, G, A classes in the blood serum in patients with bronchopulmonary complications in the acute and early periods of the cerebrospinal trauma

Year: 2010, volume 6 Issue: №4 Pages: 841-844
Heading: Traumatology and Orthopedics Article type: Original article
Authors: Konjuchenko Е.А., Ulyanov V.Ju., Puchinyan D.M., Karjakina E.V.
Organization: Saratov Scientific Research Institute of Traumatology and Orthopedics
Summary:

The article presents research data on immunoglobulin concentration of M, G, A classes in blood serum in 15 patients with bronchopulmonary complications during acute and early periods of trauma in cervical spine. Control group consisted of 15 healthy donors - volunteers. Concentration dynamics of levels of lg M, lg G, lg A in the blood serum on the 1st-4th, 7th, 14th, 21st and 30th days was studied. Hardphase method of immune analysis based on the «sandwich» principle was used. It was stated that hypogammoglobulinemia lasted during the whole acute period of spinal cord injury

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Cytological evaluation and prediction of progression of acute erosive ulcered lesions of upper parts of gastrointestinal tract in acute and early periods of cerebrospinal trauma

Year: 2010, volume 6 Issue: №3 Pages: 701-703
Heading: Traumatology and Orthopedics Article type: Original article
Authors: Norkin I.A., Ulyanov V.Yu., Konyuchenko E.A.
Organization: Saratov Scientific Research Institute of Traumatology and Orthopedics
Summary:

80 cytological preparations derived by fibrogastroduodenoscopy from 20 patients with cerebrospinal trauma at cervical part level served as the research subject. Dynamics of the progression of acute erosive ulcered lesions of mucous membrane of the upper parts of the gastrointestinal tract was studied on the basis of the cytological analyses of mucous membrane biopsy materials. In the course of our work we used endoscopic (fibrogastroduodenoscopy) and cytological research methods. Cytological analyses of mucous membrane biopsy materials were carried out on the 7th, 14th, 21st and 28th day. Biopsy material cellular composition was evaluated on the grounds of the calculation of neutrophilic leukocytes and epithelial cells with the use of an immersion objective. In so doing we registered neutrophilic leukocyte number for 100 cells and determined neutrophilic and epithelial index. Monitoring of neutrophilic leukocyte number enables to determine presence or absence of inflammatory changes in stomach mucous membrane and duodenum in different periods of cerebrospinal trauma

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CYTOMORPHOLOGICAL EVALUATION AND PROGNOSIS OF BRONCHOPULMONARY COMPLICATIONS IN ACUTE AND EARLY PERIODS OF SPINAL CORD TRAUMA

Year: 2009, volume 5 Issue: №3 Pages: 370-375
Heading: Clinical Laboratory Diagnostics Article type: Original article
Authors: E.A. Konjuchenko, V.J. Uljanov, D.M. Puchinjan, I.A. Norkin, E.V. Gladkova
Organization: Saratov Scientific Research Institute of Traumatology and Orthopedics
Summary:

There were investigated 50 cytological preparations after fibro-optic bronchoscopy of 10 patients with cervical spinal cord injuries. The dynamics of broncho-pulmonary complications of spinal cord injuries was estimated on the basis of cytological broncho-alveolar lavage fluid investigations. In the work there were used clinico-neurologic methods, radiological (computer tomography and magnetic resonance imaging), endoscopic (fibro-optic bronchoscopy) and cytomorphological investigations. Cytomorphological investigations of broncho-alveolar lavage fluid were carried out on the 3-4, 7, 14, 30th days. Cellular composition of the broncho-alveolar wash-out (endopulmonary cytogramme) was estimated by calculation of more than 100 cells in 3 fields of the immersion microscope coverage. Quantitative changes of cellular elements were taken into account with respect to normal cell amount. The results were analyzed according to the average out method.
Quantitative changes of inflammatory elements in endopulmonary cytogramme were determined by the degree of endobronchitic manifestations and were corresponding to clinico-radiological picture of development of broncho-pulmonary complications in different periods of spinal cord injury

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