Saratov JOURNAL of Medical and Scientific Research

Nechaeva O.V.

Yuri Gagarin Saratov State Technical University, Associate Professor at the Department of Ecology, Candidate of Biological Sciences

Evaluation of the wound healing capacity of the gel form of the preparation "Mellisol" on the model of experimental planar wounds

Year: 2017, volume 13 Issue: №3 Pages: 635-640
Heading: Dermatovenerology Article type: Original article
Authors: Myl'nikov A.M., Nechaeva O.V., Zayarskiy D.A., Bespalova N.V., Mudrak D.A., Navolokin N.A.
Organization: Saratov State Medical University, Yuri Gagarin State Technical University of Saratov
Summary:

Objective: to study the wound healing activity of the gel form of the "Mellisol" preparation containing nanoaggregates of flavonoids stabilized with polyazolidine ammonium modified with iodine hydrate ions on a model of experimental planar wounds of rats. Material and Methods. An investigation of the wound healing activity of the gel form of the "Mellisol" preparation was carried out in vivo in 18 white laboratory rats of the Wistar line. Simulation of experimental planar wounds was performed according to the standard procedure described in the preclinical testing manual. Results. In the course of the studies, it was established that a statistically significant (p=0.045) acceleration of healing on the 12th day of the experiment occurred in animals of the experimental group on whose wounds the gel form of the cosmetic means "Mellisol" was applied, and the complete healing of the wound defect occurred on the 21st day after operation. Conclusion. The gel form of the preparation "Mellisol" is characterized by a high level of antimicrobial and regenerative activity reliably reducing the healing time for planar wounds without the development of microbial complications.

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Оценка затрат на лечение пациентов с разными формами псориаза в условиях круглосуточного стационара

Year: 2017, volume 13 Issue: №3 Pages: 612-616
Heading: Dermatovenerology Article type: Original article
Authors: Shnaider D.A., Utz S.R., Zayarskiy D.A., Nechaeva O.V., Guskova O.P.
Organization: Saratov State Medical University, Saratov State Technical University n.a. Yu.A. Gagarin, SEH «Saratov regional dermatovenerologic dispensary»
Summary:

Aim: preliminary studies have made it possible to establish for the polymer a broad spectrum of antimicrobial activity and to attribute it to low-toxic compounds. Material and Methods, the paper presents a comparative evaluation of the treatment of mild and moderate acne with the standard preparation adapalen+clindamycin and cosmetic product Mellisol to change the indices of the dermatological acne index (DAI). Results. The use of the cosmetic product "Mel-lisol" contributed to a significant reduction in the indices of CFU of bacteria. Conclusions. Using of the cosmetic product "Mellisol" is effective in the treatment of acne and showed its high efficacy and safety in the treatment of patients with mild and moderate acne.

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Optimization of the technological cycle of formation of "core — shell" structures and the study of an- timicrobial and regenerative properties of the dispersions based on the prepared structures

Year: 2016, volume 12 Issue: №3 Pages: 462-467
Heading: Dermatovenerology Article type: Original article
Authors: Zayarskiy D.A., Gorokhovskiy A.V., Nechaeva O.V., Ul'yanov V.Yu., Tikhomirova E.l., Vakaraeva M.M., Schnaider D.A., Utz S.R., Arsenievich D.M.
Organization: Chechen State University, National Research Nuclear University "МЕРЫ", Saratov State Agrarian University named after N.I. Vavilov, Saratov State Medical University, SEH «Saratov regional dermatovenerologic dispensary»
Summary:

The aim of the work was to create optimized composite structures on the basis of nanoscale aggregates of fla-vonoids and study of their antimicrobial and regenerative properties. Material and methods. Antimicrobial activity of the resulting preparation was studied using the serial dilution method. Standard and clinical strains of Gram-positive and Gram-negative bacteria and microscopic fungi were used as experimental models. Assessment of regenerative effect of structures containing nanoaggregates flavonoids on experimental models of full thickness skin wounds was performed. Results. The high antimicrobial activity of the created structures is seen against opportunistic pathogenic microorganisms including clinical isolates resistant to multiple antibiotics. Displaying the reliable increase in rate of healing of experimental full thickness skin wounds by stimulating regenerative processes. Conclusion. Developed preparation containing "core — shell" structure stabilized by polyelectrolyte based flavonoid nanoaggregates can be considered as a potentially new type of drugs for use in cosmetology and dermatology as well as in the treatment of contaminated surgerical wounds and thermal injuries.

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Assessment of antimicrobial activity of polyazolidinammonium modified by iodine hydrate ions depending on its physical and chemical characteristics.

Year: 2015, volume 11 Issue: №3 Pages: 255-257
Heading: Microbiology Article type: Original article
Authors: Vakaraeva M.M., Uljanov V.Yu., Nechaeva O.V., Luneva I.0., Tikhomirova E.l., Shapoval O.G., Zayarskiy D.A.
Organization: Saratov State Medical University, Saratov state university
Summary:

Aim: to study antimicrobial activity of variants of polymeric compound polyazolidinammonium modified by iodine hydrate-ions (PAAG-M), depending on the polymeric chain length and concentration of the iodine hydrate-ions against standard strains of gram-positive and gram-negative bacteria as well as microscopic fungi. Materials and methods. Minimum inhibitory concentrations of all variants of the polymeric compound for tested microbial strains have been determined by using serial dilution method. Results. High sensitivity of Eschehchia coli 113-13 and Pseudomonas aeruginosa ATCC 27853 was shown to the variants of PAAG-M with molecular weight

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THE USING OF SYNTATIC ANTIOXIDANTS FOR PRESERVING OF BACTERIUM VITALITY WITH STRESS ABIOTIC FACTORS EFFECT (АНГЛ)

Year: 2006, volume 2 Issue: №3 Pages: 22-27
Heading: Microbiology Article type: Original article
Authors: O.V. Nechaeva, E.I. Tikhomirova
Organization: Saratov State Medical University, Saratov National Research University n.a. N. G. Chernyshevsky
Summary:

The influence of synthetical compound with antioxidative activity on increasing of vitality gram-positive and gram-negative microorganisms under stressful action of different antibiotical factors was shown.

Keywords: antioxidant