Saratov JOURNAL of Medical and Scientific Research

Yuri Gagarin State Technical University of Saratov

Evaluation of the wound healing capacity of the gel form of the preparation "Mellisol" on the model of experimental planar wounds

Year: 2017, volume 13 Issue: №3 Pages: 635-640
Heading: Dermatovenerology Article type: Original article
Authors: Myl'nikov A.M., Nechaeva O.V., Zayarskiy D.A., Bespalova N.V., Mudrak D.A., Navolokin N.A.
Organization: Saratov State Medical University, Yuri Gagarin State Technical University of Saratov
Summary:

Objective: to study the wound healing activity of the gel form of the "Mellisol" preparation containing nanoaggregates of flavonoids stabilized with polyazolidine ammonium modified with iodine hydrate ions on a model of experimental planar wounds of rats. Material and Methods. An investigation of the wound healing activity of the gel form of the "Mellisol" preparation was carried out in vivo in 18 white laboratory rats of the Wistar line. Simulation of experimental planar wounds was performed according to the standard procedure described in the preclinical testing manual. Results. In the course of the studies, it was established that a statistically significant (p=0.045) acceleration of healing on the 12th day of the experiment occurred in animals of the experimental group on whose wounds the gel form of the cosmetic means "Mellisol" was applied, and the complete healing of the wound defect occurred on the 21st day after operation. Conclusion. The gel form of the preparation "Mellisol" is characterized by a high level of antimicrobial and regenerative activity reliably reducing the healing time for planar wounds without the development of microbial complications.

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Different reaction of core histones H2A and H2B to the red laser radiation

Year: 2017, volume 13 Issue: №3 Pages: 469-472
Heading: Physiology and Pathophysiology Article type: Original article
Authors: Brill G.E., Egorova A.V., Bugaeva I.O., Ushakova O.V., Matyushkina O.L.
Organization: Saratov State Medical University, Yuri Gagarin State Technical University of Saratov
Summary:

Aim: to investigate the influence of red laser irradiation on the processes of self-assembly of core histones H2A and H2B. Material and Methods. Solutions of human histone proteins were used in the work. Self-assembly was studied by the method of wedge dehydration. Image facies analysis consisted in their qualitative characterization and calculation of quantitative indicators with subsequent statistical processing. Results. It was established that linearly polarized laser light of the red region of the spectrum (A=660 nm, 1 J/cm2) significantly modifies the process of self-assembly of core histone H2B, while the structure of the facies of H2A histone changing to a lesser extent. Conclusion. Red laser radiation influences on the on the processes of self-assembly of core histones H2A and H2B. There is a differential sensitivity of different classes of histones to laser action. Histone proteins used in the experiments are present in the form of aqueous salt solutions. Red light realizes the effect seems to be due to the formation of singlet oxygen by direct laser excitation of molecular oxygen.

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Development of ophthalmic training simulator for cataract surgery

Year: 2017, volume 13 Issue: №2 Pages: 417-420
Heading: Ophtalmology Article type: Original article
Authors: Kumova S.V., Lunev I.A., Gasparyan E.A., Virsta A.M., Batishcheva Yu.S., Kamenskikh T.G., Dolinina O.N.
Organization: Saratov State Medical University, Saratov state university, Yuri Gagarin State Technical University of Saratov
Summary:

Purpose: development of interactive simulator for the development of phacoemulsification. Material and Methods. Material and methods: the Software developed by the authors ophthalmic virtual simulator has several modules that can also be complemented by depending on put before the students the task. Testing the virtual simulator "Phacoemulsification" held at the Department of ophthalmology with the participation of 20 interns and 18 residents. Results. The development of residents and interns of ophthalmological profile of the theoretical foundations of cataract surgery using developed by the authors of the publication virtual interactive simulator demonstrated the effectiveness of this technique in comparison with passive viewing of video recording operations and the familiarity with the course of phacoemulsification in the operating room. Conclusion. The application of the developed simulator with the purpose of optimization of educational process of residents and interns of ophthalmic profile is a very effective tool in the world today.

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Clinical and immunological parallels in the diagnosis of primary open-angle glaucoma.

Year: 2017, volume 13 Issue: №2 Pages: 378-383
Heading: Ophtalmology Article type: Original article
Authors: Kamenskikh I.D., Kamenskikh T.G., Kolbenev I.O., Zakharova N.B., Kozletsov A.P., Tuchin V.V.
Organization: Saratov State Medical University, Saratov state university, Yuri Gagarin State Technical University of Saratov
Summary:

The purpose of study is an assessment of the informative value of various diagnostic parameters in monitoring and prognosis of the state of the visual organ in patients with primary open-angle glaucoma. Material and methods. 216 patients (216 eyes) were examined. 126 patients (126 eyes) with the diagnosis of primary open-angle glaucoma I, II or III stage were included in the main group (I), and 90 patients (90 eyes) without ophthalmologic pathology were included in the control group (II). All patients underwent complex examination. Laser confocal scanning tomography for the analysis of the optic disc morphology was performed using a Heidelberg Retina Tomograph II from Heidelberg Engineering (Germany). Evaluation of the field of view was carried out using the computer static perimeter Oculus twinfield-2 (Germany). Electrophysiological studies (apparatus Roland Consult, Germany) included: the study of visual cortical potentials on the outbreak, the pattern-electroretinogram, photopic negative response. Neurotrophic brain factor, ciliary neurotrophic growth factor and protein NF-200, S-100 protein, monocyte chemoattractant, vascular endothelial growth factor were determined in the serum. Results. An increase in the concentration of the S-100 protein with progression of POAG testifies to the existence of parallels between apoptosis of retinal ganglion cells and systemic neurodegenera-tion affecting the central nervous system as a whole. The importance of electrophysiological research methods, which give information about the third neuron of the visual pathway, has been confirmed. Pattern-electroretinogram, photopic negative response most reflect abnormalities in the work of ganglion cells of the retina, including before the appearance of structural changes in the optic disc and the retina. Conclusion. The interrelation of dynamics of functional, morphological and immunological criteria of progression of POAG is revealed, which allows to predict the further course of the pathological process. Since the study involved patients with IOP target values, it is important not only to maintain IOP on target values, but also to carry out neuroprotection.

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Computer analysis of epiretinal membrane parameters

Year: 2017, volume 13 Issue: №2 Pages: 350-358
Heading: Ophtalmology Article type: Original article
Authors: Daurov S.K., Dolinina O.N., Kamenskikh T.G., Batischeva Yu.S., Kolbenev I.O., Andreychenko O.A., Potemkin S.A., Proskudin R.A.
Organization: Yuri Gagarin State Technical University of Saratov, Saratov State Medical University
Summary:

Objective: to develop algorithms of processing of video images of optical slices of the eye retina to quantify the degree of folding of the epiretinal membranes and of the central fossa. Material and methods. The object of the study was the video image of the retina obtained by optical coherence tomography. To develop methods of determining the degree of folding epiretinal membrane was formed mathematical model of the profile consisting of a base profile (low frequency component) and folding (high frequency part). Results. There had been developed two alternative methods of estimation of folding epiretinal membrane: retinal-averaging method and the method using the wavelet transform. The algorithm of geometrical parameters of the central fossa: the height, width and line shape. These algorithms are implemented in a software system. Conclusion. The practical application of the developed system showed its adequacy, as well as an introduction into medical practice the use of quantitative estimates of some parameters of the retina condition.

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Study of Zn-hydroxyapatite crystals formation and growth on dentine base at ultrasonic influence

Year: 2017, volume 13 Issue: №2 Pages: 260-265
Heading: Stomatology Article type: Original article
Authors: Kazakova L.N., Tereschuk O.S., Kuligin A.V., Suetenkov D.Ye., Pichkhidze S.Ya., Garievskaya A.V.
Organization: Saratov State Medical University, Yuri Gagarin State Technical University of Saratov
Summary:

The objective: the study analyses the features of spontaneous growth of hydroxyapatite crystals on dentinal substrate using (by the use of) artificially grown Zn-hydroxyapatite combined with ultrasonic treatment. Material and Methods. A dynamic study of dentinal substrate structure was conducted in a certain time interval after standard primary surface treatment of dentin micro-sections, divided into control and experimental groups. Dentin surfaces in the second group were covered by finely dispersed Zn-hydroxyapatite in combination with ultrasonic treatment. The investigation was performed by use of analytical complex based on scanning electron microscope with high resolution SEM Mira II LMU (Tescan) and energy dispersive analysis system. Results. There were revealed the structural changes of dentinal substrate surface overtime period due to directional growth of Zn-hydroxyapatite crystals. Conclusion. The conducted study showed the possibility of formation of the stable structures in the dentinal substrate when using Zn-hydroxyapatite in combination with ultrasonic treatment.

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