Saratov JOURNAL of Medical and Scientific Research

Urology

The value of tumor markers in prognos-ing the outcomes of treatment of patients with bladder cancer

Year: 2018, volume 14 Issue: №4 Pages: 661-666
Heading: Urology Article type: Review
Authors: Ponukalin A.N., Zakharova N.B., Skriptsova S.A., Fomkin R.N., Chekhonatskaya M.L.
Organization: Saratov State Medical University n.a. V I. Razumovsky, Scientific Research Institute of Fundamental and Clinical Uronephrology, Saratov State Medical University
Summary:

The review analyzes the recent publications of the results of the use of molecular genetic markers of bladder cancer (RMP) in clinical practice. The prospects of application of a series of molecules determined in tumor tissue, serum, urine in the diagnosis of RMP are presented. One ofthe most promising areas of finding a marker forthe diagnosis and control of treatment of RMP in recent years are the study ofthe proteomic composition of urine.

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Biomarkers of renal damage and immunohistochemical detection of oncogenesis in the surgical treatment of renal cell carcinoma

Year: 2018, volume 14 Issue: №3 Pages: 420-426
Heading: Urology Article type: Original article
Authors: Tarasenko A.l., Rossolovsky A.N., Berezinets O.L., Ponukalin A.N., Medvedeva A.M., Zakharova N.B., Popkov V.M., Maslyakova G.N.
Organization: Saratov State Medical University, I.M. Sechenov First Moscow State Medical University
Summary:

Objective: to assess the possibility of using markers of renal injury and carcinogenesis as additional criteria for the choice of the method of surgical treatment of RCC and to minimize the risk of development and progression of kidney injury. Material and Methods. 60 patients with morphologically confirmed RCC T1-3M0N0 operated on in the volume of open nephrectomy or laparoscopic nephrectomy or laparoscopic resection and the kidney were examined. Patients were divided into 3 groups: group 1: patients after nephrectomy performed for kidney cancer using traditional surgical technique, group 2: patients after laparoscopic nephrectomy and group 3: after laparoscopic resection of kidney tumor. Before surgical operation, on the 5-7 day and after 1 month, all patients were examined by concentrations of urinary excreted lipocalin 2 (NGAL), and serum concentrations of IL-18, MMP-9 and IGF-1. Renal function was estimated using the calculated GFR equation (CKD-EPI 2009) based on serum creatinine level. Immunohistochemical study of histological preparations of distant tumors on proliferation antibodies (Ki-67, PCNA), apoptosis (BAX, Bcl-2) was performed. Differences in clinical data and clinical variables were compared using Spearman rank correlations and t-test. Results. The studied indices of renal damage of IL-18, NGAL, MMP-9 and IGF-1 both in the early (5-7 days) and later (1 month) postoperative period showed a correlation between the surgical intervention method and the degree of tumor cell differentiation. Conclusion. The levels of biomolecular markers of renal damage (IL-18, NGAL, MMP-9 and IGF-1) correlate with the degree of differentiation in thetumoral cells and functional activity of the remaining renal parenchyma in the postoperative period, but also depend on the individual characteristics of the patient's body. A certain level of expression of MMP-9 and IL-18 with a small tumor size can be a guide to determine the indications for the choice of organ-preserving surgery in patients.

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Complications after different surgical methods of setting polypropylene suburethral meshes in women with stress urinary incontinence

Year: 2018, volume 14 Issue: №2 Pages: 277-280
Heading: Urology Article type: Original article
Authors: Eisenakh I.A., Korotkevich O.S., Mozes V.G., Vlasova V.V.
Organization: Kemerovo State Medical University, Novokuznetsk State Clinical Hospital № 1
Summary:

The aim: to determine the complication's rate, based on a seven-year experience, after the stress urinary incontinence correction in women with different methods of setting suburethral polypropylene meshes. Material and Methods. The analysis of seven years of experience of surgical correction with different methods of setting suburethral meshes in 1260 women with stress urinary incontinence; obturator fixation the mesh inside-outside and outside-inside: supra-pubic fixation the mesh; non-trocar fixation the mesh type mini-sling was carried out. Results. The greatest frequency of complications were identified in the first two years of implementation the setting of suburethral meshes, after that the complication's rate decreased progressively and it was in the range 7-8% the last three years. The frequency of early operative complications at a different setting of suburethral meshes had no statistically significant differences, but there were more the statistically significant intraoperative and mesh-associated complications at suprapubic fixation. Conclusion. The study confirms that the installation of suburethral meshes by the method of suprapubic fixation of mesh is associated with a higher incidence of complications and should be performed only in the target group of patients who are not suitable for surgical correction in safer ways.

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Endoscopic correction of obstructive complications after HIFU-ablation of the prostate with holmium laser

Year: 2018, volume 14 Issue: №2 Pages: 272-277
Heading: Urology Article type: Original article
Authors: Popkov V.M., Fomkin R.N., Shatylko T.V.
Organization: Saratov State Medical University
Summary:

Purpose: to improve the results of treatment of the obstructive complications (an urethra stenosis, a bladder neck sclerosis) caused by application of the high-intensity focused ultrasonic ablation (HIFU) concerning treatment of the localized prostate cancer. Material and Methods. An object of the research included 41 patients, with the infravesical obstruction (IVO) which has arisen in the postoperative period to which elimination the holmium laser is used. Results. The bladder neck was the most frequent place of obstruction. Average time before emergence of the first episode of IVO: 8.2±1.3 months. Distinctions on time before development of an episode of IVO depending on the fact of carrying out TURP before HIFU not confirmed (p=0.440). More advanced age for a moment HIFU-ablation has been associated with much higher frequency of IVO (p=0.021). Considerably smaller frequency repeated was observed by IVO at a larger ratio transurethral resection to the initial volume of a prostate (p=0.031). Duration of a laser endoscopic ure-throtomy was 27 [10-70] minutes, serious perioperative or postoperative complications were not determined. Average term before removal of a catheter of Foley: 3 [1-5] days, hospitalization duration included 4 [3-6] days. After treatment considerable improvement on indicators of the maximum speed of a stream of urine (Qmax) and volume of residual urine was observed. The total score according to the questionnaires of IPSS and QoL has considerably improved, significant changes on symptoms of accumulation are noted. After the first holmium laser urethrotomy of 80.4% of patients have noted satisfactory results without recurrence of IVO; 19.6% of patients were required a repeated laser endoscopic urethrotomy. Recurrence term after the first laser urethrotomy is on average equal to 2.5 months. Conclusion. The endoscopic urethrotomy by means of the holmium laser is safe, effective and minimum invasive type of treatment of a stenosis of a neck of the bladder / urethra after ablative cancer therapy of prostate. The advantages are the minimum of traumas of surrounding tissues and preservation of the mechanism of deduction of urine.

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The prospects for application of biomarker "vascular endothelial growth factor" in predicting the treatment outcomes of bladder cancer

Year: 2018, volume 14 Issue: №2 Pages: 268-272
Heading: Urology Article type: Original article
Authors: Zakharova N.B., Ponukalin A.N., Skriptsova S.A.
Organization: Saratov State Medical University
Summary:

Purpose: assessment of the diagnostic value of Vascular Endothelial Growth Factor (VEGF) in predicting the outcome treatment of patients with muscle-invasive bladder cancer. Material and Methods. The study involved 264 people: 204 patients with RMP; 60 people aged 21 to 60 years were the comparison group. Patients with RMP are divided into stages of the disease. With non-muscle-invasive ВС (NIRMP) under TA-1NxMo there were 75 patients. Out of 129 patients with muscle-invasive ВС (MIRE) within the body (pT2aN0M0-T2bN0M0) was diagnosed in 69 of people. In 60 patients, the tumor sprouted paravesical tissue and surrounding organs (rt3a-bn0m0-41; pT36N1M0-11; pT4N1M0-5; T4N1M1-3); 19 patients had metastases to the iliac lymph nodes are installed only after the operation. Metastases were found in three patients out of 19 in addition to the lymphonoduses in the lungs. Tumors had the following gradations: G1-97; G2-37; G3-58. All patients NMIN (n=75) completed a TUR of the bladder wall with the tumor. MERE patients underwent radical cystectomy (n=117), open resection of the bladder (n=12), palliative surgery (n=3). The period of follow-up after surgical treatment was 3.5±0.5years. Quantitative determination of VEGF in blood serum was performed by solid-phase ELISA. Results. Despite the fact that the increase in VEGF level of blood serum in groups of patients with NIMRE and MIRE has significant differences both before and in the postoperative period, it is associated with the aggressiveness of tumor growth, development of metastases and recurrences of the disease. Conclusion. The increase in the content of VEGF serum suitable for use as an indicator: the risk of relapse within the first year after TUR in patients NMIN; development of relapses and metastases in pre and postoperative periods in MERE patients.

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A comprehensive approach to the diagnosis and treatment of straghorn nephrolithiasis

Year: 2018, volume 14 Issue: №1 Pages: 81-86
Heading: Urology Article type: Review
Authors: Chekhonatskaya M.L., Rossolovsky A.N., Kryuchkov I.A., Bobylev D.A.
Organization: Saratov State Medical University
Summary:

A review of the literature is devoted to the most topical diagnostic questions and treatment of patients with urolithia-sis. The role and the latest advances and benefits of minimally invasive surgery were described. Particular attention was paid to the problems of residual calculi and the condition of the renal parenchyma in the perioperative period. The theme of choice of conducting such patients tactics was considered. The actual diagnostic methods and a differentiated approach to the algorithm choice of the examination and treatment of patients with staghorn nephrolithiasis were considered. The review provided a scientometric analysis of research information (documents reference), contained the analytical array of scientific publications. Electronic information resources of scientific electronic library bibliographic databases: eLibrary, PubMed, Clinical Key, Science Direct — were used.

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Physical factors impact on the status of regional hemodynamics and processes of microcirculation in patients with chronic bacterial prostatitis

Year: 2017, volume 13 Issue: №3 Pages: 537-540
Heading: Urology Article type: Original article
Authors: Samoylov A.S., Kulchitskaya D.B., Kolbakhova S.N., Goloborodko E.M., Bokov A.l., Konchugova T.V., Kiyatkin V.A.
Organization: Russian Scientific Center for Medical Rehabilitation and Balneology, State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency
Summary:

The goal of the research is to study the impact of vacuum interference therapy on the state of the regional hemodynamics and the processes of microcirculation among patients with chronic bacterial prostatitis. Material and Meth- ods. The study included 90 patients with average age 32.5 years with chronic bacterial prostatitis in a latent phase of inflammation. The study was conducted on a comparative basis using three randomized groups of patients (n=30), comparable on all criteria, divided depending on the methods applied into two treatment groups and one control group. Besides, the originally assigned basic medication treatment was an obligatory component in all three groups. Results. After therapy course the more significant results in hemodynamic changes were shown in the group of patients who received vacuum-interference therapy. The second group, which received interference currents, there was also positive dynamics in the hemodynamic parameters, but to a smaller extent, while in the control group there were no vivid changes found in the studied parameters. Conclusion. The course treatment using vacuum interference therapy among patients with chronic bacterial prostatitis had a significant positive corrective effect on the system of hemodynamics.

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Physiotherapeutic methods in treating patients with chronic abacterial prostatitis / chronic pelvic pain syndrome: current and development perspectives

Year: 2017, volume 13 Issue: №3 Pages: 532-536
Heading: Urology Article type: Review
Authors: Popkov V.M., Churakov А.А., Dolgov А.В., Rossolovsky A.N., Spirin P.V.
Organization: Scientific Research Institute of Traumatology Orthopedics and Neurosurgery of Saratov State Medical University n.a. V.I. Razumovsky
Summary:

Chronic abacterial prostatitis / chronic pelvic pain syndrome is one of the most common diseases in urological practice. Physical factors are widely used in treatment of this pathology. This literature review is devoted to study of role of physiotherapeutic methods in treatment of chronic abacterial prostatitis and prospects for development of this therapeutic direction.

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Dynamics of angiogenesis and inflammation biomarkers in patients with urolithic disease with obstructive uropathy

Year: 2017, volume 13 Issue: №3 Pages:
Heading: Urology Article type: Original article
Authors: Popkov V.M., Zakharova N.B., Ponukalin A.N., Polozov A.B., Khotko D.N., Khotko A.l., Spirin P.V.
Organization: Scientific Research Institute of Traumatology Orthopedics and Neurosurgery of Saratov State Medical University n.a. V.I. Razumovsky
Summary:

Purpose: to establish the nature of changes in markers of angiogenesis and inflammation in the blood serum and urine in patients with obstruction of the ureteropelvic segment or upper third of the ureter by ureteral stones in different terms of the kidney drainage. Material and Methods. 53 patients with urolithiasis have been examined. The main group consisted of 33 patients with concrements of the upper third of the ureter or renal pelvis-ureteral segment with pres- ent obstructive uropathy. The comparison group consisted of 20 patients with calculi of the kidney without symptoms of obstruction. In the main group patients underwent drainage of the kidney by percutaneous-nephrostomy puncture. Results. Patients of the main group revealed a significant increase in the levels of markers of angiogenesis and inflammation before the kidney drainage. The level of MCP-1 correlated with the level of serum creatinine. In the process of drainage the levels of these markers were decreased as a result of increased GFR. Determination of the concentration of urinary VEGF, IL-8 and MCP-1 in patients may have clinical value in personalized management of patients with obstructive uropathy to determine the optimal terms of the second stage of the surgical intervention. Conclusion. Determination of markers of inflammation and angiogenesis in the urine of patients in conjunction with assessment of renal function by creatinine level is important for determining the term of kidney drainage.

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The influence of premorbid background on the course of urolithiasis

Year: 2017, volume 13 Issue: №2 Pages: 280-283
Heading: Urology Article type: Original article
Authors: Osnovin O.V., Popkov V.M., Fomkina O.A.
Organization: Saratov State Medical University
Summary:

Aim: to analyze the correlation between the unfavorable factors at newborn patients and children and the peculiarities of the course of urolithiasis in adults. Material and Methods. The data were received from questioning of 286 patients and their relatives, analysis of case histories and past medical histories. The material was statistically worked out. Results. The early development of urolithiasis (at patients under 30) was associated with the threat of termination of pregnancy, prematurity, excretory and digestive disorders at childhood in case of severe anamnesis of urolithiasis, and also malnutrition. Bilateral lesions of the urinary system were determined to be associated with artificial feeding and prematurity, digestive diseases and complicated anamnesis of urolithiasis, malnutrition, deprived living conditions and low income. Stones in calyces occurred at patients with different complications at birth. More frequent cases of stone formation in renal pelvis were observed at premature and malnutrition patients and patients with cardiovascular diseases occurred during the childhood. Stones in ureters were considered as a result of respiratory diseases effect. Conclusion. Unfavorable factors occurred at birth and childhood, factors concerned with deprived social conditions and psychological problems influence the onset and the course of urolithiasis.

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