Saratov JOURNAL of Medical and Scientific Research

biomarker

Dynamics of angiogenesis and inflammation biomarkers in patients with urolithic disease with obstructive uropathy

Year: 2017, volume 13 Issue: №3 Pages:
Heading: Urology Article type: Original article
Authors: Popkov V.M., Zakharova N.B., Ponukalin A.N., Polozov A.B., Khotko D.N., Khotko A.l., Spirin P.V.
Organization: Scientific Research Institute of Traumatology Orthopedics and Neurosurgery of Saratov State Medical University n.a. V.I. Razumovsky
Summary:

Purpose: to establish the nature of changes in markers of angiogenesis and inflammation in the blood serum and urine in patients with obstruction of the ureteropelvic segment or upper third of the ureter by ureteral stones in different terms of the kidney drainage. Material and Methods. 53 patients with urolithiasis have been examined. The main group consisted of 33 patients with concrements of the upper third of the ureter or renal pelvis-ureteral segment with pres- ent obstructive uropathy. The comparison group consisted of 20 patients with calculi of the kidney without symptoms of obstruction. In the main group patients underwent drainage of the kidney by percutaneous-nephrostomy puncture. Results. Patients of the main group revealed a significant increase in the levels of markers of angiogenesis and inflammation before the kidney drainage. The level of MCP-1 correlated with the level of serum creatinine. In the process of drainage the levels of these markers were decreased as a result of increased GFR. Determination of the concentration of urinary VEGF, IL-8 and MCP-1 in patients may have clinical value in personalized management of patients with obstructive uropathy to determine the optimal terms of the second stage of the surgical intervention. Conclusion. Determination of markers of inflammation and angiogenesis in the urine of patients in conjunction with assessment of renal function by creatinine level is important for determining the term of kidney drainage.

AttachmentSize
2017_03_527-532.pdf378.73 KB

The role of biomarkers in early diagnostics of inflammatory diseases of intestine in newborns

Year: 2017, volume 13 Issue: №2 Pages: 256-259
Heading: Pediatrics Article type: Review
Authors: Chernenkov Yu.V., Pozgaleva N.V. Panina OS.
Organization: Saratov State Medical University
Summary:

Information on noninvasive biomarkers and methods for studying inflammatory bowel diseases in newborns has been summarized. It is based on the analysis of data taken from foreign and Russian literature. The need for additional study of factors affecting the level of fecal protein production in newborns is substantiated.

AttachmentSize
2017_02_256-259.pdf262.79 KB

Diagnostic value of bio-markers in the prognosis of postoperative relapses and metastases in patients with breast cancer

Year: 2017, volume 13 Issue: №2 Pages: 239-244
Heading: Oncology Article type: Original article
Authors: Cheburkayeva M.Yu., Zakharova N.B., Fedorov V.E., Teryoshkina N.E., Gergenreter Yu.S.
Organization: Regional Oncology center №2, Saratov State Medical University
Summary:

Purpose: to improve the diagnosis of relapse and metastasis of breast cancer in the postoperative period. Material and methods. 123 women were examined. Of these, 30 practically healthy women made up a control group, 20 women had benign breast diseases, 73 had breast cancer. The patients with ВС before the surgical intervention were divided into groups according to the stage of the disease. The 1st group consisted of 30 practically healthy women. In the second group — 20 women with fibroadenomas of the breast. In the third group — 33 women with l-ll stages of breast cancer without regional metastases. The 4th group consisted of 22 women with stage II breast cancer with regional metastases, in the 5th group — 18 patients with stage III breast cancer. After surgery, 68 patients were re-examined and divided into 2 analytical groups. The 6th group included women without clinical manifestations of relapses and metastases in the postoperative period — 44 (64.7%). Group 7 consisted of 24 (35.3%) women who had metastasis and local recurrences of the disease in the postoperative period. To determine the content of fetal proteins (Ca-15-3, Ca-125, REA), IL-8 and VEGF, commercial sets of reagents for solid-phase enzyme-linked immunosorbent assays were used. Results. Analysis of the data obtained showed that the most significant increase in serum and plasma VEGF levels occurred in breast cancer patients with clinical signs of metastasis and recurrence of the disease. Of fetal proteins, a significant increase in blood content showed Ca15-3 and CEA. Conclusion. We can assume that in patients with breast cancer only in the postoperative period, the rise in VEGF was associated with the growth of tumor tissue and the development of invasion and metastasis.

AttachmentSize
2017_02_239-244.pdf516.97 KB

The diagnostic importance of biomarkers of angio-genesis and inflammation at patients with prostate diseases

Year: 2016, volume 12 Issue: №4 Pages: 593-598
Heading: Urology Article type: Original article
Authors: Popkov V.M., Zakharova N.B., Ponukalin A.N., Tonkoschkur A.N.
Organization: Saratov State Medical University
Summary:

Purpose: to establish the nature of change of the maintenance of PSA and markers of angiogenesis and inflammation in blood serum at patients with prostate diseases. Material and Methods. 72 patients with the level of the general PSA more than 4 ju/ml were examined. The first group of patients with benign prostatic hyperplasia with histology conclusion and with benign prostatic hyperplasia and prostatic intraepithelial neoplasia of low degree included 29 patients, the second group consisted of 9 patients with prostatic intraepithelial neoplasia of high degree, the third group included 5 patients with the localized prostate cancer, the fourth group consisted of 25 patients with locally advanced prostate cancer, and the fifth group with 6 metastatic prostate cancer patients. Results. At patients with prostate diseases the rise of VEGF level in blood serum occurs with increase of the maintenance of PSA and develops at the precancer stage (the prostatic intraepithelial neoplasia of high degree) and against high concentration of biomarkers of inflammation. Determination of VEGF concentration in blood serum at patients with "a gray zone" of PSA may have clinical value at the personified maintaining of patients with prostate cancer at the stage of pre-biopsy diagnostics. Conclusion. The simultaneous increase of the VEGF content in blood serum and the general PSA at prostate cancer patients is one of characteristics of a progression of tumoral growth.

AttachmentSize
2016_04_593-598.pdf475.7 KB

Magnetic resonance imaging and biomarkers of serum and urine wile diagnostics of kidney cancer

Year: 2016, volume 12 Issue: №1 Pages: 52-56
Heading: Parmacology Article type: Original article
Authors: Nickolsky Yu.Ye., Chekhonatskaya M.L., Zakharova N.B., Popyhova E.B., Ponukalin A.N.
Organization: Saratov State Medical University
Summary:

Purpose: improvement of differential diagnostics of benign and malignant renal tumors basing on complex estimation of the results of MRTand the level of such biomarkers as vascular endothelial growth factor, monocyte chemotactic protein-1 and matrix metalloproteinase-9 in blood serum and urine. Material and Methods. A total of 106 patients including the main group of 60 patients with renal cancer (RC), the group of comparison of 16 patients with benign renal tumors and the control group of 30 practically healthy persons were examined. ELISA was employed for detection of the biomarkers in blood serum and urine. The tumors were diagnosed by MRT Results. The increase of the level of the biomarkers in blood serum and urine was registered independently of the character of neoplastic process; more significant increase was observed in patients with RC, especially at the early stages of the disease. Some peculiarities of changing of the level of the biomarkers depending on the dimensions of malignant tumors were found. Conclusion. At the early stages of RC complex detection of the abovementioned biomarkers in blood serum and urine can serve an additional clinical diagnostic and prognostic criterion.

AttachmentSize
2016_01_052-056.pdf432.05 KB

Natriuretic peptides and Galectin-3 in senile heart failure patients with preserved ejection fraction.

Year: 2015, volume 11 Issue: №1 Pages: 041-046
Heading: Gerontology and geriatrics Article type: Original article
Authors: Malinova L.I., Podbolotov R.A., Povarova T.V., Pletneva G.F.
Organization: Saratov State Medical University, Saratov Road Clinical Hospital, Regional Hospital for War Veterans, Saratov
Summary:

Purpose: to characterize biomarker panel in senile patients with heart failure with preserved ejection fraction (HF-pEF). Material and Methods. 356 senile patients with chronic heart failure with preserved ejection fraction (HFpEF) were examined to form the study sample of 48 male patients without atrium fibrillation, anemia, diabetes mellitus, onco-pathology and missing clinical data. NT-proBNP, galectin-3 (gal-3) and proANT assay was performed by commercially available ELISA kits. Results. In patients without left ventricular (LV) regional wall motion abnormalities (RWMA). gal-3 (2,13 (0,98; 3,50) vs 5.16 (4,34;9.63) ng/mL, p = 0.011) and NT-proBNP (5,59 (1,00; 10.13) vs 32,04 (15,40; 46,18) fmol/mL, p=0,006) levels were significantly lower than in patients with LV RWMA. Contrarily proANP level was lower in patients with LV RWMA (3,24 (2,47; 3,75) vs 1,38 (0,83; 2,29) nmol/mL, p=0.071). proANP was the only biomarker to increase significantly in long livers (7,30 (4,52; 7,63) vs 2,68 (1,88; 3,32) nmol/mL, p=0.006). NT-proBNP positively correlated with glucose level and negatively with hsCRP (R=0,52, p=0,007). Conclusion. Biomarker panel in senile heart failure patients with preserved ejection fraction has characterized: increase in NT-proBNP and galectin-3 is associated with more severe clinical course and presence of regional wall motion abnormalities in senile patients.

AttachmentSize
2015_01_041-046.pdf939.62 KB

Polybiomarker approach in myocardial dysfunction assessment in senile patients

Year: 2015, volume 11 Issue: №1 Pages: 031-036
Heading: Gerontology and geriatrics Article type: Original article
Authors: Malinova L.I., Podbolotov R.A., Povarova T.V., Avdienko I.V.
Organization: Regional Hospital for War Veterans, Saratov, Saratov State Medical University
Summary:

Purpose: to evaluate interaction of obesity, inflammation and myocardial dysfunction in senile myocardial infarction survivors. Material and Methods. Patients aged 70 and older were involved in the study (n=108) and divided into 2 groups according to the history of myocardial infarction (Ml) — 5 years before involvement: senile Ml survivors (n=26) vs senile patients without history of Ml (n=82). Measurements of serum levels of adipokines (leptin, adiponectin), myocardial dysfunction markers (BNP, NT proBNP, proANP, galectin 3) and inflammatory cytokines (TNF, interleukin 6) were performed. Results. Both groups were comparable by age and major clinical characteristics. Ejection fraction was preserved in both groups under the study (67 (64; 70) %vs67 (64; 68) %, p=0,655). Frequency of diastolic dysfunction was comparable in both groups. However it was more severe in Ml survivors. BNP and NT proBNP levels were significantly lower in patients without the history of Ml (p=0,021; 0,004, respectively). On the contrary serum levels of proANP had tendency to increase in patients with the history of Ml, but not significantly: p=0,821. Adiponectin and galectin-3 were significantly higher in patients with the history of Ml (p=0,019and p=0,011). Conclusion. Pathogenetic peculiarities of chronic heart failure with preserved ejection fraction in senile patients with and without myocardial infarction history were revealed. More expedient biomarker panel appropriate for senile patients with probable heart failure should include NT-proBNP, galectin-3 and adiponectin.

AttachmentSize
2015_01_031-036.pdf893.87 KB