Saratov JOURNAL of Medical and Scientific Research

Borodulin V.В.

Saratov State Medical University, Head of Department Bioorganic Chemistry, Professor, Doctor of Medical Science

Association of polymorphism of CYP2D6 and CYP2C9 genes encoding P-450 proteins of cytochrome with arterial hypertension

Year: 2012, volume 8 Issue: №4 Pages: 933-937
Heading: Internal Diseases Article type: Original article
Authors: Borodulin V.B., Shevchenko O.V., Bychkov E.N., Kiselev A.R., Reshetko O.V., Posnenkova O.M., Saratsev A.V., Losev O.E.
Organization: Saratov State Medical University
Summary:

Gene polymorphisms of cytochrome P-450 CYP2 encoding proteins of cytochrome P-450 are essential forantihy-pertensive drugs metabolism. Purpose: We study the associations of functionally defective allele variants of CYP2D6 gene and CYP2C9 gene with the degree of arterial hypertension (AH). Materials and methods: Samples of DNA of leukocytes of blood of 150 patients with AH without the associated clinical conditions (56% of women) at the age of 20-59
years have been investigated. For the study of polymorphism of genes the pharmacogenetic biochip developed in the Institute of Molecular Biology n.a. V. A. Engelgardt has been used. Comparison of frequencies of occurrence of signs has been carried out on the basis of chi-square criterion. Results: It has been revealed that homozygotes by mutant A1075C, C430T alleles of CYP2C9gene and G1934A of CYP2D6 gene have been significantly more common among patients with hypertension III (p=0.01). Conclusion: The research works on genes of system of P-450 cytochrome have important clinical value for rationalization of pharmacotherapy of hypertension. The increased frequency of occurrence of mutant allele of CYP2D6 and CYP2C9 genes in patients with hypertension III requires special attention to the problem of efficiency and safety of application of hypotensive drugs for the patients.

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The role of genetic mutations in development of metabolic disturbances in patients with arterial hypertension

Year: 2012, volume 8 Issue: №3 Pages: 751-756
Heading: Internal Diseases Article type: Original article
Authors: Borodulin V.B., Shevchenko O.V., Bychkov E.N., Reshetko O.V., Kiselev A.R., Posnenkova O.M., Zhelezinskaja N.V., Sarattsev A.V., Losev O.E.
Organization: Saratov State Medical University, Saratov Institute of Cardiology, Russia, First Moscow State Medical University n.a. I.M. Sechenov, Clinical Hospital of Ministry of Internal Affairs of the Russian Federation
Summary:

The article stresses on the study of molecular markers of essential arterial hypertension providing insight into the pathological mechanisms underlying the disease and creation of «genetic passport» for every patient in the future. Objective: To study genetic markers of 5,10-methylenetetrahydrofolate reductase (MTHFR), involved in the metabolism of homocysteine, and N-acetyltransferase 2 (NAT2), ensuring the process of transformation of acetyl-CoAto acetoacetyl-coenzyme A. Methods: The study involved 160 patients with essential hypertension l-lll stages Caucasian (57.5% female.) aged 20-59 years. MTHFR gene polymorphisms and NAT2 in DNA of peripheral blood leukocytes were studied using a set of Wizard Genomic DNA Purification Kit (Promega, USA). Results: It was shown that «slow acetylators» (homozygous for a mutant version of the gene NAT2) were more frequently (p <0.05) determined among patients with hypertension stage 1. It was also revealed the dependence on the presence of hypertension stage mutant allele T (hetero-and homozygous variants) in 677 nucleotide gene MTHFR (r = 0,40, p <0.001). Conclusions: The regularities of the distribution of polymorphic variants of MTHFR and NAT2 gene were found out in hypertensive patients according to the stage of the disease.

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General methods of identification of narcotic substances in cadaveric urine and biochemical indices in blood at positive identification of drug substances

Year: 2011, volume 7 Issue: №3 Pages: 642-646
Heading: Narcology Article type: Original article
Authors: Bychkov E.N., Serkova S.A., Arsentieva L.A., Frantsuzova T.S., Bluvshtein G.A., Borodulin V.B.
Organization: Saratov State Medical University, Saratov Bureau of Forensic Medical Examination
Summary:

The goal of the present paper was to find effective biochemical markers of chronic narcotization. Materials and methods. The results of biochemical research of biological liquids in human organism and chromatographic methods of drug substances identification in cadaveric urine were analyzed. Research of 197 samples of different biologic liquids of 10 persons was carried out. Control was fulfilled on the basis of 135 samples of biological liquids took of 12 persons. Methods of identification of drug substances are presented, chromatographic and spectral characteristics of drug substances are described. Interrelations of biochemical indices at positive study of drug substances identification were conducted. The increased urea metabolic concentration in cadaveric blood of drug addicts was revealed in comparison with control specimens. Results. (Numeral results are given in Table 3). Correct correlation of biochemical markers was not established. Conclusion. The present results suppose continued study of informative reliable biochemical markers of chronic drug intoxication

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Genetic pathogenesis of essential arterial hypertension (the review)

Year: 2011, volume 7 Issue: №1 Pages: 083-087
Heading: Internal Diseases Article type: Review
Authors: Shevchenko O.V., Svistunov А.А., Borodulin V.B., Ruta A.V., Bychkov E.N.
Organization: Saratov State Medical University, First Moscow State Medical University n.a. I.M. Sechenov
Summary:

It is proved that genetic factors may be considered as fundamental in the development of essential arterial hypertension (AH). Important role in this process belongs to the genes whose products are involved in regulating blood pressure - adrenergic, renin-angiotensin-aldosterone, and homocyclic bradykinin systems. These systems are closely connected by serial and parallel chemical reactions that enable to use genetic testing to determine the state of the whole system. Analysis of polymorphic markers of different groups of genes encoding components of the renin-angiotensin-aldosterone system allows to define the role of each of the pathogenic factor of hypertension in the development of the disease

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Comparative study of antibacterial action of iron and copper nanoparticles on clinical Staphylococcus aureus strains

Year: 2010, volume 6 Issue: №1 Pages: 011-014
Heading: Biochemistry Article type: Original article
Authors: I.V. Babushkina, V.B. Borodulin, G.V. Korshunov, D.M. Puchinjan
Organization: Saratov State Medical University, Saratov Scientific Research Institute of Traumatology and Orthopedics
Summary:

Research objective is to study antibacterial action of nanoparticles of iron and copper on polyantibiotically resistant clinical Staphylococcus aureus strains.
Materials and methods include antibacterial action of nanoparticles of copper and iron on 10 Staphylococcus au¬reus strains, isolated from patients with purulent complications stayed in the in-patient department of traumatology and orthopedics. Solutions of powders of iron and copper have been prepared directly before the experiment in concentra¬tion from 0,001 to 1 mg/ml.
it has been revealed that the influence of nanoparticles on growth of clinical strains and the intensity of antibacterial effect depends on the form of nanoparticles, their concentration and action time. concentration of 0,1 mg/ml and 1 mg/ ml of iron nanoparticles has provoked the decrease in quantity of microbe cells from 3 to 34 % (p <0,01). in smaller concentrations the reliable antibacterial effect has not been observed. Antibacterial activity of copper nanoparticles has been expressed in a wide range of concentrations from 0,001 mg/ml to 1 mg/ml, even during short-term action (30 minutes) it has provoked reduction of quantity of the microbe cells grown on the firm nutrient medium, 97-100 % in comparison with the control (p <0,001).
in conclusion it is to point out that copper nanoparticles have more expressed inhibitory effect on growth of clini¬cal strains of golden staphylococcus than iron nanoparticle suspension. inhibition degree depends on superdispersed powder dosage and incubation period

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Biochemical Changes in Saliva of Patients with Chronic Generalized Parodontitis under Combined Action of Alternating Running Magnetic Field and Laser Radiation

Year: 2009, volume 5 Issue: №3 Pages: 390-393
Heading: Stomatology Article type: Original article
Authors: N.V. Bulkina, V.B. Borodulin, Yu.L. Osipova, A.Yu. Kropotina, O.Yu. Guseva, L.V. Bashkova
Organization: Saratov State Medical University
Summary:

Biochemical changes in oral fluid of patients with chronic generalized parodontitis were investigated; the most informative indices were found out, they were used for estimating complex therapy effectiveness by means of low intensive helium — neon laser radiation and alternating running magnetic field

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ДЕЙСТВИЕ СЕЛЕНОРГАНИЧЕСКИХ СОЕДИНЕНИЙ НА ГРАМОТРИЦАТЕЛЬНЫЕ МИКРООРГАНИЗМЫ ВЫДЕЛЕННЫЕ ОТ УРОЛОГИЧЕСКИХ БОЛЬНЫХ (RUS)

Year: 2006, volume 2 Issue: №4 Pages: 98-106
Heading: Microbiology Article type: Original article
Authors: V.B. Borodulin, P.V. Glybochko, A.F. Yurtaeva et al.
Organization: Saratov National Research University n.a. N. G. Chernyshevsky, Saratov State Medical University
Summary:

Action of preparations is investigated: 2-benzoil-3,5-biphenil-selenophen(compaundl), l,5-bi-(2,4-bioxyphenil)-3-selenopentan(compaund2) and l,5-bi-(n-chlorphenil)-3-selenopentan-l,5(eompaund3) on microorganisms allocated from urological patients. Suppression of bacterial growth is revealed. At action by the given preparations on Escherichia coli it is revealed, that the preparation compaund3 considerably suppresses growth of microbic cells Escherichia coli in comparison with preparations of the compaundl and compaund2. At action on Klebsiella oxytoca, it is revealed, that the given preparations intensively slow down, growth of microbic cells, and linear dependence on a doze of preparations is observed.

ИЗУЧЕНИЕ ДЕЙСТВИЯ ФЕРРОМАГНИТНОЙ ЖИДКОСТИ, СОДЕРЖАЩЕЙ СПЛАВ НАНОЧАСТИЦ CU, ZN , FE НА УРОПАТОГЕННЫЕ ШТАММЫ Е. COLI И PS. AERUGINOSA (RUS)

Year: 2006, volume 2 Issue: №4 Pages: 94-97
Heading: Microbiology Article type: Original article
Authors: A.M. Belov, V.B. Borodulin, P.V. Glybochko et al.
Organization: Saratov National Research University n.a. N. G. Chernyshevsky, Saratov State Medical University
Summary:

It has been studied of an alloy nanobody of copper, zinc and iron on E. coli and Ps. aeruginosa, allocated from sicks by urological diseases. The number of colonies of the microorganisms which have been grown on cups with various concentration nanobody of metals in suspension, and change of parameters of biochemical activity of bacteria was considered. The expressed antibacterial action investigated nanobody on E.coli and Ps. aeruginosa has been as a result revealed.

ПАТОГЕНЕТИЧЕСКИЕ МЕХАНИЗМЫ КАМНЕ0БРА30ВАНИЯ В ПОЧКАХ (RUS)

Year: 2006, volume 2 Issue: №4 Pages: 90-94
Heading: Urology Article type: Original article
Authors: V.B. Borodulin, P.V. Glybochko
Organization: Saratov State Medical University
Summary:

In article possible metabolic ways of formation kidney stones of various structure on the uniform biochemical mechanism are considered. Authors offer pathogenetic model of stones bilding in kidneys and consider experimental approaches for the proof of the offered theory.

METHODS OF OSTEOSYNTHESIS OF ARTICULAR FRACTURES INFLUENCE OVER DEVELOPMENT OF DYSTROPHIC DEGENERATIVE JOINT CHANGES

Year: 2008, volume 4 Issue: №3 Pages: 90-94
Heading: Traumatology and Orthopedics Article type: Original article
Authors: O.V. Beidik, T.N. Lukpanova, D.V. Mandrov, M.B. Litvak, S.A. Nemalyaev, V.В. Borodulin, A.A. Steklov, M.A. Shcherbakov
Organization: Saratov State Medical University
Summary:

The authors of article suggest the way of treatment limbs fracture of the using to spoke-shank device for external fixation. Such approach allows to reduce risk ofdevelopmentofcomplications anddystrophic degenerative jointchanges.

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