Saratov JOURNAL of Medical and Scientific Research

Pulin A.A.

Federal Medical and Biophysical Center п.a. A.I. Burnazyan, Head of cryobank, Candidate of Medical Sciences

Effectiveness of autologous cell products derived from adipose tissue for the treatment of severe local radiation injuries

Year: 2014, volume 10 Issue: №4 Pages: 838-844
Heading: radiation medicine Article type: Original article
Authors: Eremin P.S., Pigaleva N.A., Murzabekov M.B., Lebedev V.G., Lazareva N.L., Eremin I.I., Pulin A.A., Osipov A.N., Bushmanov A.Yu., Kotenko K.V.
Organization: State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency
Summary:

Aim: to investigate the dynamics of ulcer surface healing depending on type of radiation ulcer and cell product in murine experimental model of severe local radiation injuries after exposure to X-rays. Material and Methods. Mature Wistar rats were used for experiments (males, weight 180-200 grams). Standard model of severe local radiation injuries was used: X-ray irradiation of animals on a modified RAP100-10 device. Isolation of the stromal-vascular fraction carried out by enzymatic treatment of adipose tissue. Autologous cells transplantation performed on day 20 after irradiation in the case of acute radiation injury model (early radiation ulcers) and day 120 in the case of long-term effects of radiation injury model (chronic radiation ulcers). As a positive control allogeneic MMSC derived from rat bone marrow were used. Results. Application of autologous cell products derived from adipose tissue in animal model of severe local radiation injuries was investigated for the first time. It was shown that usage of stromal vascular fraction of adipose tissue for the treatment of early radiation injuries not only leads to better improvement (as compared with the use of MMSCs), but also pronounced therapeutic effect could be observed at an earlier time. On the model of long-term effects of radiation injuries therapeutic effect was observed for all cell products. Conclusion. Cell-based products derived from adipose tissue are promising material for future research and clinical application for treatment of local radiation injuriesK) Described method allows choosing treatment strategy basing on patient's individual characteristics and the type of local radiation injuries.

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Characteristics of changes in the number of yH2AX and Rad51 protein foci in human skin fibroblasts after prolonged exposure to low-dose rate X-ray radiation

Year: 2014, volume 10 Issue: №4 Pages: 739-743
Heading: Genetics Article type: Original article
Authors: Ozerov I.V., Eremin P.S., Osipov A.N., Eremin I.I., Tsvetkova A.D., Guseva S.S., Ivanova K.Yu., Gavrilenko 0.I., Pustovalova M.V., Smetanina N.M., Grekhova A.K., Lazareva N.L., Pullin A.A., Maksimova О.A., Gordeev A.V., Bushmanov A.Yu., Kotenko K.V.
Organization: Lomonosov Moscow State University, State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency
Summary:

Aim: to compare the repair process of DNA double-strand breaks in mammalian cells after acute versus prolonged exposure to X-ray irradiation with different dose rates. Material and methods. Studies were performed on primary human fibroblasts isolated from skin biopsies of healthy volunteers (women, 29 and 30 years). Cells were irradiated using an X-ray machine RUB RUST-M1 (JSC "Ruselectronics", Moscow, Russia) at 37°C temperature with a dose rate of 400 mGy/min (200 kV, 2*2.4 mA, a filter of 1.5mm AI) or 4 mGy/min (50 kV, 2*0.4 mA, a filter of 1.5 mm AI). Immuno-cytochemical protein staining was utilized for yH2AX and Rad51 foci analysis. Results. Phosphorylated histone H2AX (yH2AX) and the key protein of homologous recombination Rad51 foci formation and disappearance kinetics were investigated simultaneously in primary human dermal fibroblasts after acute and prolonged exposure to X-ray radiation at a same dose. It was shown that the relative yield of yH2AX foci per dose reduces with decrease in dose rate, while the relative yield of Rad51 foci conversely increases. Conclusion. Our findings suggest the fundamental differences in the ratio of non-homologous end joining and homologous recombination DNA repair in acute versus prolonged irradiated cells.