Saratov JOURNAL of Medical and Scientific Research

Macro- and Micromorphology

Dynamics of laser speckle imaging of blood flow and morphological changes in tissues with a full time local ischemia of pancreas

Year: 2014, volume 10 Issue: №4 Pages: 596-600
Heading: Macro- and Micromorphology Article type: Original article
Authors: Alexandrov D.A., Timoshina P.A., Tuchin V.V., Maslyakova G.N., Palatova T.V., Skorokhod A.A., Lukashevich K.A., Yarovoy A.S..
Organization: Saratov State Medical University, Saratov state university
Summary:

The purpose: to establish influence of a full ischemia of different duration and the subsequent reperfusionon pathology development in pancreas of rats by means of laser speckle-visualization and lifetime digital microscopy. Materials and Methods. The work has been performed on 42 white rats of line Wistar in weight of 200-250 Research of properties of a blood-groove was made by means of methods laser Doppler flowmetry, digital biomicroscopy and a method of laser speckle-contrast visualization. Results. After the termination of a 5-minute full ischemia the speed of bloodflow has been increased in 2-3 times, clinic pancreatic necrosis is marked does not develop. After the termination of 20-minute full ischemia the increase in speed of a bloodflow did not occur, there were morphological and clinical signs of pancreatic necrosis. Conclusion, the efficiency of monitoring of microhemodynamics of pancreas in rats by the method of speckle-capillary of full field has been shown. Multidirectional phase of perfusion changes in pancreas have been revealed after reversible infringement of blood supply of different duration.

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Analysis of observations of acute strokes during the period from 2009 till 2012

Summary:

Objective: analysis of autopsy material of the patients who died with clinical diagnosis of stroke. Material and Methods. The protocols of postmortem investigations of 172 patients have been studied. The interested information were added to the questionnaire. Material was processed statistically. Results. It has been determined that 51,74% of dead patients were men and 48,26% were women. 96,9% of dead patients were from age group of 41-50 and 81-90 years respectively. The causes of death of the patients were edema of brain and pneumonia, which developed within 24 hours to 7 days. In 16,28% and 11.05% the death was fixed from 7 to 14 and from 14 to 28 days, respectively. The conclusive clinical diagnosis more commonly consisted of two basic diseases. Conclusion. There is a positive trend for improvement of the quality of diagnosis of stroke, to reduce the number of mistakes in conclusive clinical diagnosis.

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Morphology of bone structures of hip joint in normal state and in dysplastic coxarthrosis

Year: 2014, volume 10 Issue: №3 Pages: 373-377
Heading: Macro- and Micromorphology Article type: Review
Authors: Anisimova Е.А., Yusupov K.S., Anisimov D.I.
Organization: Saratov State Medical University
Summary:

The review of literature concerns the questions of development, structure, function, blood supply and innervation of structures of hip joint. The characteristics of changes of these structures in dysplastic coxarthrosis of various degree of expressiveness has been presented. Radiological and biomechanical features of hip joint have been described in various types of dysplastic coxarthrosis.

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Morphological changes in liver of laboratory animals at prolonged intake of gold nanoparticles

Year: 2014, volume 10 Issue: №2 Pages: 250-256
Heading: Macro- and Micromorphology Article type: Original article
Authors: Pakhomy S.S., Bucharskaya А.В., Navolokin N.A., Maslyakova G.N., Zlobina O.V., Khlebtsov N.G., Khlebtsov B.N., Bogatyrev V.A.
Organization: Saratov State Medical University
Summary:

Aim: to evaluate the severity of morphological changes in the liver of laboratory animals with prolonged intake of pegilated gold nanoparticles of different sizes from 2 to 50 nm. Material and methods. The experiment was performed on 240 albino rats divided into 4 groups. The animals of experimental groups were orally administered the gold nanoparticles in a specific pattern. Results. Morphological changes in liver depend on the particle size and the duration of administration. Developing pathological changes in the liver are reversible, as evidenced by the gradual recovery of the liver structure 14 days after administration. Conclusion. The most significant changes in the liver were found in the experimental groups with a 30-day administration of 2 and 50 nm gold nanoparticles that show the size-dependent nature of their impact.

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The ethmoid sinus in the skull: modern views

Year: 2014, volume 10 Issue: №2 Pages: 245-249
Heading: Macro- and Micromorphology Article type: Review
Authors: Mareev O.V., Mareev G.O., Markeeva M.V., Kuchmin V.N.
Organization: Saratov State Medical University
Summary:

Inflammatory diseases of the paranasal sinuses (SNPs) are one of the most urgent problems of otorhinolaryngology. The number of patients with inflammatory diseases of the UNP remains high in our country and abroad, despite the development and introduction of new advanced methods of treatment. From 15to 36% of patients with sinusitis are treated in the ENT departments. Over the last two decades the SNPs diseases increased more than 10 times in Russia. Therefore the diagnosis and treatment of sinusitis is an actual problem of modern otorhinolaryngology. It is necessary to develop effective, reliable and non-invasive diagnostic methods of sinusitis.

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Evaluation of the effectiveness of a resorbable membrane «Bio-Gidein» in combination with chitosan for the treatment of bone defects

Year: 2014, volume 10 Issue: №2 Pages: 239-245
Heading: Macro- and Micromorphology Article type: Original article
Authors: Kalmin O.V., Nikishin D.V., Volodina Yu.M.
Organization: Penza State University
Summary:

Objective: to evaluate the efficiency of a resorbable membrane «Bio-Gide» in combination with chitosan for guided bone regeneration during the degradation under the experimental conditions. Material and Methods. The histological methods provide the study of tissue samples from 6 adult rabbits from artificial mandible through 14, 28 and 42 days after surgery. In group 1 the treatment was carried out without the use of additional materials, by a blood clot. In group 2 closing of the damaged area was produced by xenopericardial plate «Bio-Gide» in combination with chitosan. Results. It has been established that the conduct of bone wound under a blood clot causes inflammatory response on the 14th day after the operation, to the 28th day inflammation disappears and it practically does not appear on the 42nd day. Closure of the bone defect using xenopericardial plate «Bio-Gide» with chitosan causes lesser inflammatory response. In the long-term period after the surgery inflammatory changes in the implantation can not be detected. Conclusion. Xenopericardial plate «Bio-Gide» in combination with chitosan has a positive impact on the recovery process of the bone defect. Neoplasm of bone occurs, the stage of development of cartilage is shortened. At the end of the experiment period xenopericardial plate is not detected indicating the high rate of its biodegradation.

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Stereotopometric characteristics of typical variability of angular parameters of cerebral skull and their connection with basilar angle

Year: 2014, volume 10 Issue: №2 Pages: 233-235
Heading: Macro- and Micromorphology Article type: Original article
Authors: Aleshkina O.Yul, Zagorovskaya Т.М, Polkovova I.A.
Organization: Saratov State Medical University
Summary:

The purpose of the article is to reveal stereotopometric characteristics of typical variability of angular parameters of cerebral skull and their connection with basilar angle. Material and methods.: Stereotopometric research of 100 skulls of adults aged 22-60 years was performed by means of craniostereobasiometer. Stereotopometric method was used to study the coordinates of craniometric points to three perpendicular planes, which have allowed determining size of a basilar angle and angular parameters of the cerebral skull. Resu/fs.Craniotypes by means of size of a basilar angle — flexi-, medio- and platibasilar types of skull base have been established. Comparative analysis of statistics of creniometric points and angular parameters of a cerebral skull at basilar craniotypes has been carried out. Conclusion. It is established that stereotopometric variability of cerebral skull angles, different direction and the degree of their connection with basilar angle depend on the skull base type.

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Variability of cephalometric parameters of men with orthognathic and direct occlusion

Year: 2014, volume 10 Issue: №1 Pages: 52-55
Heading: Macro- and Micromorphology Article type: Original article
Authors: Sheludko S.N., Muzurova L.V., Konnov V.V., Mikheeva M.V.
Organization: Saratov State Medical University
Summary:

The aim: to study the variability of morphometric parameters of the head of men of 21-35 years old, with orthognathic and direct occlusion and identify their differences. Material and Methods. Cephalometry performed in 93 men aged 21 to 35 years from the Saratov region. Distribution groups conducted for the type of physiological occlusion: orthognathic (n=61) and direct (n=32). To determine the parameters used cephalometric anthropometric standard set of tools. On the head cephalometric points were determined between which measured morphometric parameters of the cranial and facial departments, as well as parameters of soft tissue structures. Results. In men with physiological occlusion parameters having statistically significant differences in orthognathic and direct bite were identified: head length, morphological face height, the height of nasal and maxillary division, mandibular body height, width of the face, the angular width of the face. Average values of these parameters are more prevalent in men who have a direct bite.
Conclusion. The data obtained should be considered during orthodontic and orthopaedic treatment, with lectures and practical training in the departments and faculties of the human anatomy with the dental profile.

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Pathomorphological cardial changes in the sudden infant death syndrome

Year: 2014, volume 10 Issue: №1 Pages: 48-52
Heading: Macro- and Micromorphology Article type: Original article
Authors: Rozumny D.V.
Organization: Samara State Medical University
Summary:

In order to establish the relationship between the timing of fetal obliteration of communication and expression remodeling of the heart chambers, as one of the possible causative factors of SIDS, the selected material has been based on macromorphometric study. Material included 43 hearts from infants who died of SIDS. The control group consisted of 48 hearts of children suddenly dead from aspiration of gastric contents and infectious disease. The methods composed a macroscopic examination of the heart by the method of separate weighing of the complex morphometric studies, histological examination (used the following stains: hematoxylin and eosin, picrofuchsin by Van Gieson, Nissl's stain), immunohistochemical method using markers of neurofilament. The results revealed that in children who died of SIDS, obliteration of the fetal communication occurs essentially in the late period —to the age of 8 months, and the mass of the left and right ventricles of the heart exceeds the age limit of normal due to the prevalence of ventricular muscle mass and hypertrophy of cardiomyocytes. In all parts of the heart subendocardial fibrosis in the ventricles — pronounced interstitial fibrosis has been observed. In the interatrial septum at the location at the oval window morphometric study resulted in significant predominance area percentage of connective tissue in relation to the muscle tissue. On histological sections elements of the nervous tissue — randomly arranged nerve ganglia with degenerative changes and nerve fibers were identified. Conclusion. Finally, it should be noted that the growth of cardiosclerosis in all parts of the heart in newborns and infants, who died suddenly from SIDS, extends the terms of obliteration of fetal communications, and degenerative changes in the nerve ganglia that lead to the disturbance of supply and distribution of nerve impulses that pass through them. These factors contribute to the development of hemodynamic disturbances and may be important in the development of sudden death in children.

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The study of changes of biophysical properties of red blood cells in storage in erythrocyte-containing solutions using atomic force microscopy

Year: 2014, volume 10 Issue: №1 Pages: 44-48
Heading: Macro- and Micromorphology Article type: Original article
Authors: Lamzin I.M., Khayrullin R.М.
Organization: Ulyanovsk State University
Summary:

Currently the departments of quality control of blood centers evaluate the suitability for clinical use of erythrocyte-containing solutions indirectly by routine methods of the determination of hemoglobin concentration, hematocrit level, or hemolysis at the end of the period of storing. These methods can not directly characterize the state of membranes of preserved erythrocytes. The aim of the work is to study the changes of elasticity and surface potential of membranes of red blood cells of erythrocyte-containing solutions in storage for 35 days. Material and Methods. Two series of dry cytological preparations (smears) of erythrocytes have been investigated. The first group consisted of 8 samples of erythrocyte-containing solutions prepared on the day of preservation, and the second group included 20 samples prepared after the long term storage for 35 days at t=+4°C. Blood was stored in bags of «Baxter» company (USA) conserving with «CPDA-1». For atomic force microscopy five typical erythrocytes were selected and their elasticity was measured at 9 points of the membrane of each erythrocyte. Total number of measurements was 1296. Results. Average value of Young's modulus of the first group of samples was 1,81±0,02 (M±m) KPa. The second group showed 3,22±0,02 KPa statistically higher (p

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