Saratov JOURNAL of Medical and Scientific Research

Microbiology

Effectiveness evaluation of application of niosomal gel "Regenerin" in the treatment of chemical burns of cornea

Year: 2017, volume 13 Issue: №2 Pages: 216-220
Heading: Microbiology Article type: Original article
Authors: Bazikov I.A., Botasheva V.S., Kalinkina N.I., Maltsev A.N., Kostyukova N.Yu., Domenyuk D. A.
Organization: Stavropol State Medical University
Summary:

The aim of the work was to study the effectiveness of using the ophthalmic gel "Regenerin" in the treatment of a simulated chemical burn of the cornea in an experiment. Material and Methods. The chemical composition of the drug "Regenerin" was studied by mass spectrometry The regenerative action of the niosomal gel, including low molecular weight peptides extracted from placental cells and encapsulated, was then studied in 40 organisms of 20 rabbits of the chinchilla breed with a 10% solution of caustic soda. The subjects of the study are divided equally into two groups: in the experimental group, the treatment was carried out by applying a gel to the burn surface, the therapy was not performed in the control group. Results. The result of treatment with the "Regenerin" Ophthalmic Gel is a significant reduction in the timing of reparative regeneration in the animals of the experimental group due to increased metabolism and corneal neovascularization processes. Conclusion. The combined effect of these mechanisms, manifested in the arrest of edema, resorption of inflammatory infiltrates, normalization of the integrity of the endothelium, epithelial layer and basal membrane leads to completely restoring the histological structure of the cornea on the seventh day.

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Effectiveness evaluation of application of niosomal gel "Regenerin" in the treatment of chemical burns of cornea

Year: 2017, volume 13 Issue: №2 Pages: 216-220
Heading: Microbiology Article type: Original article
Authors: Bazikov I.A., Botasheva V.S., Kalinkina N.I., Maltsev A.N., Kostyukova N.Yu., Domenyuk D. A.
Organization: Stavropol State Medical University
Summary:

The aim of the work was to study the effectiveness of using the ophthalmic gel "Regenerin" in the treatment of a simulated chemical burn of the cornea in an experiment. Material and Methods. The chemical composition of the drug "Regenerin" was studied by mass spectrometry The regenerative action of the niosomal gel, including low molecular weight peptides extracted from placental cells and encapsulated, was then studied in 40 organisms of 20 rabbits of the chinchilla breed with a 10% solution of caustic soda. The subjects of the study are divided equally into two groups: in the experimental group, the treatment was carried out by applying a gel to the burn surface, the therapy was not performed in the control group. Results. The result of treatment with the "Regenerin" Ophthalmic Gel is a significant reduction in the timing of reparative regeneration in the animals of the experimental group due to increased metabolism and corneal neovascularization processes. Conclusion. The combined effect of these mechanisms, manifested in the arrest of edema, resorption of inflammatory infiltrates, normalization of the integrity of the endothelium, epithelial layer and basal membrane leads to completely restoring the histological structure of the cornea on the seventh day.

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Antimicrobial activity of herbal extracts from Sedum maximum (L.) Hoffm., Sedum telephium L. received by different methods

Year: 2017, volume 13 Issue: №2 Pages: 213-216
Heading: Microbiology Article type: Original article
Authors: Raikova S.V., Durnova N.A., Prikhodko V.V., Nemolyaeva E.K., Plastun V.O.
Organization: Saratov State Medical University
Summary:

The main goal of investigation was antimicrobial activity of herbal extracts from Sedum maximum (L.) Hoffm., Sedum telephium L. received by different methods. Material and Methods. Extractions from the purification of S. maximum (L.) Hoffm. and S. telephium L. obtained by different methods were the object of study. Staphylococcus aureus ATCC 6538P, Pseudomonas aeruginosa ATCC 27835, Escherichia coli ATCC 25922, Candida albicans ATCC 13108 were used as test cultures. Results. The minimum inhibitory concentrations of the investigated flavonoid-containing aqueous solutions of alcohol extracts of the purification were determined for the following test cultures: S. aureus, P. aeruginosa, E. coli. Conclusion. The antibacterial effect of the studied extracts on all strains of bacteria taken in the experiment was detected. The action is most pronounced in relation to S. aureus. Antimycotic activity was not detected.

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The microecological and immunological problems of persistention of Helicobacter pylori

Year: 2016, volume 12 Issue: №1 Pages: 19-26
Heading: Microbiology Article type: Review
Authors: Belova O.L., Schelekhova T.V., Belova I.M.
Organization: Saratov State Medical University
Summary:

The interrelation of persistence of Helicobacter pylori in stomach with innate and adaptive immunity of human has been discussed. The analysis of the mechanisms of immunological recognition of the antigens of this microorganism and response to them has been performed. It has been considered, that the development and course of gastritis and ulcer in human with Helicobacter pylori stipulate for inadequate reactions of immune system and common reactivity by the individual organism in presence of Helicobacter pylori in mucus of stomach.

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Assessment of antimicrobial activity of polyazolidinammonium modified by iodine hydrate ions depending on its physical and chemical characteristics.

Year: 2015, volume 11 Issue: №3 Pages: 255-257
Heading: Microbiology Article type: Original article
Authors: Vakaraeva M.M., Uljanov V.Yu., Nechaeva O.V., Luneva I.0., Tikhomirova E.l., Shapoval O.G., Zayarskiy D.A.
Organization: Saratov State Medical University, Saratov state university
Summary:

Aim: to study antimicrobial activity of variants of polymeric compound polyazolidinammonium modified by iodine hydrate-ions (PAAG-M), depending on the polymeric chain length and concentration of the iodine hydrate-ions against standard strains of gram-positive and gram-negative bacteria as well as microscopic fungi. Materials and methods. Minimum inhibitory concentrations of all variants of the polymeric compound for tested microbial strains have been determined by using serial dilution method. Results. High sensitivity of Eschehchia coli 113-13 and Pseudomonas aeruginosa ATCC 27853 was shown to the variants of PAAG-M with molecular weight

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The study of antimicrobial activity of stonecrop extracts (Sedum maximum (L.) Hoffm., S. telephium I.)

Year: 2013, volume 9 Issue: №4 Pages: 640-643
Heading: Microbiology Article type: Original article
Authors: Plastun V.0., Raikova S.V., Durnova N.A., Shub G.M., Komarova E.A.
Organization: Saratov State Medical University
Summary:

The purpose of the work is to investigate the antimicrobial activity of aqueous alcoholic extract of large stonecrop and purple stonecrop. Material and Methods. The effects of large stonecrop (Sedum maximum (L.) Hoffm.) and purple stonecrop (S. telepium L.) on aqueous solution of alcoholic extracts on Staphylococcus aureus АТС С 209R, Pseu-domonas aeruginosa ATCC 27835, Escherichia coli ATCC 25922 have been studied by the method of double serial dilution followed by seeding on solid culture media. As a result of the work the minimum inhibitory concentrations for all extracts to all the studied cultures have been found. Stonecrop extracts have bactericidal action. Conclusion. Antimicrobial activity has been determined for the extracts of both species for all researched samples of microbial strains.

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Effect of low-intensity electromagnetic radiation on structurization properties of bacterial lipopolysaccharide

Year: 2013, volume 9 Issue: №4 Pages: 637-640
Heading: Microbiology Article type: Original article
Authors: Brill G.E., Egorova А.V., Bugaeva I.О, Dubovitsky S.A., Vlaskin S.V.
Organization: Research and production firm “Telemak”, Russia, Saratov, Saratov State Medical University
Summary:

Purpose: to investigate the effects of low-intensity electromagnetic radiation on the process of dehydration self-organization of bacterial lipopolysaccharide. Materials and Methods. The method of wedge dehydration has been used to study the structure formation of bacterial lipopolysaccharide. Image-phases analysis included their qualitative characteristics, as well as the calculation of quantitative indicators, followed by statistical analysis. Results. UHF-Radiation (1GHz, 0,1 uW/cm2, 10 min) has led to the changes in the suspension system of the LPS-saline reflected in the kinetics of structure formation. Conclusion. 1 GHz corresponds to the natural frequency of oscillation of water clusters and, presumably, the effect of UHF on structure of LPS mediates through the changes in water-salt environment. Under these conditions, properties of water molecules of hydration and possibly the properties of hydrophobic and hydrophilic regions in the molecule of LPS, which can affect the ability of toxin molecules to form aggregates change. Therefore the lipopolysaccharide structure modification may result in the change of its toxic properties.

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Electromagnetic radiation influence on clinical course of experimental wound infection

Year: 2010, volume 6 Issue: №3 Pages: 500-503
Heading: Microbiology Article type: Original article
Authors: Pronina Е.А., Raykova S.B., Shvidenko I.G., Shub G.M.
Organization: Saratov State Medical University
Summary:

The article gives close attention to the study of electromagnetic radiation influence (EMR) at the frequency of molecular spectrum absorption and radiation (MSAR) of nitric oxide (150 GHz) and atmospheric oxygen (129 GHz) on the clinical course of experimental wound infection caused by antibiotic-sensitive and antibiotic-resistant strains of Pseudomonas aeruginosa. The panoramic spectrometric measuring complex, developed in Saratov Scientific Research Institute of Measuring Equipment was used while carrying out the research. Electromagnetic vibrations of extremely high frequencies were stimulated in this complex imitating the atmospheric oxygen and nitric oxide absorption and radiation molecular spectrum structure. The experiments proved the fact that exposure to radiation at the frequency of molecular spectrum absorption and radiation (MSAR) of nitric oxide and atmospheric oxygen had positive impact on the course of traumatic process

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Elective course material (Microbial communities)

Year: 2010, volume 6 Issue: №2 Pages: 245-247
Heading: Microbiology Article type: Original article
Authors: G.M. Shub, I.G. Shvidenko, E.A. Pronina, N.V. Beloborodova
Organization: Saratov State Medical University
Summary:

This article gives an outline of data concerning formation and functioning peculiarities of microbial communities in the form of biofilms. Peculiarities of metabolic processes and virulent properties of bacteria existing in biofilms which are connected to those processes are highlighted in the article. The necessity of taking into account these peculiarities when carrying out antibacterial therapy is also stated

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Doxorubicine and antibiotics influence on dynamics of population development of Staphylococcus aureus and Escherichia coli

Year: 2010, volume 6 Issue: №1 Pages: 040-042
Heading: Microbiology Article type: Original article
Authors: O.G. Shapoval
Organization: Saratov State Medical University
Summary:

Infectious complications in oncologic patients often occur as a result of antitumor therapy that demands the prescription of antimicrobic preparations. one of the main infectious agents in oncology is Staphylococci and Escherichia coli. The aim of this work is to study doxorubicine and ceftriaxon influence on dynamics of population development of strains of Staphylococcus aureus, doxorubicine and amikacine - on dynamics of population development of strains of Escherichia coli. Five strains of each type (one-standard and four-clinical) cultivated in meatpeptone broth, which contains non-inhibitory concentrations of experimental preparations. in any period of time after the sowing the measurement of optical density of growing cultures has been carried out. it has been determined, that doxorubicine in non-inhibitory concentrations increases overwhelming effect of these antibiotics on dynamics of population development of experimental strains

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