Saratov JOURNAL of Medical and Scientific Research

Concentration of MMP-9, TNF-a and IL-6 in patients with tumors and tumor-like bone lesions

Year: 2017, volume 13 Issue: №3 Pages: 506-510
Heading: Traumatology and Orthopedics Article type: Original article
Authors: Puchinyan D.M., Pavlenko N.N., Korshunov G.V., Galashina E.A., Gladkova EV.
Organization: Scientific Research Institute of Traumatology Orthopedics and Neurosurgery of Saratov State Medical University n.a. V.I. Razumovsky
Summary:

Aim: to determine the concentration of MMP-9, TNF-a and IL-6 in blood serum of patients with benign and malignant bone tumors and feasibility of cytokine data use for differential diagnostics of the neoplastic process nature. Material and Methods. Levels of matrix metalloproteinase-9 (MMP-9), tumor necrosis factor-a (TNF-a) and interleukin-6 (IL-6) in blood serum were determined by the immunoenzyme method in 64 patients with bone tissue neoplasms (fibrous dysplasia, osteocystoma, giant-cell tumor, osteosarcoma, chondrosarcoma, bone metastases, multiple myeloma). Re-sults. MMP-9 level was heightened in patients suffered from chondrosarcoma and multiple myeloma. TNF-a and IL-6 expression was increased in cases with bone metastases. MMP-9, TNF-a and IL-6 levels were higher in cases with malignant bone neoplasms than in cases with benign bone tumors. Conclusion. MMP-9, TNF-a and IL-6 participate in the neoplastic process pathogenesis directly. Nevertheless it is too early to speak about the diagnostic value of the cytokines in cases with tumorous bone affection.

Bibliography:
1. Salvatore V, Focaroli S, Teti G, et al. Changes in the gene expression of co-cultured human fibroblast cells and osteosarcoma cells: the role of microenvironment. Oncotarget 2015; 6 (30): 28988-28998. doi: 10.18632/oncotarget.4902
2. Adiguzel M, Horozoglu C, Kilicoglu O, et al. MMP-3 gene polymorphisms and Osteosarcoma. Indian J Exp Biol 2016; 54 (3): 175-179
3. Li H, Zhang K, Liu LH, et al. A systematic review of matrix metalloproteinase 9 as a biomarker of survival in patients with osteosarcoma. Tumour Biol 2014; 35 (6): 5487-5491. doi: 10.1007/sl 3277-014-1717-3. Epub 2014 Feb 28
4. Yi WR, Li ZH, Qi BW, et al. Downregulation of IDH2 exacerbates the malignant progression of osteosarcoma cells via increased NF-кВ and MMP-9 activation. Oncol Rep 2016; 35 (4): H.2277-2285. doi: 10.3892/ОГ.2016.4553
5. Li M, Zhu Y, Zhang H, et al. Delivery of inhibitor of growth 4 (ING4) gene significantly inhibits proliferation and invasion and promotes apoptosis of human osteosarcoma cells. Sci Rep 2014; (4): 7380. doi: 10.1038/srep07380
6. Zhou X, Shi X, Ren K, et al. Celecoxib inhibits cell growth and modulates the expression of matrix metalloproteinases in human osteosarcoma MG-63 cell line. Eur Rev Med Pharmacol Sci 2015; 19 (21): 4087-4097
7. Ma Y, Zhu B, Liu X, et al. Inhibition of oleandrin on the proliferation show and invasion of osteosarcoma cells in vitro by suppressing Wnt/p-catenin signaling pathway. J Exp Clin Cancer Res 2015; (34): 115. doi: 10.1186/s13046-015-0232-8
8. Gasiorowska A, Talar-Wojnarowska R, Kaczka A, et al. Subclinical inflammation and endothelial dysfunction in patients with chronic pancreatitis and newly diagnosed pancreatic cancer. Dig Dis Sci 2016; 61 (4): 1121-1129. doi: 10.1007/ s10620-015-3972-6
9. Xiao T, Liao L, Shun C, et al. Determination and significance of nitric oxide and tumor necrosis factor-alpha induced by peripheral blood monocyte in vitro in patients with malignant bone tumors. Hunan Yi Ke Da Xue Xue Bao 1998; 23 (6): 596-598
10. Liu C, Zhao P, Yang Y, et al. Ampelopsin suppresses TNF-a-induced migration and invasion of U20S osteosarcoma cells. Mol Med Rep 2016; 13 (6): 4729-4736. doi: 10.3892/ mmr.2016.5124
11. Кушлинский H.E., Соловьев Ю.Н., Бабкина И. В. и др. Интерлейкин-6 и его растворимый рецептор у больных опухолями костей. Клиническая лабораторная диагностика 2005; (9): 22
12. Халилова B.3., Эфендиев A.M., Ахмедова Г.А., Ибрагимов Э. Э. Интерлейкин-6 и фактор некроза опухоли-альфа в сыворотке крови пациентов с опухолями костей. ГПки УкраТни Плюс 2016; (3): 75-78
13. Groblewska М, Mroczko В, Czygier М, Szmitkowski М. Cytokines as markers of osteolysis in the diagnostics of patients with bone metastases. Postepy Hig Med Dosw (Online) 2008; (62): 668-675
14. Коршунов Г.В., Павленко Н.Н., Пучиньян Д.М., Шахмартова С.Г. Содержание биомаркеров в сыворотке крови больных с новообразованиями костей. Саратовский научно-медицинский журнал 2016; 12(3): 358-361
15. Jung YS, Lee SO. Apomorphine suppresses TNF-a-induced MMP-9 expression and cell invasion through inhibition of ERK/AP-1 signaling pathway in MCF-7 cells. Biochem Biophys Res Commun 2017. May 2. pii: S0006-291X (17) 30840-9. doi:10.1016/j.bbrc.2017.04.151.

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