Saratov JOURNAL of Medical and Scientific Research

Application evaluation of a polydimethylsiloxane low-viscosity for cryopreservation and radiation sterilization of human cadaveric vascular allografts.

Year: 2016, volume 12 Issue: №4 Pages: 662-670
Heading: radiation medicine Article type: Original article
Authors: Lauk-Dubitsky S.E., Astrelina Т.А., Brumberg V.A., Fedyunin A.A., Kamyshnikov O.Yu., Vostrukhin S.V., Gorde'yev A.V., Paklina O.V., Kobzeva I.V., Nikitina V.A., Suchkova Yu.B., Usupzhanova D.Yu., Brunchukov V.A., Karaseva T.V., Bushmanov A.Yu., Samoilov A.S.
Organization: State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency, City Clinical Hospital n.a. S.P. Botkin

Aim: to investigate potential cryo- and radioprotective properties of a polydimethylsiloxane of low viscosity to provide safe and reliable cryopreservation and radiation sterilization of human cadaveric vascular allografts. Material and methods. A consecutive experimental set-up was carried out to establish polydimethylsiloxane (further — PDMS) protective endocellular properties. For this purpose a high-densitymesenchymal stem cells suspension was cryopreserved with 10% DMSO solution; PDMS exocellular protective properties were assessed by cyclic cryoconservation of ^femoral artery segments, while PDMS potential radioprotectiveproperties were assessed by gamma irradiation — induced sterilization of 3 cryopreserved femoral artery segments with gross length about 3cm, and for control grafts a slightly changed protocol with glycerin was used. In this study the dynamic viscosity of all cryopreserved vessels was analyzed with using specially designed experimental device, also electron scanning microscopy with lanthanide cells staining and routine hematoxylin — eosin cell staining were applied. Results. We have demonstrated a prominentexocellular protective effect of PDMS confirmed by a SEM and histological results, and also its mediated radioprotective effect, in particular because of its safer preliminary cryopreservation procedure. Conclusion. Use of PDMS for a cryopreservation and gamma sterilization showed its rationality, however requires further modification of protocols and additional researches.

1. Shangina OR. Morfologicheskie osnovi radiazionnoy ustoichivosti soedinitelnotkannikh transplantov: DSc abstract. Saransk, 2007; 25 p.
2. Shangina OR. Vliyanie radiazionnoy sterilizazii na strukturu biomaterialov: PhD abstract. Ufa, 1999; 30 p.
3. Li Z, Nambiar S, Zheng W, Yeow JTW. PDMS/single-walled carbon nanotube composite for proton radiation shielding in space applications. Materials Letters 2013; (108): 79-83
4. Lauk-DubitskiySE,AstrelinaTA,etal.Anovelcomprehensive approach for human vascular allografts cryopreservation and radiation sterilization for the tissue engineering industry. Saratov Journal of Medical Scientific Research 2015; 11 (4): 624-632
5. Mata A, Fleischman AJ, Roy S. Characterization of polydimethylsiloxane (PDMS) properties for biomedical micro/ nanosystems. Biomed Microdevices 2005; 7 (4): 281-293
6. Li H, LiX, LuoaC,etal. Icephobicityofpolydimethylsiloxane-b-poly (fluorinated acrylate). Thin Solid Films 2014; (573): 67-73
7. Palsule AS, Clarson SJ, et al. Gamma Irradiation of Silicones. Organometallic Polymers 2008; (18): 207
8. Novikov IA, Subbot AM, FedorovAA. Supravital lanthanoid staining for scanning electron microscopy of biological objects. Genes and cells 2015; (10) 2: 90-96
9. Rooney Р, Eagle М, Hogg Р, Lomas R, et al. Sterilization of skin allograft with gamma irradiation. Burns, 2008; 34 p.

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