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Cytogenetic examination in remote period after the stay in the Semipalatinsk nuclear test site area

Year: 2015, volume 11 Issue: №4 Pages: 620-624
Heading: Genetics Article type: Original article
Authors: Nugis V.Yu., Kozlova M.G.
Organization: State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency
Summary:

Aim: retrospective cytogenetic indication of doses probably received by individuals during the residence in the region of the Semipalatinsk nuclear test site, at work or service in the Armed Forces on it. Material and methods: the analysis of chromosomal aberrations in peripheral blood lymphocyte cultures of 16 people с using FISH-method. Re-sults. The frequencies of FISH-registered translocations were not significantly different from control values in 7 out of 16 cases; the dose evaluations ranged from 0.20 to 0.75 Sv for the remaining 9 persons. Conclusion. FISH-technique of chromosome aberration analysis allows to make retrospective dose assessment; however it is desirable simultaneously to produce classical analysis also for a more correct interpretation of the results; Detection of single multiaber-rant cells in peripheral blood lymphocyte cultures is characteristic of persons who were in the areas of alpha-emitting radionuclide pollution, what is allowed to talk about radiation damage, but does not allow to make dose assessment.

Keywords: chromosome aberrations, irradiation, peripheral blood lymphocyte culture, Semipalatinsk nuclear test site

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