Saratov JOURNAL of Medical and Scientific Research

Detection of Chlamydia trachomatis in blood samples as a diagnostic method for complicated and persistent forms of urogenital chlamydia infections

Summary:

Goal: the study of the effectiveness of the method for laboratory diagnostics of urogenital chlamydial infection in patients with chronic form of the disease. Material and methods. The presence of DNAof C. trachomatis was detected by PCR in either genital or extragenital (blood) sites in eighth patients (four men and four women). Results. It is established that in biological material taken from extragenital (blood) sites, C. trachomatis was detected in all patients examined (in 100% of cases), while in clinical samples obtained from genital sites, in seven patients only (87.5%). Conclusion. We found that specific chlamydial DNAcan be detected in extragenital (blood) site, despite the negative reaction in the clinical material from the genital tract of patients with genital chlamydial infection.

Bibliography:
1. WHO. Prevention and control of sexually transmitted infections: draft global strategy, 2006. URL: http://www.who.int/repro-ductive-health/docs/stis_st rategy.pdf
2. Dermatovenereology: clinical Guidelines / ed. by A.A. Kubanova. Moscow: ZAO FID Delovoi express, 2010; 413-425
3. Centers for Disease Control and Prevention. Sexually transmitted disease treatment guidelines, MMWR Morbs Mortal Wkly Rep.2010; 59 (RR-12): 1-110. Erratum in: MMWR Recomm Rep. 2011; 60 (1): 18
4. Fenton KA, Mecer CH, Johnson AM, et al. Reported sexually transmitted disease clinic attendance and sexually transmitted infections in Britain: prevalence, risk factors, and proportionate population burden. J Infect Dis2005; 191: 127-138
5. Pashko YP, Zigangirova NA, Kapotina LN, et al. Features of distribution in the organism Chlamydia trachomatis in the chronic course of urogenital chlamydia and pathogen detection in blood serum. Fundamental Research 2010; 7: 50-57
6. Zigangirova NA, Petyaev IM, Pashko YM, et al. Generalization of infection in patients with urogenital chlamydia infection. Clin Microbiol Antimicrob Chemioter 2007; 9 (4): 351-360
7. Pashko YP, Zigangirova N, Petyaev IM, et al. Modern aspects of diagnostics of chronic urogenital chlamydia sticks, called persistent forms of chlamydia. J Microbiol 2009; (4): 89-93
8. Pashko YP, Morgunova Е, Capatina LN, et al. Diagnosis of active persistent infections in patients with urogenital chlamyd-ia. In: Genodiagnostic infectious diseases 2007: Sat. works. VI all Russian scientific.-practical, conf.; M., 2007; vol.2, p. 181-183
9. Feodorova VA, Sultanakhmedov ES, Saltykov YV, et al. Testing the blood of patients with urogenital chlamydia infection increases the efficiency of laboratory diagnosis of Chlamydia trachomatis. Molecular diagnosis 2014; (1): 172-173
10. Safina ON. Urogenital chlamydia and chronic pros-tatitis. Sib Jur dermatol and venerol 2003; (4): 62-63
11. Peter N. Fitz-Hugh-Curtis syndrome: A diagnosis to consider in women with right upper quadrant pain. Cleveland Clinic Journal of Medicine 2004; 7 (3): 233-239
12. Mavrov Gl. Chlamydia trachomatis in the lumen of capillaries of the fallopian tubes: the possibility of hematogenous spread of infection. Phys The Academy of medical Sciences of Ukraine 1996; 4: 704-711
13. Of sexually transmitted infections: Clinic, diagnostics, treatment / ed. by VA. Molochkov; M.: Medicine, 2006; p. 384-413
14. Treatment protocol "Urogenital chlamydial infection" /ed. by VI. Kissina. M: Nyudiamed, 2011; p. 164-191
15. Lanjouw Е, Ossewaarde JM, Stary A, et al. 2010 European guidelines for the management of Chlamydia trachomatis infections. IntJSTD AIDS 2010; 21 (11): 729-37
16. Workowski KA, Berman S. Centers for Disease Control and Prevention (CDC). Sexually Transmitted Diseases Treatment Guidelines 2010. MMWR Recomm Rep 2010; 59 (RR-12): 1-10
18. Centers for Disease Control and Prevention (CDC). Recommendations for the Laboratory-Based Detection of Chlamydia trachomatis and Neisseria gonorrhoeae 2014. MMWR Recomm Rep, March 14, 2014; 63 (RR02): 1-19
19. Quint KD, van Doom LJ, Kleter B, et al. A Highly Sensitive, Multiplex broad-spectrum PCR-DNA-enzyme immunoassay and reverse hybridization assay for rapid detection and identification of Chlamydia trachomatis serovars. Molecular Diagnostics 2007;9:631-638
20. Domeika M, Savicheva A, Sokolovskiy E, et al. Guidelines for the laboratory diagnosis of Chlamydia trachomatis infections in East European countries. J Eur Acad Dermatol Venereol 2009 (Dec); 23 (12): 1353-1363
21. Geisler WM, Suchland RJ, Whittington WL, et al. The relationship of serovar to clinical manifestations of urogenital Chlamydia trachomatis infection. Sex Transm Dis 2003; 30 (2): 160-165
22. ChurakovAA, KulichenkoAN, Kazakova ES. To the question about laboratory diagnostics-the stick of urogenital chlamydia infection. Clinical and laboratory diagnostics 2005; (2): 43-47
23. Kubanova АА, Frigo NV, Rotanov SV, et al. Modern approaches and prospects of development of laboratory diagnostics of sexually transmitted infections. Journal of Dermatology and Venereology 2011; 5: 54-63
24. Gomes JP, Bruno WJ, NunesA, et al. Evolution of Chlamydia trachomatis diversity occurs by widespread interstrain recombination involving hotspots. Genome Res 2008; 17: 50-60
25. Stothard DR. Use of a reverse dot blot procedure to identify the presence of multiple serovars in Chlamydia trachomatis urogenital infection. J Clin Microbiol 2001; 39: 2655-2659
26. Spraaragen J, Verhaest I, Mooi JS, et al. Analysis of Chlamydia trachomatis. Serovar distribution changes in the Netherlands (1986-2002). Sex Transm Infect 2005; 80: 151-152. 27. Lister NA, Faitley CK. О trachomatis serovars causing urogenital infections in women in Melbourne, Australia. J Clin Microbiol 2005; 43: 2546-2547.

AttachmentSize
2015_03-01_381-385.pdf321.91 KB

No votes yet