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Dynamics of laser speckle imaging of blood flow and morphological changes in tissues with a full time local ischemia of pancreas

Year: 2014, volume 10 Issue: №4 Pages: 596-600
Heading: Macro- and Micromorphology Article type: Original article
Authors: Alexandrov D.A., Timoshina P.A., Tuchin V.V., Maslyakova G.N., Palatova T.V., Skorokhod A.A., Lukashevich K.A., Yarovoy A.S..
Organization: Saratov State Medical University, Saratov state university
Summary:

The purpose: to establish influence of a full ischemia of different duration and the subsequent reperfusionon pathology development in pancreas of rats by means of laser speckle-visualization and lifetime digital microscopy. Materials and Methods. The work has been performed on 42 white rats of line Wistar in weight of 200-250 Research of properties of a blood-groove was made by means of methods laser Doppler flowmetry, digital biomicroscopy and a method of laser speckle-contrast visualization. Results. After the termination of a 5-minute full ischemia the speed of bloodflow has been increased in 2-3 times, clinic pancreatic necrosis is marked does not develop. After the termination of 20-minute full ischemia the increase in speed of a bloodflow did not occur, there were morphological and clinical signs of pancreatic necrosis. Conclusion, the efficiency of monitoring of microhemodynamics of pancreas in rats by the method of speckle-capillary of full field has been shown. Multidirectional phase of perfusion changes in pancreas have been revealed after reversible infringement of blood supply of different duration.

Keywords: pancreas, reperfusion, speckle imaging

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All articles of the authors: Alexandrov D.A., Lukashevich K.A. , Maslyakova G.N., Palatova T.V. , Skorokhod A.A. , Timoshina P.A. , Tuchin V. V., Yarovoy A.S.

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