Saratov JOURNAL of Medical and Scientific Research

Comparative study of the amount of DNA single-strand breaks and alkali-labile sites in human peripheral blood lymphocytes exposed to 365 nm UV versus X-ray radiation

Year: 2013, volume 9 Issue: №4 Pages: 791-795
Heading: Genetics Article type: Original article
Authors: Smetanina N.M., Pustovalova М.V., Bushmanov A.Yu., Osipov A.N.
Organization: State Scientific Research Center n.a. A.I. Burnasyan — Federal Medical Biophysical Center of Federal Medical Biological Agency
Summary:

Aim: to compare the amount of DNA single-strand breaks (SSB) and alkali-labile sites (ALS) induced by DNA 365 nm ultraviolet (UV) versus X-rays radiation in human peripheral blood lymphocytes at in vitro conditions. Material and methods. Blood lymphocytes were isolated in a Ficoll-verografin density gradient. Irradiation of cells was performed using BLX-365 UV-unit (wavelength 365 ± 10nm)and RUSTM1 biological X-ray unit. Analysis of DNA SSB and ALS was carried out using comet assay at alkaline conditions. Results. For the first time it was demonstrated that the amount of SSB and ALS induced by 365 nm UV radiation at a dose of 10 kJ/m2 in human lymphocytes corresponds to the effect of X-rays at a dose of- 1 Gy. Conclusion. Exposure of human peripheral blood lymphocytes to 365 nm UV radiation at a dose of 1 kJ/m2 induces - 100 DNA SSB per cell.

Bibliography:
1. Chronic UVA irradiation of human HaCaT keratinocytes induces malignant transformation associated with acquired apoptotic resistance / Y.Y. He, J. Pi, J.L. Huang [et al.] // Oncogene. 2006. Vol. 25. P. 3680-3688; 2. Ridley A.J., Whiteside J.R., McMillan T. J., Allinson S. L. Cellular and sub-cellular responses to UVA in relation to carcinogenesis// Int. J. Radiat. Biol. 2009. Vol. 85 (3). P. 177-195; 3. UV irradiation increases ROS production via PKCdelta signaling in primary murine fibroblasts / O. Bossi, M. Gartsbein, M. Leitges [et al.] // Cell Biochem. 2008. Vol. 105 (1). P. 194-207; 4. Cooper K. L, Liu K.J., Hudson L. G. Enhanced ROS production and redox signaling with combined arsenite and UVA exposure: contribution of NADPH oxidase // Free Radical Biol. Med. 2009. Vol. 47 (4). P. 381-388; 5. Najafzadeh M., Baumgartner A., Gopalan R. [et al.] In vitro sensitivities to UVA of lymphocytes from patients with colon and melanoma cancers and precancerous states in the micronucleus and the Comet assays//Mutagenesis. 2012. Vol. 27 (3). P. 351-357; 6. DNA damage after acute exposure of mice skin to physiological doses of UVB and UVA light / A. R. Svobodova, A. Galandakova, J. Sianska [et al.] //Arch. Dermatol. Res. 2012. Vol. 304 (5). P. 407-412; 7. Smetanina N.M., Pustovalova M.V., Osipov A.N. Modificirovannyj metod DNK-galo dlja ocenki povrezhdenijDNK, inducirovannyhrazlichnymigenotoksicheskimi agentami // Radiacionnaja biologija: Radiojekologija. 2013. T. 53, № 4. S. 389-393; 8. Mascotti K., McCullough J., Burger S. R. HPC viability measurement: trypan blue versus acridine orange and propidium iodide // Transfusion. 2000. Vol. 40 (6). P. 693-696; 9. Ocenka molekuljarnyh i citogeneticheskih jeffektov hronicheskogo vozdejstvija nizkointensivnogo v-izluchenija u myshej / A. N. Osipov, A. L. Elakov, P. V Puchkov [i dr.] // Genetika. 2002. T. 38, № 10. S. 1345-1350; 10. Mjazin A. E., Osipov A. N., Elakov A.L., Sypin V.D., Shevchenko VA. Ocenka metodom DNK-komet odnonitevyh razryvov DNK limfocitov selezenki v otdalennye sroki posle ostrogo obluchenija myshej v subletal'nyh i sredneletal'nyh dozah // Radiacionnaja biologija: Radiojekologija. 2002. T. 42, vyp. 6. О 731-734; 11. Comparison in vivo study of genotoxic action of high versus very low dose-rate y-irradiation /A. N. Osipov, D.Y. Klokov, A. L. Elakov [et al.] // Nonlinearity in Biology, Toxicology and Medicine. 2004. Vol. 2, № 3. P. 223-232; 12. Roots R., Okada S. Protection of DNA molecules of cultured mammalian cells from radiation-induced single strand scissions by various alcohols and SH compounds // Int. J. of Radiat. Biol. 1972. Vol. 21. P. 329-342; 13. UVcausationofmelanomainXiphophorusisdominated by melanin photosensitized oxidant production / S. R. Wood, M. Berwick, R.D. Ley [et al.] // Proc. Natl. Acad.Sci. USA. 2006. Vol. 103. P. 4111-4115; 14. Kawanishi S., Hiraku Y. Sequence-specific DNA damage induced by UVA radiation in the presence of endogenous and exogenous photosensitizers// Curr. Probl. Dermatol. 2001. Vol. 29. P. 74-82; 15. Cadet J., Douki T. Oxidatively generated damage to DNA by UVA radiation in cells and human skin // J. Invest. Dermatol. 2011. Vol. 131 (5). P. 1005-1007; 16. Ridley A.J., Whiteside J.R., McMillan T. J., Allinson S. L. Cellular and sub-cellular responses to UVA in relation to carcinogenesis // Int. J. Radiat. Biol. 2009. Vol. 85 (3). P. 177-195; 17. Singlet oxygen induces oxidation of cellular DNA / J. L. Ravanat, P. Di Mascio, G. R. Martinez [et al.] // J. Biol. Chem. 2001. Vol. 276. P. 40601-40604; 18. Cadet J., Douki T., Ravanat J. L., Di Mascio P. Sensitized formation of oxidatively generated damage to cellular DNA by UVA radiation // Photochem. Photobiol. Sci. 2009. Vol. 8 (7). P. 903-911; 19. Ward J. F. DNA damage produced by ionizing radiation in mammalian cells: identities, mechanisms of formation, and reparability // Prog. Nucleic Acid Res.Mol. Biol. 1988. Vol. 35. P. 95-125.

AttachmentSize
2013_04-01_791-795.pdf323.3 KB

No votes yet